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Functional immunome of Aedes aegypti saliva

Grant number: 09/09892-6
Support type:Research Grants - Young Investigators Grants
Duration: May 01, 2010 - April 30, 2015
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Anderson de Sá Nunes
Grantee:Anderson de Sá Nunes
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):14/14379-4 - Mediators and mechanisms involved in the sensitization and desensitization processes to Aedes egypti mosquito bites, BP.DR
13/12467-0 - Effects of Aedes Aegypti salivary components on the biology of macrophages and potential therapeutical applications, BP.DR
13/00740-4 - Salivary peptides of Aedes aegypti, BP.DR
+ associated scholarships 12/11816-9 - Immunofunctional characterization of a lymphocyte inhibitor in Aedes aegypti saliva, BP.DR
11/15569-3 - Characterization of the biological activity of Aedes aegypti salivary serpin AET-7393, BP.MS
11/22669-4 - Immunomodulation of experimental autoimmune encephalomyelitis by salivary gland extract of Aedes aegypti, BP.DR
10/18216-1 - Role of host immunity on biological fitness of Aedes aegypti mosquito vector, BP.PD
10/00227-7 - Biochemical and immunological characterization of a salivary serpin from vector Aedes aegypti, BP.DR
09/12247-5 - Effect of Aedes aegypti saliva on the differentiation, maturation and function of dendritic cells, BP.MS - associated scholarships

Abstract

Two recent revolutions changed the history of Aedes aegypti mosquito research. The first occurred with the publication of the species' "sialomes". These catalogs, representing the proteins and messenger RNAs present in the salivary gland of this vector, increased the knowledge on the salivary constituents in more than twenty times. The second revolution came through the sequencing of its genome, creating a unique perspective for comparative studies and development of genomic tools for transcriptome analysis and reverse genetics. The current project aims to become a third revolution in the field by establishing the functional "immunome" of Ae. aegypti saliva. In this way, classic biochemical approaches (microfiltration, chromatographic separation, Edman's degradation, mass spectometry) and post genomic biochemical approaches (bioinformatic analysis, genetic cloning, protein expression, peptide synthesis) will be explored side by side in high-throughput immunopharmacological screenings, with the purpose of producing a map of the immunomodulatory activities from the proteins present in the saliva of this mosquito. This project will set up a new research line in the Department of Immunology from Institute of Biomedical Sciences - USP, where the work will be performed. Considering the impact of this vector and the diseases transmitted by it in the public health and in the worldwide economy, specially in developing countries, the knowledge generated by this project may lead to the discovery of new molecules with important biological activities and therapeutic potential, vaccine development against the pathogens transmitted by the mosquito and new approaches for vector control. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HENRIQUE, MARESSA O.; NETO, LEILA S.; ASSIS, JOSIANE B.; BARROS, MICHELE S.; CAPURRO, MARGARETH L.; LEPIQUE, ANA P.; FONSECA, DENISE M.; SA-NUNES, ANDERSON. Evaluation of inflammatory skin infiltrate following Aedes aegypti bites in sensitized and non-sensitized mice reveals saliva-dependent and immune-dependent phenotypes. IMMUNOLOGY, v. 158, n. 1, p. 47-59, SEP 2019. Web of Science Citations: 0.
BREIJO, MARTIN; ESTEVES, ELIANE; BIZZARRO, BRUNA; LARA, PRISCILA G.; ASSIS, JOSIANE B.; ROCHA, SERGIO; PASTRO, LUCIA; FERNANDEZ, CECILIA; MEIKLE, ANA; SA-NUNES, ANDERSON. Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages. PARASITES & VECTORS, v. 11, JUL 27 2018. Web of Science Citations: 2.
BARROS, MICHELE S.; GOMES, ELIANE; GUERONI, DANIELE I.; RAMOS, ANDERSON D.; MIROTTI, LUCIANA; FLORSHEIM, ESTHER; BIZZARRO, BRUNA; LINO, CIRO N. R.; MACIEL, CERES; LINO-DOS-SANTOS-FRANCO, ADRIANA; TAVARES-DE-LIMA, WOTHAN; CAPURRO, MARGARETH L.; RUSSO, MOMTCHILO; SA-NUNES, ANDERSON. Exposure to iBites Induces a Mixed-Type Allergic Response following Salivary Antigens Challenge in Mice. PLoS One, v. 11, n. 5 MAY 20 2016. Web of Science Citations: 1.
SALES-CAMPOS, HELIOSWILTON; DE SOUZA, PATRICIA REIS; BASSO, PAULO JOSE; RAMOS, ANDERSON DANIEL; NARDINI, VIVIANI; LAZO CHICA, JAVIER EMILIO; CAPURRO, MARGARETH LARA; SA-NUNES, ANDERSON; DE BARROS CARDOSO, CRISTINA RIBEIRO. Aedes aegypti salivary gland extract ameliorates experimental inflammatory bowel disease. International Immunopharmacology, v. 26, n. 1, p. 13-22, MAY 2015. Web of Science Citations: 8.
MACIEL, CERES; FUJITA, ANDRE; GUERONI, DANIELE I.; RAMOS, ANDERSON D.; CAPURRO, MARGARETH L.; SA-NUNES, ANDERSON. Evans Blue as a Simple Method to Discriminate Mosquitoes' Feeding Choice on Small Laboratory Animals. PLoS One, v. 9, n. 10 OCT 21 2014. Web of Science Citations: 3.
BIZZARRO, BRUNA; BARROS, MICHELE S.; MACIEL, CERES; GUERONI, DANIELE I.; LINO, CIRO N.; CAMPOPIANO, JULIA; KOTSYFAKIS, MICHALIS; AMARANTE-MENDES, GUSTAVO P.; CALVO, ERIC; CAPURRO, MARGARETH L.; SA-NUNES, ANDERSON. Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology. PARASITES & VECTORS, v. 6, NOV 15 2013. Web of Science Citations: 21.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.