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Analysis of anti-Leishmania and anti-Tripanosoma potencials of oleanolic and ursolic acids

Grant number: 13/16297-2
Support type:Regular Research Grants
Duration: February 01, 2014 - January 31, 2016
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Luiz Felipe Domingues Passero
Grantee:Luiz Felipe Domingues Passero
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:João Henrique Ghilardi Lago ; Marcia Dalastra Laurenti

Abstract

Recently, the leishmanicidal effect of different natural compounds which were purified from leaves of Baccharis uncinella was evaluated. One of them, enriched with oleanolic and ursolic acids, showed a high potencial against promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and L. (Viannia) braziliensis, without toxicity for cells and animals. To continue and increase the scope of our study, the aim of this project is to evaluate the antiparasitic potential of purified oleanolic and ursolic acids. Thus, hamsters will be infected with L. (L.) chagasi and BALB/c mice with L. (L.) amazonensis and Trypanosoma brucei. These animals will be treated with each triterpene using different doses, as well as respective reference drugs. After treatment, the parasite load in different organs will be quantified and the production or expression of IL-4, IL-10, IFN-g, TNF-a and IL-12 will be evaluated in the lymphatic organs. The level of IgG1 and IgG2 also will be evaluated. In addition, the duplication of genes associated to the resistance of drugs will be measured in extra and intracellular protozoa, which were incubated with triterpenes and standard drugs. This project aims to find new target or prototype molecules addressed to the therapeutical of neglected diseases caused by pathogenic protozoa. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JESUS, JESSICA A.; FRAGOSO, THAIS N.; YAMAMOTO, EDUARDO S.; LAURENTI, MARCIA D.; SILVA, MARCELO S.; FERREIRA, AUREA F.; LAGO, JOAO HENRIQUE G.; GOMES, GABRIELA S.; PASSERO, LUIZ FELIPE D. Therapeutic effect of ursolic acid in experimental visceral leishmaniasis. INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE, v. 7, n. 1, p. 1-11, APR 2017. Web of Science Citations: 15.
DE SOUSA, CAROLINA BRUNO; GANGADHAR, KATKAM N.; MORAIS, THIAGO R.; CONSERVA, GEANNE A. A.; VIZETTO-DUARTE, CATARINA; PEREIRA, HUGO; LAURENTI, MARCIA D.; CAMPINO, LENEA; LEVY, DEBORA; UEMI, MIRIAM; BARREIRA, LUISA; CUSTODIO, LUISA; PASSERO, LUIZ FELIPE D.; LAGO, JOAO HENRIQUE G.; VARELA, JOAO. Antileishmanial activity of meroditerpenoids from the macroalgae Cystoseira baccata. Experimental Parasitology, v. 174, p. 1-9, MAR 2017. Web of Science Citations: 12.
YAMAMOTO, EDUARDO S.; CAMPOS, BRUNO L. S.; JESUS, JESSICA A.; LAURENTI, MARCIA D.; RIBEIRO, SUSAN P.; KALLAS, ESPER G.; RAFAEL-FERNANDES, MARIANA; SANTOS-GOMES, GABRIELA; SILVA, MARCELO S.; SESSA, DEBORAH P.; LAGO, JOAO H. G.; LEVY, DEBORA; PASSERO, LUIZ F. D. The Effect of Ursolic Acid on Leishmania (Leishmania) amazonensis Is Related to Programed Cell Death and Presents Therapeutic Potential in Experimental Cutaneous Leishmaniasis. PLoS One, v. 10, n. 12 DEC 16 2015. Web of Science Citations: 18.
JESUS, JESSICA A.; LAGO, JOAO HENRIQUE G.; LAURENTI, MARCIA D.; YAMAMOTO, EDUARDO S.; PASSERO, LUIZ FELIPE D. Antimicrobial Activity of Oleanolic and Ursolic Acids: An Update. Evidence-based Complementary and Alternative Medicine, 2015. Web of Science Citations: 19.

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