Transcriptome analysis of sugar cane leaves under extended water shortage, using RNA-Seq

• Grant number: 13/11617-9
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2013
• End date: September 30, 2015
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Maria Inês Tiraboschi Ferro
• Grantee: Maria Inês Tiraboschi Ferro
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Jairo Osvaldo Cazetta ; Jesus Aparecido Ferro ; Poliana Fernanda Giachetto ; Samira Domingues Carlin Cavallari

Abstract

In Brazil the culture of sugarcane has expanded to areas with prolonged periods of water shortage, which has limited the production. The best way to avoid this problem is the use of stress-tolerant cultivars. This study aims the analysis of the molecular behavior of plants subjected to different periods of water limitation, and to determine the variables which could be used, independently or in combination, as an effective tool to differentiate and identify cultivars tolerant to prolonged water stress. It also expects to generate new knowledge to promote the development and competitiveness of this culture, and contribute to the formation of specialized human resources in this area. To achieve this objective, plants of two contrasting cultivars (tolerant and sensitive) will be grown in 50-liter pots in a greenhouse and subjected to three well controlled soil water potentials (assay composed by 6 treatments and 3 replications). The molecular parameters will be monitored at 30, 60 and 90 days after treatment application. After that, molecular analysis will be carried out using the technique of RNA-Seq for identifying differentially expressed genes in the cultivars under different treatments, and some of them validated by real time PCR. The results of this proposal will be important for better understanding the responses and tolerance mechanisms of these plants to prolonged water stress and help establishing secure methods to identify and recommend cultivars which are really tolerant to water limitation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BELESINI, A. A.; CARVALHO, F. M. S.; TELLES, B. R.; DE CASTRO, G. M.; GIACHETTO, P. F.; VANTINI, J. S.; CARLIN, S. D.; CAZETTA, J. O.; PINHEIRO, D. G.; FERRO, M. I. T.. De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress. Genetics and Molecular Research, v. 16, n. 2, MAY 26 2017. (13/22122-0, 13/11617-9)