Advanced search
Start date
Betweenand

Mycobacterium tuberculosis: genotyping, resistance profile and analysis of mutations from clinical isolates

Grant number: 09/53292-3
Support type:Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)
Duration: July 01, 2010 - November 30, 2012
Field of knowledge:Biological Sciences - Microbiology
Cooperation agreement: CNPq - PPSUS
Principal Investigator:Clarice Queico Fujimura Leite
Grantee:Clarice Queico Fujimura Leite
Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

This project aims to understand more about TB that affects diverse populations surrounding of São Paulo (São Paulo, Araraquara) and Mato Grosso do Sul (indigenous and non-indigenous) states, evaluated patients with pulmonary tuberculosis treated in their respective Arnbulatories and Health Services. It will be performed: 1) sputum baciloscopy; 2) Mycobacteria isolation through CULTIVO (BACTEC and LI); 3) species identification through biochemical proof and molecular techniques (PCR and PRA); 4) determining resistance profile/sensibility of mycobacteria strains identified as M tuberculosis against isoniazid and rifampicin drugs; 5) determining the drugs MIC against the clinical isolates of M. tuberculosis; 6) to perform SSCP technique of resistant strains: 7) to perform sequencing of resistance genes to evaluate mutations; 8) to perform molecular typing of M. tuberculosis strains through of MIRU and spoligotyping techniques and 9) to correlate the molecular epidemiology results with classical epidemiology data with the assembly of a database. (AU)