Identification, oxacillinase characterization and molecular typing of Acinetobacter calcoaceticus-A. baumannii complex clinical isolates from the State of São Paulo, Brazil

• Grant number: 13/12055-4
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2014 - January 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Carlos Henrique Camargo
• Grantee: Carlos Henrique Camargo
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Doroti de Oliveira Garcia ; Eliete Caló Romero ; Luis Fernando dos Santos

Abstract

Healthcare associated infections lead to increase of morbity and mortality, and can reflect in the costs of patients' management. Acinetobacter species, particularly those included in the Acinetobacter calcoaceticus-A. baumannii (ACB) Complex, are associated with several kinds of nosocomial infections, and represent an important treat to infection control. They possess a large number of resistance mechanisms against virtually all available antimicrobial agents, including the carbapenems, which had been the longstanding last-line agents for treating Acinetobacter infections. The main mechanism associated with carbapenem resistance is the beta-lactamase production of OXA enzymes. A global increase in carbapenem-resistant Acinetobacter has been reported. Polymyxin has presented high activity against Acinetobacter isolates although some resistant-isolates have had reported, including in Brazil, which lead to need of monitoring this resistance phenotype. Elucidation of clonal relationship of circulating Acinetobacter strains is desirable to monitor the spread and implement measures for controlling their dissemination. Phylogenetic analyzes, in addition, can provide information regarding the evolution and features that contribute to persistence and spread of successful strains. The aim of this study is to provide the characterization of circulating strains isolated from clinical specimens of hospitalized patients across the State of São Paulo, identifying the species, determining the antimicrobial susceptibility and defining the clonal and phylogenetic relatedness among them. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE VASCONCELLOS, FRANCIELLI MAHNIC; TIBA CASAS, MONIQUE RIBEIRO; BRISOLLA TAVARES, LAIS CALISSI; GARCIA, DOROTI DE OLIVEIRA; CAMARGO, CARLOS HENRIQUE. In vitro activity of antimicrobial agents against multidrug- and extensively drug-resistant Acinetobacter baumannii. Journal of Medical Microbiology, v. 66, n. 1, p. 98-102, JAN 2017. (13/12055-4)

DE VASCONCELLOS, FRANCIELLI MAHNIC; TIBA-CASAS, MONIQUE RIBEIRO; BRISOLLA TAVARES, LAIS CALISSI; DE SOUZA, WILLIAM VAZ; GARCIA, DOROTI DE OLIVEIRA; CAMARGO, CARLOS HENRIQUE. Evaluation of a new trilocus sequence-based multiplex-PCR to detect major Acinetobacter baumannii clonal complexes circulating in Brazil. INFECTION GENETICS AND EVOLUTION, v. 54, p. 4-6, OCT 2017. (15/13179-4, 13/12055-4)

CAMARGO, CARLOS HENRIQUE; TIBA, MONIQUE RIBEIRO; SAES, MARTA REGINA; DE VASCONCELLOS, FRANCIELLI MAHNIC; DOS SANTOS, LUIS FERNANDO; ROMERO, ELIETE CALO; GARCIA, DOROTI DE OLIVEIRA. Population Structure Analysis of Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates from Brazil Reveals Predominance of Clonal Complexes 1, 15, and 79. Antimicrobial Agents and Chemotherapy, v. 60, n. 4, p. 2545-2547, APR 2016. (13/12055-4, 14/11380-1)