Participation of NADPH oxidase 2 induced by estrogen in the formation of DNA damage and autophagy regulation in breast cells

• Grant number: 13/21075-9
• Support Opportunities: Research Grants - Visiting Researcher Grant - Brazil
• Start date: April 01, 2014
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Carlos Frederico Martins Menck
• Grantee: Carlos Frederico Martins Menck
• Visiting researcher: Rodrigo Soares Fortunato
• Visiting researcher institution: Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Biofísica Carlos Chagas Filho (IBCCF), Brazil
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 09/52417-7 - Cell responses to genome damage, AP.TEM

Abstract

The role of reactive oxygen species (ROS) in carcinogenesis is well described, but the source of these molecules in breast cancer remains controversial. The cells normally produce ROS as a by-product of the reactions catalyzed by xanthine oxidase and ciclooxigenase, among others, and the electron escape from mitochondria respiratory chain. On the other hand, there are enzymes, called NADPH Oxidases (NOX), which are specialized in ROS production. Studies about the relationship between NOX activity and cancer development are recent and until now there are no studies showing the involvement of NOX as a risk factor for the development of breast cancer. So, we aim to evaluate whether the oxidative stress induced by estrogen in mammary cells involves the regulation of NOX expression and activity. We will also try to elucidate the functional consequences of oxidative stress induced by NOX, such as oxidative DNA damage and cell survival or death related mechanisms. Thus, we aim to give new insight into the underlying molecular mechanisms of breast cancer development and progression, which can be useful for the development of new tools to treat and prevent breast cancer. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUINET, ANNABEL; LERNER, LETICIA K.; MARTINS, DAVI J.; MENCK, CARLOS F. M.. Filling gaps in translesion DNA synthesis in human cells. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, n. B, SI, p. 127-142, DEC 2018. (14/15982-6, 13/21075-9)

FORTUNATO, RODRIGO S.; GOMES, LUCIANA R.; MUNFORD, VERIDIANA; PESSOA, CAROLINA FITTIPALDI; QUINET, ANNABEL; HECHT, FABIO; KAJITANI, GUSTAVO S.; MILITO, CRISTIANE BEDRAN; CARVALHO, DENISE P.; MARTINS MENCK, CARLOS FREDERICO. DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018. (14/15982-6, 13/21075-9)

VASCONCELOS, RENATA PRADO; PEIXOTO, MILENA SIMOES; DE OLIVEIRA, KECIANY ALVES; FREITAS FERREIRA, ANDREA CLAUDIA; COELHO-DE-SOUZA, ANDRELINA NORONHA; CARVALHO, DENISE P.; DE OLIVEIRA, ARICLECIO CUNHA; FORTUNATO, RODRIGO S.. Sex differences in subcutaneous adipose tissue redox homeostasis and inflammation markers in control and high-fat diet fed rats. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 44, n. 7, p. 720-726, JUL 2019. (14/15982-6, 13/21075-9)

LERNER, LETICIA K.; FRANCISCO, GUILHERME; SOLTYS, DANIELA T.; ROCHA, CLARISSA R. R.; QUINET, ANNABEL; VESSONI, ALEXANDRE T.; CASTRO, LIGIA P.; DAVID, TAYNAH I. P.; BUSTOS, SILVINA O.; STRAUSS, BRYAN E.; et al. Predominant role of DNA polymerase eta and p53-dependent translesion synthesis in the survival of ultraviolet-irradiated human cells. Nucleic Acids Research, v. 45, n. 3, p. 1270-1280, FEB 2017. (14/15982-6, 13/21075-9)

PEDROSO, JOSE LUIZ; MUNFORD, VERIDIANA; BASTOS, ANDRE UCHIMURA; DE CASTRO, LIGIA PEREIRA; MARUSSI, VICTOR HUGO ROCHA; SILVA, GISELE SAMPAIO; ARITA, JULIANA HARUMI; MENCK, CARLOS F. M.; BARSOTTINI, ORLANDO G.. LMNB1 mutation causes cerebellar involvement and a genome instability defect. JOURNAL OF THE NEUROLOGICAL SCIENCES, v. 379, p. 249-252, AUG 15 2017. (14/15982-6, 13/21075-9)

FORTUNATO, RODRIGO S.; GOMES, LUCIANA R.; MUNFORD, VERIDIANA; PESSOA, CAROLINA FITTIPALDI; QUINET, ANNABEL; HECHT, FABIO; KAJITANI, GUSTAVO S.; MILITO, CRISTIANE BEDRAN; CARVALHO, DENISE P.; MARTINS MENCK, CARLOS FREDERICO. DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2018, p. 9-pg., 2018-01-01. (13/21075-9, 14/15982-6)

QUINET, ANNABEL; LERNER, LETICIA K.; MARTINS, DAVI J.; MENCK, CARLOS F. M.. Filling gaps in translesion DNA synthesis in human cells. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, p. 16-pg., 2018-12-01. (14/15982-6, 13/21075-9)