Effects of eugenol and dehidrodieugenol isolated from Nectandra leucantha (Laurace...
| Grant number: | 13/17944-1 |
| Support type: | Regular Research Grants |
| Duration: | May 01, 2014 - October 31, 2016 |
| Field of knowledge: | Biological Sciences - Physiology |
| Principal Investigator: | Iolanda de Fátima Lopes Calvo Tibério |
| Grantee: | Iolanda de Fátima Lopes Calvo Tibério |
| Home Institution: | Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Assoc. researchers: | Carla Máximo Prado ; Edna Aparecida Leick ; Flávia Castro Ribas de Souza ; Luciana Ritha de Cássia Rolim Barbosa Aristóteles ; Milton de Arruda Martins ; Patricia Angeli da Silva Pigati ; Renato Fraga Righetti ; Ruth Andriani Baroni ; Samantha Souza Possa |
Abstract
Th17 cytokines seem to play an important role in the pathogenesis of chronic allergic pulmonary inflammation and acute lung injury (ALI), although its exact influence is uncertain. Studies have demonstrated an influence of these cytokines in modulating inflammation or infection, and the infection is, in turn, one of the factors involved in the maintenance of the inflammation response in asthma. Many of the effects of this pathway have not been investigated in models of chronic pulmonary inflammation (asthma) associated or not with acute lung injury (ALI). In the present study will evaluate the effects of treatment with anti-IL-17 in a model of chronic allergic pulmonary inflammation and ALI induced by lipopolysaccharide (LPS). We assess lung mechanics, inflammatory response, activation of oxidative stress, extracellular matrix remodeling in the airways and in the distal lung of mice chronically exposed to ovalbumin or lung injury induced by LPS. The BALB/c mice will be sensitized to ovalbumin for 28 days and controls receive saline treatment groups receive one hour prior to exposure to ovalbumin administration of neutralizing antibody anti-IL-17 intranasal and intraperitoneal on days 22, 24, 26 and 28. The animals underwent LPA will be anesthetized with isoflurane and will receive single intratracheal instillation of LPS diluted in saline at a dose of 5mg/kg. The treatment groups will receive a single dose of anti-IL-17 hour before exposure to LPS and control groups receive saline. Twenty-four hours after the end of the experimental protocol, the animals will be subject to an assessment of lung mechanics. Then it will be made histological analyzes for numbers of CD4 +, CD8 +, dendritic cells, collagen deposition (types I, III and V), elastic, actin, decorin, byglican, lumican, fibronectin, integrins (±V²6 and TLR3) , FOXP3, CCL-11, TARC, MMP-9, MMP-12 and TIMP-1, isoprostane, cytokines (IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL -17, IFN-gamma, TNF-±, TGF-²1, NFºBand of Rho kinase activityin different groups. (AU)