Detection of arbovirus and other RNA viruses by real time RT-PCR and study of the viroma

• Grant number: 13/14929-1
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2014
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Luiz Tadeu Moraes Figueiredo
• Grantee: Luiz Tadeu Moraes Figueiredo
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Viruses transmitted by arthropods (arboviruses) are responsible for serious emerging and reemerging diseases in different areas of the world, especially in tropical and subtropical regions. Among the arboviruses Flaviviridae, Togaviridae, Bunyaviridae and Rhabdoviridae can cause clinical manifestations ranging from asymptomatic infection, acute febrile illness, hemorrhagic fevers and encephalitis. This research project aims to detect arboviruses and other RNA viruses in serum samples from febrile patients as well as arthropods. For this, we intend to develop techniques of RT-real time PCR using SYBR Green for detection and quantification of the genome of arboviruses. Besides, considering that many viruses cannot be detected by conventional methods, we are also going to use nucleotide sequencers of new generation for description of the viroma of the samples. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; CARRERA, JEAN PAUL; DE ARAUJO, JANSEN; CARDOSO, JEDSON FERREIRA; DE CARVALHO, CRISTIANO; DURIGON, EDISON LUIZ; QUEIROZ, LUZIA HELENA; FARIA, NUNO RODRIGUES; MURCIA, PABLO R.; et al. Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism. INFECTION GENETICS AND EVOLUTION, v. 95, NOV 2021. (15/05778-5, 19/24251-9, 13/14929-1, 18/09383-3, 17/13981-0, 18/14389-0)

DE SOUZA, WILLIAM MARCIE; FUMAGALLI, MARCILIO JORGE; TORRES CARRASCO, ADRIANO DE OLIVEIRA; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; SEKI, MEIRE CHRISTINA; GHELLER, JANAINA MENEGAZZO; DAFFRE, SIRLEI; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; et al. Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil. SCIENTIFIC REPORTS, v. 8, NOV 5 2018. (16/01414-1, 14/02438-6, 15/05778-5, 13/14929-1, 12/24150-9, 17/13981-0, 14/20851-8)

FUMAGALLI, MARCILIO JORGE; DE SOUZA, WILLIAM MARCIEL; DE ARAUJO, JANSEN; MODHA, SEJAL; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Krykfeie dicistrovirus: A novel dicistrovirus in velvety free-tailed bats from Brazil. INFECTION GENETICS AND EVOLUTION, v. 75, NOV 2019. (12/24150-9, 17/13981-0, 16/01414-1, 13/14929-1, 14/20851-8, 15/05778-5)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; MODHA, SEJAL; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. A Novel Hepacivirus in Wild Rodents from South America. Viruses-Basel, v. 11, n. 3, MAR 24 2019. (12/24150-9, 17/13981-0, 16/01414-1, 13/14929-1, 16/02568-2, 11/06810-9, 08/06411-4, 11/22663-6, 15/05778-5)

DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Novel orthohepeviruses in wild rodents from Sao Paulo State, Brazil. VIROLOGY, v. 519, p. 12-16, JUN 2018. (16/02568-2, 12/24150-9, 17/13981-0, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 11/06810-9, 08/06411-4, 11/22663-6)

ALINE LAVADO TOLARDO; WILLIAM MARCIEL DE SOUZA; MARILIA FARIGNOLI ROMEIRO; LUIZ CARLOS VIEIRA; LUCIANO KLEBER DE SOUZA LUNA; DYANA ALVES HENRIQUES; JANSEN DE ARAUJO; CARLOS EDUARDO HASSEGAWA SIQUEIRA; TATIANA ELIAS COLOMBO; VICTOR HUGO AQUINO; et al. A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus. Memórias do Instituto Oswaldo Cruz, v. 111, n. 6, p. 385-390, 2016-06-03. (13/02256-2, 13/14929-1, 12/02836-6, 12/24150-9)

ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; HENRIQUES, DYANA ALVES; DE ARAUJO, JANSEN; HASSEGAWA SIQUEIRA, CARLOS EDUARDO; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; LOPES DA FONSECA, BENEDITO ANTONIO; et al. A real-time RT-PCR for rapid detection and quantification of mosquito-borne alphaviruses. ARCHIVES OF VIROLOGY, v. 161, n. 11, p. 3171-3177, NOV 2016. (13/02256-2, 12/02836-6, 13/14929-1, 12/24150-9, 14/20851-8)

NUNES-NETO, JOAQUIM PINTO; DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; FUMAGALLI, MARCILIOJORGE; VIEIRA, LUIZ CARLOS; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; LIMA, JULIANA ABREU; SILVA DE LIMA, CLAYTON PEREIRA; CARDOSO, JEDSON FERREIRA; et al. Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil. JOURNAL OF GENERAL VIROLOGY, v. 98, n. 4, p. 585-594, APR 2017. (12/24150-9, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 14/02438-6)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; DE ARAUJO, JANSEN; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONSALVES; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; NARDI, MARCELLO SCHIAVO; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; et al. Discovery of novel anelloviruses in small mammals expands the host range and diversity of the Anelloviridae. VIROLOGY, v. 514, p. 9-17, JAN 15 2018. (16/02568-2, 12/24150-9, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 11/06810-9, 08/06411-4, 11/22663-6)

MOTTA MAIA, FELIPE GONCALVES; DE SOUZA, WILLIAM MARCIEL; SABINO-SANTOS, JR., GILBERTO; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. A novel polyomavirus in sigmodontine rodents from So Paulo State, Brazil. ARCHIVES OF VIROLOGY, v. 163, n. 10, p. 2913-2915, OCT 2018. (12/24150-9, 17/13981-0, 16/01414-1, 13/14929-1, 16/02568-2, 11/06810-9, 08/06411-4, 11/22663-6, 15/05778-5)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; DE ARAUJO, JANSEN; OMETTO, TATIANA; MODHA, SEJAL; THOMAZELLI, LUCIANO MATSUMIYA; DURIGON, EDISON LUIS; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Discovery of novel astrovirus and calicivirus identified in ruddy turnstones in Brazil. SCIENTIFIC REPORTS, v. 9, APR 3 2019. (11/13821-7, 12/24150-9, 17/13981-0, 16/01414-1, 15/05778-5, 18/09383-3, 13/05485-2, 13/14929-1, 09/05994-9)

DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; DE ARAUJO, JANSEN; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; MORAES FIGUEIREDO, LUIZ TADEU; MURCIA, PABLO RAMIRO; GIFFORD, ROBERT JAMES. Chapparvoviruses occur in at least three vertebrate classes and have a broad biogeographic distribution. JOURNAL OF GENERAL VIROLOGY, v. 98, n. 2, p. 225-229, FEB 2017. (12/24150-9, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; MARTIN, MATHEUS CAVALHEIRO; DE ARAUJO, JANSEN; ORSI, MARIA ANGELA; SANFILIPPO, LUIZ FRANCISCO; MODHA, SEJAL; DURIGON, EDISON LUIZ; PROENCA-MODENA, JOSE LUIZ; ARNS, CLARICE WEIS; et al. Pingu virus: A new picornavirus in penguins from Antarctica. VIRUS EVOLUTION, v. 5, n. 2, JUL 2019. (17/13981-0, 12/24150-9, 16/01414-1, 13/14929-1, 14/20851-8, 15/05778-5)

DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; REIS, JR., OSVALDO; TOLARDO, ALINE LAVADO; DA SILVA, SANDRO PATROCA; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; VARELA, MARIANA; TEIXEIRA NUNES, MARCIO ROBERTO; MORAES FIGUEIREDO, LUIZ TADEU. Molecular characterization of Capim and Enseada orthobunyaviruses. INFECTION GENETICS AND EVOLUTION, v. 40, p. 47-53, JUN 2016. (14/20851-8, 12/24150-9, 15/05778-5, 13/14929-1, 14/02438-6)

ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; NOGUEIRA, MAURICIO LACERDA; MORAES FIGUEIREDO, LUIZ TADEU. Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 3, p. 279-285, MAY-JUN 2016. (13/02256-2, 12/02836-6, 14/20851-8, 12/24150-9, 13/14929-1)

DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; REIS JUNIOR, OSVALDO; TOLARDO, ALINE LAVADO; SERRAO DE ANDRADE, AMANDA ARAUJO; GONCALVES VIANEZ JUNIOR, JOAO LIDIO DA SILVA; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; TEIXEIRA NUNES, MARCIO ROBERTO; MORAES FIGUEIREDO, LUIZ TADEU. Complete genome sequence of Piry vesiculovirus. ARCHIVES OF VIROLOGY, v. 161, n. 8, p. 2325-2328, AUG 2016. (12/24150-9, 13/14929-1, 14/20851-8, 13/02256-2, 14/02438-6, 15/05778-5)

DE SOUZA, WILLIAM MARCIEL; DENNIS, TRISTAN; FUMAGALLI, MARCILIO JORGE; ARAUJO, JANSEN; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; ACRANI, GUSTAVO OLSZANSKI; TORRES CARRASCO, ADRIANO DE OLIVEIRA; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; et al. Novel Parvoviruses from Wild and Domestic Animals in Brazil Provide New Insights into Parvovirus Distribution and Diversity. Viruses-Basel, v. 10, n. 4, APR 2018. (11/13821-7, 16/02568-2, 12/24150-9, 17/13981-0, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 09/05994-9, 11/06810-9, 08/06411-4, 11/22663-6)