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Biological changes of papaya pectins with possible benefits to human health

Grant number: 12/23970-2
Support Opportunities:Research Grants - Young Investigators Grants
Duration: March 01, 2014 - February 28, 2019
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:Joao Paulo Fabi
Grantee:Joao Paulo Fabi
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated grant(s):15/01004-5 - Multi-User Equipment approved in grant 2012/23970-2: QuantStudio 7 flex Real-Time PCR system, 96-well fast, laptop (catalog #: 4485698), AP.EMU
Associated scholarship(s):18/12603-5 - Effects of treatment on intestinal cancer cells with pectins isolated from chayote, papaya and passion fruit, BP.IC
18/06274-9 - Effect of papaya cell wall treatment with pectinases in colon cancer cells, BP.IC
18/05900-3 - Effects of in vitro inhibition of TLRs 2, 3 and 4 from human colorretal cancer cells during papaya pectin treatment, BP.IC
+ associated scholarships 18/06255-4 - In vitro effect of pectins treatments from unripe and ripe papayas in colon cancer cells spheroids, BP.IC
18/06266-6 - Effect of treatments with unripe and ripe papaya fiber in a model of in vivo colon carcinogenesis, BP.IC
16/19657-8 - Effect of acidic and neutral papaya polysaccharides on colon cancer cells, BP.IC
16/10895-3 - The biological effects of modified pectins extracted from different fruits on colon cancer cells, BP.DD
15/15661-8 - Effects of cancer cells treatment with pectins from different plant types, BP.IC
14/06535-6 - Pectic depolymerization during papaya fruit riperning: solubilization in oligosaccharides, BP.IC - associated scholarships

Abstract

Fleshy fruits are considered as rich in soluble fibers, and its consumption could reduce chronic non-communicable diseases (NCDs) frequency, including some sorts of cancer. Pectin, the main polysaccharides of fleshy fruit soluble fibers, could inhibit the pro-metastatic protein galectin-3 by selective binding of pectin-derived galactans in vivo and in vitro, thus reducing cancer cells growth. Pectin could also be fermented by human intestinal microbiota, inducing growth of symbiotic bacteria and producing short chain fatty acids (SCFA), which could in turn prevent colon cancer. Because of papaya is considered a pectin-rich fruit with great amounts of pectin-derived galactans, the project has the main objective of establishing a correlation between chemical structures of papaya pectins and its probable human consumption benefits. To achieve this goal, experiments comprising cultured cells treated with papaya pectins should be performed, as well as in vitro colonic fermentation and its metagenome. Controlled enzymatic treatments of papaya pectins ("fiber food engineering") could complement a possible improvement on health parameters. The results arose from this study would open new perspectives on the development of functional foods derived from pectin-rich fruits. (AU)

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Scientific publications (22)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES, CAROLINE GIACOMELLI; DO PRADO, SAMIRA BERNARDINO RAMOS; ANDRADE, SONIA C. S.; FABI, JOAO PAULO. Systems Biology Applied to the Study of Papaya Fruit Ripening: The Influence of Ethylene on Pulp Softening. CELLS, v. 10, n. 9, . (12/23970-2, 13/07914-8, 19/11816-8)
PAESANI, CANDELA; DEGANO, ALICIA L.; INES ZALOSNIK, MARIA; FABI, JOAO PAULO; PEREZ, GABRIELA T.. Enzymatic modification of arabinoxylans from soft and hard Argentinian wheat inhibits the viability of HCT-116 cells. Food Research International, v. 147, . (19/11816-8, 13/07914-8, 12/23970-2)
RAMOS DO PRADO, SAMIRA BERNARDINO; SHIGA, TANIA MISUZU; HARAZONO, YOSUKE; HOGAN, VICTOR A.; RAZ, AVRAHAM; CARPITA, NICHOLAS C.; FABI, JOAO PAULO. Migration and proliferation of cancer cells in culture are differentially affected by molecular size of modified citrus pectin. Carbohydrate Polymers, v. 211, p. 141-151, . (15/01004-5, 13/07914-8, 12/23970-2)
PAESANI, CANDELA; SCIARINI, LORENA S.; MOIRAGHI, MALENA; SALVUCCI, EMILIANO; PRADO, SAMIRA B. R.; TERESA PEREZ, GABRIELA; FABI, JOAO PAULO. Human colonic in vitro fermentation of water-soluble arabinoxylans from hard and soft wheat alters Bifidobacterium abundance and short-chain fatty acids concentration. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 134, . (19/11816-8, 13/07914-8, 12/23970-2)
PRADO, SAMIRA B. R.; BEUKEMA, MARTIN; JERMENDI, EVA; SCHOLS, HENK A.; DE VOS, PAUL; FABI, JOAO PAULO. Pectin Interaction with Immune Receptors is Modulated by Ripening Process in Papayas. SCIENTIFIC REPORTS, v. 10, n. 1, . (19/11816-8, 13/07914-8, 12/23970-2)
FABI, JOAO PAULO; RAMOS DO PRADO, SAMIRA BERNARDINO. Fast and Furious: Ethylene-Triggered Changes in the Metabolism of Papaya Fruit During Ripening. FRONTIERS IN PLANT SCIENCE, v. 10, . (13/07914-8, 12/23970-2)
RAMOS DO PRADO, SAMIRA BERNARDINO; CASTRO-ALVES, VICTOR COSTA; FERREIRA, GABRIELLE FERNANDEZ; FABI, JOAO PAULO. Ingestion of Non-digestible Carbohydrates From Plant-Source Foods and Decreased Risk of Colorectal Cancer: A Review on the Biological Effects and the Mechanisms of Action. FRONTIERS IN NUTRITION, v. 6, . (13/07914-8, 12/23970-2)
DONADIO, JANAINA L. S.; RAMOS DO PRADO, SAMIRA BERNARDINO; ROGERO, MARCELO M.; FABI, JOAO PAULO. Effects of pectins on colorectal cancer: targeting hallmarks as a support for future clinical trials. FOOD & FUNCTION, v. 13, n. 22, p. 17-pg., . (19/11816-8, 19/18794-0, 13/07914-8, 12/23970-2)
DER AGOPIAN, ROBERTA GHEDINI; FABI, JOAO PAULO; CORDENUNSI-LYSENKO, BEATRIZ ROSANA. Metabolome and proteome of ethylene-treated papayas reveal different pathways to volatile compounds biosynthesis. Food Research International, v. 131, . (15/15661-8, 13/07914-8, 12/23970-2)
ROSALES, THIECLA KATIANE OSVALDT; FABI, JOAO PAULO. Pectin-based nanoencapsulation strategy to improve the bioavailability of bioactive compounds. International Journal of Biological Macromolecules, v. 229, p. 11-pg., . (13/07914-8, 19/11816-8, 12/23970-2)
OSVALDT ROSALES, THIECLA KATIANE; AYMOTO HASSIMOTTO, NEUZA MARIKO; LAJOLO, FRANCO MARIA; FABI, JOAO PAULO. Nanotechnology as a Tool to Mitigate the Effects of Intestinal Microbiota on Metabolization of Anthocyanins. ANTIOXIDANTS, v. 11, n. 3, p. 22-pg., . (19/11816-8, 12/23970-2, 13/07914-8)
DA SILVA, MAGNER PESSOA; ROSALES, THIECLA KATIANE OSVALDT; PEDROSA, LUCAS DE FREITAS; FABI, JOAO PAULO. Creation of a new proof-of-concept pectin/lysozyme nanocomplex as potential beta-lactose delivery matrix: Structure and thermal stability analyses. FOOD HYDROCOLLOIDS, v. 134, p. 12-pg., . (19/11816-8, 12/23970-2, 13/07914-8)
PEDROSA, LUCAS DE FREITAS; LOPES, RODRIGO GUIMARAES; FABI, JOAO PAULO. The acid and neutral fractions of pectins isolated from ripe and overripe papayas differentially affect galectin-3 inhibition and colon cancer cell growth. International Journal of Biological Macromolecules, v. 164, p. 2681-2690, . (16/19657-8, 13/07914-8, 19/11816-8, 12/23970-2)
DO NASCIMENTO, RAISSA SANSONI; PEDROSA, LUCAS DE FREITAS; DIETHELM, LUIZA TAMIE HIRATA; SOUZA, THALES; SHIGA, TANIA M.; FABI, JAO PAULO. The purification of pectin from commercial fruit flours results in a jaboticaba fraction that inhibits galectin-3 and colon cancer cell growth. Food Research International, v. 137, . (13/07914-8, 12/23970-2, 19/11816-8)
RAMOS DO PRADO, SAMIRA BERNARDINO; SANTOS, GUSTAVO R. C.; MOURAO, PAULO A. S.; FABI, JOAO PAULO. Chelate-soluble pectin fraction from papaya pulp interacts with galectin-3 and inhibits colon cancer cell proliferation. International Journal of Biological Macromolecules, v. 126, p. 170-178, . (13/07914-8, 12/23970-2)
ROSALES, THIECLA KATIANE OSVALDT; DA SILVA, MAGNER PESSOA; LOURENCO, FELIPE REBELLO; HASSIMOTTO, NEUZA MARIKO AYMOTO; FABI, JOAO PAULO. Nanoencapsulation of anthocyanins from blackberry (Rubus spp.) through pectin and lysozyme self-assembling. FOOD HYDROCOLLOIDS, v. 114, . (19/11816-8, 13/07914-8, 12/23970-2)
PRADO, SAMIRA BERNARDINO RAMOS DO; MINGUZZI, BEATRIZ TOLEDO; HOFFMANN, CHRISTIAN; FABI, JOAO PAULO. Modulation of human gut microbiota by dietary fibers from unripe and ripe papayas: Distinct polysaccharide degradation using a colonic in vitro fermentation model. Food Chemistry, v. 348, . (19/11816-8, 12/23970-2, 13/07914-8)
RAMOS DO PRADO, SAMIRA BERNARDINO; FERREIRA, GABRIELLE FERNANDEZ; HARAZONO, YOSUKE; SHIGA, TANIA MISUZU; RAZ, AVRAHAM; CARPITA, NICHOLAS C.; FABI, JOAO PAULO. Ripening-induced chemical modifications of papaya pectin inhibit cancer cell proliferation. SCIENTIFIC REPORTS, v. 7, . (15/15661-8, 15/01004-5, 13/07914-8, 12/23970-2)
PEDROSA, LUCAS DE FREITAS; RAZ, AVRAHAM; FABI, JOAO PAULO. The Complex Biological Effects of Pectin: Galectin-3 Targeting as Potential Human Health Improvement?. BIOMOLECULES, v. 12, n. 2, p. 32-pg., . (20/08063-5, 16/19657-8, 12/23970-2, 19/11816-8, 13/07914-8)
ROSALES, THIECLA KATIANE OSVALDT; FABI, JOAO PAULO. Nanoencapsulated anthocyanin as a functional ingredient: Technological application and future perspectives. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 218, p. 16-pg., . (13/07914-8, 19/11816-8, 12/23970-2)
ROSALES, THIECLA KATIANE OSVALDT; PEDROSA, LUCAS DE FREITAS; NASCIMENTO, KAREN REBOUCAS; FIOROTO, ALEXANDRE MINAMI; TONIAZZO, TAISE; TADINI, CARMEN CECILIA; PURGATTO, EDUARDO; HASSIMOTTO, NEUZA MARIKO AYMOTO; FABI, JOAO PAULO. Nanoencapsulated anthocyanins: A new technological approach to increase physical-chemical stability and bioaccessibility. FOOD HYDROCOLLOIDS, v. 139, p. 18-pg., . (13/07914-8, 19/11816-8, 12/23970-2)
DO PRADO, SAMIRA B. R.; MELFI, PAULO R.; CASTRO-ALVES, VICTOR C.; BROETTO, SABRINA G.; ARAUJO, ELIAS S.; DO NASCIMENTO, JOAO R. O.; FABI, JOAO P.. Physiological Degradation of Pectin in Papaya Cell Walls: Release of Long Chains Galacturonans Derived from Insoluble Fractions during Postharvest Fruit Ripening. FRONTIERS IN PLANT SCIENCE, v. 7, . (14/06535-6, 13/07914-8, 12/23970-2)

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Filed patent(s) as a result of this research project

PROCESSO PARA EXTRAÇÃO E ISOLAMENTO DE FIBRAS ALIMENTARES DE MAMÕES RICAS EM PECTINAS E USO DAS MESMAS BR1020180747860 - Universidade de São Paulo (USP) . JOÃO PAULO FABI / SAMIRA BERNARDINO RAMOS DO PRADO / TÂNIA MISUZU SHIGA - November 2018, 29