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Characterization of the microbiota-mediated anti-dengue mechanisms in wild Aedes aegypti populations

Grant number: 13/11343-6
Support type:Research Grants - Young Investigators Grants
Duration: November 01, 2013 - January 31, 2019
Field of knowledge:Biological Sciences - Parasitology
Cooperation agreement: Keele University
Principal Investigator:Jayme Augusto de Souza-Neto
Grantee:Jayme Augusto de Souza-Neto
Home Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Assoc. researchers:Diego Peres Alonso ; Julien Pelletier ; Paulo Eduardo Martins Ribolla
Associated grant(s):17/14348-0 - The FAPESP-Keele network phase 3: understanding the relationship between drought and dengue epidemics: the role of the FOXO pathway on hydric stress, immune responses and vector competence of Aedes aegypti mosquitoes, AV.EXT
16/13790-8 - The FAPESP-Keele network phase 2: exploiting a CRISPR-Cas9 platform for the generation of immune- and olfaction-mutant mosquitoes, AV.EXT
15/12401-5 - The FAPESP-Keele network phase 1: functional comparative analysis of the olfactory and immune systems of the dengue vectors Aedes aegypti and Aedes albopictus and establishment of a CRISPR-Cas9 mosquito mutagenesis platform, AV.EXT
14/50454-0 - A lab system to study Plasmodium vivax transmission and interactions with the vector, AP.R
Associated scholarship(s):18/14760-0 - The role of the PI3K/Akt/FoxO pathway in the modulation of the midgut microbial diversity of Aedes aegypti, BP.IC
16/16952-9 - Aedes aegypti global gene expression modulated by anti-DENV bacteria and identification of dengue virus restriction factors (DVRFs), BP.DD
15/26199-3 - The FAPESP-Keele network phase 2: generation of mutant Aedes aegypti lines using CRISPR-Cas9, BE.PQ
+ associated scholarships 16/14798-2 - Determination of Aedes aegypti natural populations susceptibility to dengue virus , BP.IC
14/25287-3 - The FAPESP-UNESP-Keele University cooperative network: fostering Brazil and UK interactions through joint reciprocal academic activities, BE.PQ
13/24518-9 - Characterization of the microbiota-mediated anti-dengue mechanisms in wild Aedes aegypti populations, BP.JP - associated scholarships

Abstract

Dengue is the arboviral disease of highest public health relevance due to its recent expansion in urban areas worldwide. Since vaccines and drug therapies are still not available and mosquito control programs have been insufficient to impair dengue outbreaks, the development of new tools for dengue control is urgently needed. However, this is limited by the poor understanding of the molecular mechanisms that govern virus-vector interactions. Mosquitoes have their midguts colonized by a symbiotic microbiota, which directly influences several physiological processes, including immune system maturation and protection against pathogens. Recent studies suggest that the gut microbiota is an important factor affecting mosquito's vector competence. Removal of these microbes through an antibiotic treatment leads to higher loads of dengue virus (DENV) in the Ae. aegypti midgut. Nonetheless, this mechanism is still widely unknown. In this scenario, this work aims to elucidate the factors related to the microbiota-mediated anti-DENV response in wild Ae. aegypti populations. The execution of this proposal will significantly improve our understanding of the complexes interactions between DENV and its vector. Furthermore, through the identification of anti-DENV bacteria, it has the potential to lead to the isolation of new antiviral compounds and the generation of mosquito lines refractory to the virus. These events would contribute directly to decrease dengue transmission in endemic areas. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Gut bacteria from Aedes aegypti could be weapon against dengue and other diseases 
Study identifies biomarkers of infection by Zika virus in <i>Aedes</i> mosquitoes 
Brazilian researchers participate in a scientific forum in Kyoto 
Gut bacteria of <i>Aedes aegypti</i> could become a weapon against dengue 
Articles published in Pesquisa FAPESP Magazine about the research grant:
Complementary Expertise 

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PASCOLI, MONICA; DE ALBUQUERQUE, FELICIA PEREIRA; CALZAVARA, ANDERSON KIKUCHI; TINOCO-NUNES, BRUNO; CRUZ OLIVEIRA, WANDERSON HENRIQUE; GONCALVES, KELLY CRISTINA; POLANCZYK, RICARDO ANTONIO; DELLA VECHIA, JAQUELINE FRANCIOSI; SOARES DE MATOSS, SIDNEIA TEREZINHA; DE ANDRADE, DANIEL JUNIOR; OLIVEIRA, HALLEY CAIXETA; SOUZA-NETO, JAYME A.; DE LIMA, RENATA; FRACETO, LEONARDO FERNANDES. The potential of nanobiopesticide based on zein nanoparticles and neem oil for enhanced control of agricultural pests. JOURNAL OF PEST SCIENCE, v. 93, n. 2 JAN 2020. Web of Science Citations: 0.
COSTA, JONAS HENRIQUE; BAZIOLI, JAQUELINE MORAES; ARAUJO, EDER DE VILHENA; VENDRAMINI, PEDRO HENRIQUE; DE FREITAS PORTO, MARIANA CRISTINA C.; EBERLIN, MARCOS NOGUEIRA; SOUZA-NETO, JAYME A.; FILL, TAICIA PACHECO. Monitoring indole alkaloid production by Penicillium digitatum during infection process in citrus by Mass Spectrometry Imaging and molecular networking. FUNGAL BIOLOGY, v. 123, n. 8, p. 594-600, AUG 2019. Web of Science Citations: 2.
CARLOS, BIANCA C.; RONA, LUISA D. P.; CHRISTOPHIDES, GEORGE K.; SOUZA-NETO, JAYME A. A comprehensive analysis of malaria transmission in Brazil. PATHOGENS AND GLOBAL HEALTH, v. 113, n. 1, p. 1-13, JAN 2 2019. Web of Science Citations: 2.
SOUZA-NETO, JAYME A.; POWELL, JEFFREY R.; BONIZZONI, MARIANGELA. Aedes aegypti vector competence studies: A review. INFECTION GENETICS AND EVOLUTION, v. 67, p. 191-209, JAN 2019. Web of Science Citations: 16.
ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; GUERREIRO, TATIANE MELINA; ESTEVES, CIBELE ZANARDI; BECK, RAISSA MARQUES; PADILLA, MARINA AIELLO; MILANEZ, GUILHERME PAIER; ARNS, CLARICE WEIS; PROENCA-MODENA, JOSE LUIZ; SOUZA-NETO, JAYME AUGUSTO; CATHARINO, RODRIGO RAMOS. A Lipidomics Approach in the Characterization of Zika-Infected Mosquito Cells: Potential Targets for Breaking the Transmission Cycle. PLoS One, v. 11, n. 10 OCT 10 2016. Web of Science Citations: 13.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.