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Enhancing the etiologic diagnosis of leishmaniasis in the state of São Paulo: basis for the deployment of a network of laboratories to identify the routes of dissemination and monitoring of phenotypic and genotypic diversity of protozoa of the genus Leishmania

Abstract

This research project arose from integration efforts involving the Instituto Adolfo Lutz, São Paulo State University, Campus of Presidente Prudente, Services Zoonosis Control of Jundiaí, Bauru and Caraguatatuba, further cooperation CLlO / FIOCRUZ in response the need to review the policy on epidemiological surveillance and control of leishmaniasis Improve the etiologic diagnosis of leishmaniasis in the state of São Paulo, Brazil; study the phenotypic and genotypic diversity of Leishmania spp in the state of São Paulo, Brazil. To this end we will establish and empower a network of laboratories to perform activities of isolation and identification of Leishmania spp circulating in different hosts, vertebrate and invertebrate, in the state of São Paulo, standardize and establish the methodologies necessary for growth and isolation of primary Leishmania spp; perform the reaction in situ hybridization to search for Leishmania, as a complementary diagnostic morphological examination by hematoxylin and eosin (HE) in canine samples; deploy the reference methodologies for the identification of phenotypic diversity (isozymes) of Leishmania spp; perform the methodologies for identification of genotypic diversity (PCR, real time PCR, microsatellite, sequencing) of Leishmania spp; construct maps of the distribution and identify routes of geographical dispersion of Leishmania spp and leishmaniasis in the state of São Paulo; organize and deploy the Reference Collection of Leishmania in the state of São Paulo, the Center for integrated Biological Resources in Health / Center for Biological Collections of the Instituto Adolfo Lutz, research and service. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUERR, J. M.; FERNANDES, N. C. C. A.; RESSIO, R. A.; MAGNO, J. A.; KIMURA, L. M.; BARBOSA, J. E. DE R.; BERTOLLO, D. M. B.; TANIGUCHI, H. H.; HIRAMOTO, R. M.; MOTOIE, G.; TOLEZANO, J. E.; COGLIATI, B. Evaluation of Cytopathological Techniques for the Diagnosis of Canine Visceral Leishmaniosis with Lymph Node Samples. Journal of Comparative Pathology, v. 172, p. 62-71, OCT 2019. Web of Science Citations: 0.
DONALISIO, MARIA RITA; PAIZ, LAIS MORAES; DA SILVA, VANESSA GUSMON; RICHINI-PEREIRA, VIRGINIA BODELAO; BRUNO VON ZUBEN, ANDREA PAULA; CASTAGNA, CLAUDIO LUIZ; MOTOIE, GABRIELA; HIRAMOTO, ROBERTO MITSUYOSHI; TOLEZANO, JOSE EDUARDO. Visceral leishmaniasis in an environmentally protected area in southeastern Brazil: Epidemiological and laboratory cross-sectional investigation of phlebotomine fauna, wild hosts and canine cases. PLoS Neglected Tropical Diseases, v. 11, n. 7 JUL 2017. Web of Science Citations: 4.
PAIZ, LAIS MORAES; DONALISIO, MARIA RITA; RICHINI-PEREIRA, VIRGINIA BODELAO; MOTOIE, GABRIELA; CASTAGNA, CLAUDIO LUIZ; TOLEZANO, JOSE EDUARDO. Infection by Leishmania spp. in Free-Ranging Opossums (Didelphis albiventris) in an Environmentally Protected Area Inhabited by Humans in Southeastern Brazil. VECTOR-BORNE AND ZOONOTIC DISEASES, v. 16, n. 11, p. 728-730, NOV 2016. Web of Science Citations: 2.

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