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Evaluation of apoptosis induction on Schistosoma mansoni adult worm after exposure with curcumin

Grant number: 13/11164-4
Support type:Regular Research Grants
Duration: October 01, 2013 - September 30, 2015
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Lizandra Guidi Magalhães
Grantee:Lizandra Guidi Magalhães
Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
Assoc. researchers:Vanderlei Rodrigues

Abstract

Schistosomiasis remains a serious public health problem in many developing countries. Reports show an increasing emergence of Schistosoma mansoni strains resistant to Praziquantel, the current drug of choice to the Schistosomiasis treatment.Recently, it was identified and characterized the apoptosis pathway mediated by Bcl-2 on Schistosoma mansoni and Schistosoma japonicum, suggesting this pathway as a new target for drugs schistosomicidal. Previous studies have shown that curcumin, which is a compound extracted from the rhizome of Curcuma longa plant has activity both in vitro and in vivo. Furthermore, studies with other organisms have shown curcumin generates reactive oxygen species (ROS) and induces apoptosis. Thus, this study aims to evaluate the induction of apoptosis in adult worms of S. mansoni. For this purpose, it will evaluate the effect of the compound on the viability of the parasites to determine the concentrations and time that will be used in the other experiments. Through microscopy, molecular and biochemical methods will be evaluated the morphology of the parasites, DNA fragmentation, the anti-apoptotic and pro-apoptotic gene expression and enzymatic activity for caspase 3 and 7 on S. mansoni adult worms after incubation with curcumin. In order to evaluate oxidative stress, some parameters will be determined as the activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx) Glutathione Reductase (GR) and glutathione S transferase (GST), and will be determined the anion superoxide production and the levels of protein carbonyl. Thus, this research project will promote inter and multidisciplinary development which will be important to understand of the biology of the parasite and mechanisms by curcumin exerts it's in vitro schistosomicidal effect. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MATOS, JAQUELINE L.; DA SILVA, KAREN R.; DE LIMA PAULA, LUCAS A.; CUNHA, WILSON R.; RAMOS, SALVADOR B.; RODRIGUES, VANDERLEI; CABRAL, FERNANDA J.; MAGALHAES, LIZANDRA G. Molluscicidal and cercaricidal activities of curcumin on Biomphalaria glabrata and Schistosoma mansoni cercariae. Pest Management Science, v. 76, n. 4 NOV 2019. Web of Science Citations: 0.
DIAS, HERBERT J.; PATROCINIO, ANDRESSA B.; PAGOTTI, MARIANA C.; FUKUI, MURILO J.; RODRIGUES, VANDERLEI; MAGALHAES, LIZANDRA G.; CROTTI, ANTONIO E. M. Schistosomicidal Activity of Dihydrobenzofuran Neolignans. CHEMISTRY & BIODIVERSITY, v. 15, n. 7 JUL 2018. Web of Science Citations: 4.
SOUZA, RITIERES LOVO; GONCALVES, UBIRAJARA OLIVEIRA; BADOCO, FERNANDA RAFACHO; GALVAO, LUCAS DE SOUZA; DOS SANTOS, RAQUEL ALVES; DIAS DE CARVALHO, PAULO HENRIQUE; ALEIXO DE CARVALHO, LARA SOARES; DA SILVA FILHO, ADEMAR ALVES; SOLA VENEZIANI, RODRIGO CASSIO; RODRIGUES, VANDERLEI; AMBROSIO, SERGIO RICARDO; MAGALHAES, LIZANDRA GUIDI. Licochalcone A induces morphological and biochemical alterations in Schistosoma mansoni adult worms. BIOMEDICINE & PHARMACOTHERAPY, v. 96, p. 64-71, DEC 2017. Web of Science Citations: 2.
KAPADIA, GOVIND J.; SOARES, INGRID A. O.; RAO, G. SUBBA; BADOCO, FERNANDA R.; FURTADO, RICARDO A.; CORREA, MARIANA B.; TAVARES, DENISE C.; CUNHA, WILSON R.; MAGALHAES, LIZANDRA G. Antiparasitic activity of menadione (vitamin K-3) against Schistosoma mansoni in BABL/c mice. Acta Tropica, v. 167, p. 163-173, MAR 2017. Web of Science Citations: 5.
AGUIAR, DANIELA DE PAULA; MOREIRA MOSCARDINI, MAYARA BRUNETTO; MORAIS, ENYARA REZENDE; DE PAULA, RENATO GRACIANO; FERREIRA, PEDRO MANUEL; AFONSO, ANA; BELO, SILVANA; OUCHIDA, AMANDA TOMIE; CURTI, CARLOS; CUNHA, WILSON ROBERTO; RODRIGUES, VANDERLEI; MAGALHAES, LIZANDRA GUIDI. Curcumin Generates Oxidative Stress and Induces Apoptosis in Adult Schistosoma mansoni Worms. PLoS One, v. 11, n. 11 NOV 22 2016. Web of Science Citations: 7.
DE PAULA, RENATO GRACIANO; DE MAGALHAES ORNELAS, ALICE MARIA; MORAIS, ENYARA REZENDE; GOMES, MATHEUS DE SOUZA; AGUIAR, DANIELA DE PAULA; MAGALHAES, LIZANDRA GUIDI; RODRIGUES, VANDERLEI. Proteasome stress responses in Schistosoma mansoni. Parasitology Research, v. 114, n. 5, p. 1747-1760, MAY 2015. Web of Science Citations: 4.

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