Development of new method for detection of anti-aquaporin-4 for the study of neuromyelitis optica

• Grant number: 12/50839-4
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2013
• End date: October 31, 2015
• Field of knowledge: Health Sciences - Medicine
• Agreement: CNPq - First Projects Program
• Principal Investigator: Doralina Guimarães Brum Souza
• Grantee: Doralina Guimarães Brum Souza
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The neuromyelitis optica (NMO) is inflammatory diseases, autoimmune disorder with predilection for optic nerve and spinal cord mediated by antibodies to aquaporin 4 (AQP4), a protein of the water channels with high expression in the CNS. The anti- aquaporin 4 (NMO-IgG) was discovered in 2004 and in addition to being diagnostic for the spectrum of NMO is related to pathogenesis of disease. We have about five diagnostic tests using different techniques and with the specificity 91-100%, but the sensitivity also around 50% to 70%. Thus, 50% to 30% of patients with optic neuromyelitis not confirm the autoimmune response by means of serological tests. The emphasis of the project is to develop a specific nanonbiossensor to interact with antiaquaporin 4 antibody. The detection of NMO-IgG in the serum of patients with optic neuritis and myelitis entails early treatment to prevent or slow new episode since the predictive value of the antibody is high. Treatment of NMO is based on monoclonal antibodies and immunosuppressants, which involves treatment of high cost and often judicialization. Thus, the development of new methods more sensitive and specific detection of anti-aquaporin 4 can result in better defined orientation early diagnosis and treatment. The purpose of this project is to develop a new diagnostic method to detect this antibody, to compare to standardize test detection of antibody research antiaquaporina 4 (AQP4). This methodology can be an alternative to increase sensitivity when compared to methods already in use. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, GUEDMILLER S.; IERICH, JESSICA C. M.; MORAES, ARIANA S.; SILVA, GISELA B. R. F.; LIU, YANYUN; NETO, LOURIVAL R. DE S.; FARIA, ROBERTO R.; FRANCA, EDUARDO F.; FREITAS, LUIZ C. G.; BRIGGS, JAMES M.; et al. Immobilization and unbinding investigation of the antigen -antibody complex using theoretical and experimental techniques. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 86, p. 219-227, JAN 2019. (12/50839-4, 15/05283-6, 13/09746-5, 14/21530-0, 14/12082-4, 14/26369-3, 15/06847-0)

GARCIA, PAMELA SOTO; BRUM, DORALINA GUIMARAES; OLIVEIRA JR, OSVALDO N.; HIGA, AKEMI MARTINS; MAGALHAES IERICH, JESSICA CRISTIANE; MORAES, ARIANA DE SOUZA; SHIMIZU, FLAVIO MAKOTO; OKUDA-SHINAGAWA, NANCY M.; PERONI, LUIS ANTONIO; DA GAMA, PAULO DINIZ; et al. Nanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis. ULTRAMICROSCOPY, v. 211, APR 2020. (14/50869-6, 12/50839-4, 13/14262-7, 14/12082-4, 14/21530-0, 15/06847-0, 15/14360-4)

MAGALHAES IERICH, JESSICA CRISTIANE; BRUM, DORALINA GUIMARAES; MORAES, ARIANA DE SOUZA; HIGA, AKEMI MARTINS; GARCIA, PAMELA SOTO; MIYAZAKI, CELINA MASSUMI; FERREIRA, MARYSTELA; PERONI, LUIS ANTONIO; DE OLIVEIRA, GUEDMILLER SOUZA; FRANCA, EDUARDO DE FARIA; et al. Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes. SCIENTIFIC REPORTS, v. 9, FEB 14 2019. (13/09746-5, 14/12082-4, 14/15093-7, 14/21530-0, 12/50839-4, 15/05283-6, 14/26369-3, 14/50869-6, 15/06847-0, 16/19387-0)

MORAES, ARIANA DE SOUZA; BRUM, DORALINA GUIMARAES; MAGALHAES IERICH, JESSICA CRISTIANE; HIGA, AKEMI MARTINS; JABUR ASSIS, AMANDA STEFANIE; MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; PERONI, LUIS ANTONIO; MACHINI, M. TERESA; BARREIRA, AMILTON ANTUNES; et al. A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders. SCIENTIFIC REPORTS, v. 9, NOV 6 2019. (16/19387-0, 14/50869-6, 13/14262-7, 14/15093-7, 12/50839-4, 14/12082-4, 15/05283-6, 15/06847-0, 15/14360-4)