Study of scFv-therapy that mimicking the gp43 antigen from Paracoccidioides brasiliensis combined with nanoparticle in experimental paracoccidioidomycosis

• Grant number: 13/08548-5
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2013 - June 30, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Karen Spadari Ferreira
• Grantee: Karen Spadari Ferreira
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

The paracoccidioidomycosis is a deep mycosis, caused by Paracoccidioides brasiliensis (Pb) that attack the lung primarily. This fungus produce a several molecules and the gp43 is the main antigenic component of the Pb. Because the importance of the gp43, monoclonal antibodies were done. Based in the idiotypic network hypothesis, Jerne (1974) suggested that the antibody produced in response to some antigen, induce the others antibody production against the variable chain. Antibodies against idiotopes that recognize the paratope, are known Ab2- beta. This fragment has been used in several therapeutic models (PAN, et al., 1995, BONA, 1996 e HERLYN et al., 1996). In spite of the antibody to be a complex molecule, we know that the Fab region is the responsible to mimmick the antigen. Our group (FERREIRA, et al., 2011) engineered a new molecule, that mimmicking the gp43 antigen of Paracoccidioides brasiliensis, the single chain fragment variable (scFv). The transfection of the scFv in dendritic cells was competent in the experimental PCM treatment. The scFv trasfected in dendritic cells was capable to induce T cells response and reduced the unit forming colonies in the lung. On the order hand, the nanoparticle can stimulate for a long time the scFv in the animal. So, the aim of this work is to insert the scFv in the nanoparticle to high the efficiency of the therapy in experimental PCM. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JANNUZZI, GRASIELLE PEREIRA; TAVARES, ALDO HENRIQUE F. P.; KAIHAMI, GILBERTO HIDEO; FOGACA DE ALMEIDA, JOSE ROBERTO; DE ALMEIDA, SANDRO ROGERIO; FERREIRA, KAREN SPADARI. scFv from Antibody That Mimics gp43 Modulates the Cellular and Humoral Immune Responses during Experimental Paracoccidioidomycosis. PLoS One, v. 10, n. 6, JUN 19 2015. (13/08548-5)

JANNUZZI, GRASIELLE PEREIRA; SOUZA, NICOLE DE ARAUJO; FRANCOSO, KATIA SANCHES; PEREIRA, RONEY HENRIQUE; SANTOS, RAQUEL POSSEMOZER; KAIHAMI, GILBERTO HIDEO; FOGACA DE ALMEIDA, JOSE ROBERTO; BATISTA, WAGNER LUIZ; AMARAL, ANDRE CORREA; MARANHAO, ANDREA QUEIROZ; et al. Therapeutic treatment with scFv-PLGA nanoparticles decreases pulmonary fungal load in a murine model of paracoccidioidomycosis. Microbes and Infection, v. 20, n. 1, p. 48-56, JAN 2018. (13/08548-5)