Effect of low level laser therapy in experimental models of pulmonary chronic diseases

• Grant number: 12/16498-5
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: June 01, 2014
• End date: May 31, 2019
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Ana Paula Ligeiro de Oliveira
• Grantee: Ana Paula Ligeiro de Oliveira
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated researchers: João Palermo Neto ; Wothan Tavares de Lima
• Associated scholarship(s): 16/11776-8 - Low-level laser therapy effects on Th2 lymphocytes during pulmonary inflammation in chronic asthma experimental model, BP.IC; 15/23152-6 - Effect of low level laser therapy (LLLT) in an experimental model of pulmonary allergic inflammation induced house dust mite (HDM): study of dosimetry, BP.DR; 15/13486-4 - Effect of low-level laser therapy on pulmonary inflammation in an experimental model of chronic obstructive pulmonary disease (COPD) induced by cigarette smoking: laser dosimetry studies, BP.IC; 14/15806-3 - Effect of low power laser therapy (LBP) on pulmonary inflammation in an experimental model of chronic asthma: dosimetry studies, BP.IC

Abstract

Currently asthma and Chronic Obstructive Pulmonary Disease (COPD) have a high prevalence and a high economic and social cost. In this context, several experimental models have been proposed, aiming to advance knowledge about the pathophysiological processes of asthma and COPD, as well as the discovery of new therapeutic options. Therapy with Low Power Laser (LBP) is a relatively new effective therapy, with very low cost and no side effects. In this project we aim to study some parameters in animals with asthma and COPD undergoing therapy with LBP. The protocol used for the induction of asthma will be administered OVA subcutaneously (days 0 and 14) and intranasal OVA (three times / day for five weeks) or HDM intranasally (on days 0, 7, 14, 21, 28, 35 and 42). On days 43 (OVA) and 50 (HDM), animals will be sacrificed for morphologic and functional analysis of the lung. The protocol used for the induction of COPD is inhaling C57BL/6 mice for 60 days (2 times / day) with cigarette smoke. On day 61, animals will be sacrificed for morphologic and functional analysis of the lung. In both models, we will study functional and structural parameters, such as the release of cytokines and chemokines (IL-4, IL-5, IL-10, IL-13, MCP-1, TNF-±, IL-1², IL-6 and IL-10. We will also evaluate the participation of the transcription factors STAT6, GATA3 and STAT4 and NF-ºB (intact and phosphorylated), MMP-9 and MMP-12, TIMP-1 and TIMP-2 and the evaluation lung mechanics and airway hyperresponsiveness in both experimental models will be performed. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DURIGON, THOMAS STRAVINSKAS; MACKENZIE, BREANNE; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; SANTOS-DIAS, ALANA; DE ANGELIS, KATIA; MALFITANO, CHRISTIANO; DA PALMA, RENATA KELLY; GUERRA, JULIANA MORENO; DAMACENO-RODRIGUES, NILSA REGINA; CALDINI, ELIA GARCIA; et al. Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice. JOURNAL OF INNATE IMMUNITY, v. 10, n. 4, p. 279-290, 2018. (12/16498-5, 16/08280-0, 12/15165-2, 14/23196-0, 12/23305-9, 14/14604-8)

MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; et al. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2018, p. 8-pg., 2018-01-01. (12/16498-5, 14/14604-8, 15/23152-6, 15/13486-4, 12/15165-2)

BRITO, A.; SANTOS, T.; HERCULANO, K.; MIRANDA, M.; SA, A. K.; CARVALHO, J. L.; ALBERTINI, R.; CASTRO-FARIA-NETO, H.; LIGEIRO-DE-OLIVEIRA, A. P.; AIMBIRE, FLAVIO. The MAPKinase Signaling and the Stimulatory Protein-1 (Sp1) Transcription Factor Are Involved in the Phototherapy Effect on Cytokines Secretion from Human Bronchial Epithelial Cells Stimulated with Cigarette Smoke Extract. Inflammation, v. 44, n. 4, p. 1643-1661, AUG 2021. (12/16498-5)

DE BRITO, AURILEIA APARECIDA; GONCALVES SANTOS, TAWANY; HERCULANO, KARINE ZANELLA; ESTEFANO-ALVES, CINTIA; DE ALVARENGA NASCIMENTO, CRISTIANO RODRIGO; RIGONATO-OLIVEIRA, NICOLE CRISTINE; CHAVANTES, MARIA CRISTINA; AIMBIRE, FLAVIO; DA PALMA, RENATA KELLY; LIGEIRO DE OLIVEIRA, ANA PAULA. hotobiomodulation Therapy Restores IL-10 Secretion in a Murine Model of Chronic Asthma: Relevance to the Population of CD4(+)CD25(+)Foxp3(+) Cells in Lun. FRONTIERS IN IMMUNOLOGY, v. 12, FEB 2 2022. (12/16498-5)

DE BRITO, AURILEIA APARECIDA; DA SILVEIRA, ELAINE CRISTINA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; SOARES, STEPHANIE SOUZA; RODRIGUES BRANDAO-RANGEL, MAYSA ALVES; SOARES, CLARIANA RODRIGUES; SANTOS, TAWANY GONCALVES; ALVES, CINTIA ESTEFANO; HERCULANO, KARINE ZANELLA; VIEIRA, RODOLFO PAULA; et al. Low-level laser therapy attenuates lung inflammation and airway remodeling in a murine model of idiopathic pulmonary fibrosis: Relevance to cytokines secretion from lung structural cells. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 203, JAN 2020. (12/16498-5)

MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; et al. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018. (12/16498-5, 15/13486-4, 12/15165-2, 14/14604-8, 15/23152-6)

GARCIA, MONIQUE; SANTOS-DIAS, ALANA; LACERDA BACHI, ANDRE LUIS; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ANDRADE-SOUZA, ADILSON SANTOS; FERREIRA, SERGIO CESAR; JONCO AQUINO-JUNIOR, JEFFERSON COMIN; ALMEIDA, FRANCINE MARIA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; LIGEIRO OLIVEIRA, ANA PAULA; et al. Creatine supplementation impairs airway inflammation in an experimental model of asthma involving P2 x 7 receptor. European Journal of Immunology, v. 49, n. 6, p. 928-939, JUN 2019. (14/12755-9, 12/16498-5, 14/14604-8, 12/15165-2)

ALMEIDA-OLIVEIRA, A. R.; AQUINO, JR., J. C. J.; ABBASI, A.; SANTOS-DIAS, A.; OLIVEIRA, JR., M. C.; ALBERCA-CUSTODIO, R. W.; RIGONATO-OLIVEIRA, N. C.; SALLEY-DIAS, L. P.; DAMACENO-RODRIGUES, N. R.; CALDINI, E. G.; et al. Effects of Aerobic Exercise on Molecular Aspects of Asthma: Involvement of SOCS-JAK-STAT. EXERCISE IMMUNOLOGY REVIEW, v. 25, p. 98-110, 2019. (12/16498-5, 12/15165-2, 14/12755-9, 14/07500-1, 12/21519-1)

CANDEO, LARISSA CARBONERA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; BRITO, AURILEIA APARECIDA; MARCOS, RODRIGO LABAT; FRANCA, CRISTIANE MIRANDA; SANTOS FERNANDES, KRISTIANNE PORTA; MESQUITA-FERRARI, RAQUEL AGNELLI; BUSSADORI, SANDRA KALIL; VIEIRA, RODOLFO PAULA; LINO-DOS-SANTOS-FRANCO, ADRIANA; et al. Effects of periodontitis on the development of asthma: The role of photodynamic therapy. PLoS One, v. 12, n. 11, NOV 16 2017. (15/20535-1, 12/16498-5, 15/23152-6, 15/00830-9)

DA SILVA, CRISTIANE MIRANDA; LEAL, MAYARA PERES; BROCHETTI, ROBSON ALEXANDRE; BRAGA, TARCIO; VITORETTI, LUANA BEATRIZ; SARAIVA CAMARA, NIELS OLSEN; DAMAZO, AMILCAR SABINO; LIGEIRO-DE-OLIVEIRA, ANA PAULA; CHAVANTES, MARIA CRISTINA; LINO-DOS-SANTOS-FRANCO, ADRIANA. Low Level Laser Therapy Reduces the Development of Lung Inflammation Induced by Formaldehyde Exposure. PLoS One, v. 10, n. 11, NOV 16 2015. (11/51711-9, 12/16498-5, 15/00830-9)

SCHATZMANN PERON, JEAN PIERRE; DE BRITO, AURILEIA APARECIDA; PELATTI, MAYRA; BRANDAO, WESLEY NOGUEIRA; VITORETTI, LUANA BEATRIZ; GREIFFO, FLAVIA REGINA; DA SILVEIRA, ELAINE CRISTINA; OLIVEIRA-JUNIOR, MANUEL CARNEIRO; MALUF, MARIANGELA; EVANGELISTA, LUCILA; et al. Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke-Induced COPD in C57BL/6 mice. PLoS One, v. 10, n. 8, AUG 31 2015. (12/16498-5, 11/18703-2)