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Prospection of anti-parasitic and anti-tumor metabolites from plant species of the Atlantic Forest

Grant number: 13/16320-4
Support type:Regular Research Grants
Duration: November 01, 2013 - October 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal Investigator:Patricia Sartorelli
Grantee:Patricia Sartorelli
Home Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
Assoc. researchers:Alisson Leonardo Matsuo ; André Gustavo Tempone Cardoso ; João Henrique Ghilardi Lago


In the previous project "Principles with potential anti-Leishmania species in the Atlantic Forrest: evaluation, isolation and molecular characterization" was evaluated the therapeutic potential of medicinal plant extracts derived from the Atlantic Forest in the treatment of cutaneous and visceral leishmaniasis. Thus, we selected several plant species with potential for the discovery of anti-Leishmania substances by studying the in vitro efficacy of extracts, fractions and pure compounds obtained from plant species collected in the Atlantic Forest against promastigote and amastigote forms of Leishmania chagasi and L. amazonensis.In this project we intend to continue the isolation of substances responsible for the activity detected in extracts using bioassay-guided fractionation by testing anti-Leishmania activity, in addition to expanding the evaluation to another anti-parasitic activity against Trypanosoma cruzi in particular, and evaluate also cytotoxic activity against the various tumor cell lines. Additionally we intend to conduct specific tests to define the mode of action of substances with proven anti-parasitic activity in relation to the parasite, particularly the evaluation of membrane pore and ultrastructural studies, and for that show anti-tumor activity will be conducted specific tests to define the mechanism of action and effect on metabolic processes related to cancer development.For the isolation and molecular characterization of the active principles in the extract will perform the chromatographic fractionation, using various separation techniques followed by spectroscopic analysis to determine structural bioactive substances. Thus, this project aims to add new compounds with proven anti-parasitic activity restricted to the therapeutic arsenal, providing tools for the development of new drugs for these neglected diseases, and presents new prototypes of drugs that can be used in cancer therapy (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA-SILVA, GUILHERME ALVARO; LAGES, CARLA CAROLINA LOPES; SARTORELLI, PATRICIA; HASEGAWA, FLAVIA RIE; SOARES, MARISI GOMES; IONTA, MARISA. Casearin D inhibits ERK phosphorylation and induces downregulation of cyclin D1 in HepG2 cells. TOXICOLOGY IN VITRO, v. 38, p. 27-32, FEB 2017. Web of Science Citations: 5.
AMARAL-BAROLI, ADRIANA; LAGO, JOAO HENRIQUE G.; DE ALMEIDA, CRISTINA V.; DE ALMEIDA, MARCILIO; SCOTTI, MARCUS TULLIUS; LEONE, GABRIELA F.; SOARES, MARISI G.; CAVALARI, ALINE A.; SARTORELLI, PATRICIA. Variability in essential oil composition produced by micropropagated (in vitro), acclimated (ex vitro) and in-field plants of Ocimum basilicum (Lamiaceae). INDUSTRIAL CROPS AND PRODUCTS, v. 86, p. 180-185, AUG 2016. Web of Science Citations: 3.
BOU, DIEGO D.; DOS SANTOS, AUGUSTO L.; FIGUEIREDO, CARLOS R.; FARIAS, CAMYLA F.; MATSUO, ALISSON L.; KITAMURA, RODRIGO O. S.; GIMENES, LEILA; LAGO, JOAO H. G.; SARTORELLI, PATRICIA. Dinor Casearin X, a New Cytotoxic Clerodane Diterpene from Casearia sylvestris. Journal of the Brazilian Chemical Society, v. 26, n. 8, p. 1725-1729, AUG 2015. Web of Science Citations: 3.
DIEGO D. BOU; AUGUSTO L. DOS SANTOS; CARLOS R. FIGUEIREDO; CAMYLA F. FARIAS; ALISSON L. MATSUO; RODRIGO O. S. KITAMURA; LEILA GIMENES; JOÃO H. G. LAGO; PATRICIA SARTORELLI. Dinor Casearin X, a New Cytotoxic Clerodane Diterpene from Casearia sylvestris. Journal of the Brazilian Chemical Society, v. 26, n. 8, p. -, Ago. 2015.
MORAIS, THIAGO R.; DA COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; BORBOREMA, SAMANTA ETEL T.; SCOTTI, MARCUS T.; DE SOUSA, RAQUEL MARIA F.; ARAUJO, ANA CAROLINA C.; DE OLIVEIRA, ALBERTO; DE MORAIS, SERGIO ANTONIO L.; SARTORELLI, PATRICIA; LAGO, JOAO HENRIQUE G. Antiparasitic Activity of Natural and Semi-Synthetic Tirucallane Triterpenoids from Schinus terebinthifolius (Anacardiaceae): Structure/Activity Relationships. Molecules, v. 19, n. 5, p. 5761-5776, MAY 2014. Web of Science Citations: 8.
BOU, DIEGO DINIS; TEMPORRE, ANDRE G.; PINTO, ERIKA G.; LAGOA, JOAO HENRIQUE G.; SARTORELLIA, PATRICIA. Antiparasitic activity and effect of casearins isolated from Casearia sylvestris on Leishmania and Trypanosoma cruzi plasma membrane. Phytomedicine, v. 21, n. 5, p. 676-681, APR 15 2014. Web of Science Citations: 19.

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