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EMU: acquisition of a Raman Spectroscopy microscopy system for the molecular biology and pharmacology institute of the UNIFESP

Grant number: 13/08154-7
Support type:Multi-user Equipment Program
Duration: March 01, 2014 - February 29, 2016
Field of knowledge:Biological Sciences - Biology
Principal Investigator:Helena Bonciani Nader
Grantee:Helena Bonciani Nader
Home Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). São Paulo , SP, Brazil
Assoc. researchers:Luiz Juliano Neto
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Tipo de equipamento:Caracterização de Materiais - Imageamento - Infravermelho, Raman (fNIRS)
Fabricante: Fabricante não informado
Modelo: Modelo não informado

Abstract

Confocal microscopy is a type of fluorescence microscopy, which has had tremendous advancement and technological development in recent years, especially with regard to coupling it with other biophysical and spectroscopic techniques such as CARS (Coherent anti-Stokes Raman Scattering). This methodology is based on the Raman light scattering phenomenon. The molecular characteristics generate vibrational and rotational states characteristic for each molecule. Thus, the combination of conventional confocal microscopy technique with CARS is a new method to generate 3D images based on intrinsic vibrational spectrum of various molecules. The primary advantages of this method are that the samples remain unchanged, no fluorophores are required, avoiding cytotoxic effects, and no auto fluorescence. This new approach allows the generation of real images, free from various artifacts caused by the use of fluorophores, besides being a non-destructive methodology and minimally invasive. The present proposal, aims on the acquisition of a new microscopy system that isn't available in our country. Such equipment will enable studies of cell volume solvent diffusion, lipid cellular distribution in important aspects such as cell and tissue in atherosclerosis, metabolic analysis, besides further applications in the area of nanotechnology and biofuels chemistry. The equipment will be installed at the Institute of Pharmacology and Molecular Biology (INFAR) UNIFESP in a multiuser scheme providing services to researchers from the Departments of Biophysics, Biochemistry and Pharmacology, as well as researchers from various institutions from the São Paulo state. (AU)