Role of physical exercise in regulation of rock protein and the effects on insulin signaling in skeletal muscle and liver in obese mice

• Grant number: 13/21491-2
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2014
• End date: June 30, 2016
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: José Rodrigo Pauli
• Grantee: José Rodrigo Pauli
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Adelino Sanchez Ramos da Silva ; Dennys Esper Corrêa Cintra ; Eduardo Rochete Ropelle

Abstract

Obesity is a disease that is growing at an alarming rate and is associated with insulin resistance and development of chronic diseases such as cardiovascular diseases, some cancers and type 2 diabetes mellitus. Therefore, understanding the molecular mechanisms related to insulin signaling becomes essential to reduce to or reverse the progression of this alarming epidemiological reality. Insulin resistance can be installed in muscle and also in hepatic tissue, contributing substantially to initially alteration in postprandial glycaemia and later in fasting glycaemia. Moreover, the regular practice of physical exercise can improve insulin action in skeletal muscle increasing glucose uptake and in the liver reducing the formation of new glucose (gluconeogenesis). However, the effects of physical exercise on the insulin molecular pathway are not very well known. Recently, a new protein has been classified as a mediator of insulin signaling by enhancing its effects, this new protein is Rho-kinase (Rock) that acts on peripheral tissues (skeletal muscle, hepatic and adipose tissues) and in the hypothalamus, contributing to 50% insulin action in the intracellular environment. On the other hand, experimental animals that had the deletion or inhibition of Rock became insulin resistant. Seen this, studies about this new protein are necessary to better understand this complex signaling. Up to now no one study was performed to evaluate the physical exercise effects on protein expression of Rock, this project aims to investigate the role of physical exercise in acute and chronic regulation of protein Rock and its effects on signaling insulin in liver and skeletal muscle of obese mice. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUNOZ, VITOR R.; GASPAR, RAFAEL C.; SEVERINO, MATHEUS B.; MACEDO, ANA P. A.; SIMABUCO, FERNANDO M.; ROPELLE, EDUARDO R.; CINTRA, DENNYS E.; DA SILVA, ADELINO S. R.; KIM, YOUNG-BUM; PAULI, JOSE RODRIGO. Exercise Counterbalances Rho/ROCK2 Signaling Impairment in the Skeletal Muscle and Ameliorates Insulin Sensitivity in Obese Mice. FRONTIERS IN IMMUNOLOGY, v. 12, JUN 21 2021. (16/18488-8, 13/21491-2, 15/26000-2)

RODRIGUES, BARBARA DE A.; MUNOZ, VITOR R.; KUGA, GABRIEL K.; GASPAR, RAFAEL C.; NAKANDAKARI, SUSANA C. B. R.; CRISOL, BARBARA M.; BOTEZELLI, JOSE D.; PAULI, LUCIANA S. S.; DA SILVA, ADELINO S. R.; DE MOURA, LEANDRO P.; et al. Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 11, OCT 9 2017. (13/21491-2, 16/18488-8, 13/25512-4)

MOURA, LEANDRO P.; MUNOZ, VITOR R.; LIMA, INES S.; GASPAR, RAFAEL C.; VEIGA, CAMILLA B.; ROPELLE, EDUARDO R.; CINTRA, DENNYS E.; SILVA, ADELINO S. R.; BOTEZELLI, JOSE DIEGO; MEKARY, RANIA A.; et al. Physical Exercise Increases Glucose Uptake in Skeletal Muscle of Obese Mice Through Rho-Kinase Metabolism. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 48, n. 5, p. 1-pg., 2016-05-01. (13/21491-2, 13/00554-6)

BOTEZELLI, JOSE D.; COOPE, ANDRESSA; GHEZZI, ANA C.; CAMBRI, LUCIELI T.; MOURA, LEANDRO P.; SCARIOT, PEDRO P. M.; GASPAR, RODRIGO STELLZER; MEKARY, RANIA A.; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO. Strength Training Prevents Hyperinsulinemia, Insulin Resistance, and Inflammation Independent of Weight Loss in Fructose-Fed Animals. SCIENTIFIC REPORTS, v. 6, AUG 4 2016. (13/21491-2, 13/20293-2, 16/14380-8)

RODRIGUES, BARBARA DE ALMEIRA; SOUZA PAULI, LUCIANA SANTOS; DE SOUZA, CLAUDIO TEODORO; RAMOS DA SILVA, ADELINO SANCHEZ; CORREA CINTRA, DENNYS ESPER; MARINHO, RODOLFO; DE MOURA, LEANDRO PEREIRA; CHIARREOTTO ROPELLE, ELOIZE CRISTINA; BOTEZELLI, JOSE DIEGO; ROPELLE, EDUARDO ROCHETE; et al. Acute Exercise Decreases Tribbles Homolog 3 Protein Levels in the Hypothalamus of Obese Rats. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 47, n. 8, p. 1613-1623, AUG 2015. (13/21491-2, 13/25512-4)

MUNOZ, VITOR ROSETTO; GASPAR, RAFAEL CALAIS; CRISOL, BARBARA MOREIRA; FORMIGARI, GUILHERME PEDRON; SANT'ANA, MARCELLA RAMOS; BOTEZELLI, JOSE DIEGO; GASPAR, RODRIGO STELLZER; DA SILVA, ADELINO S. R.; CINTRA, DENNYS ESPER; DE MOURA, LEANDRO PEREIRA; et al. Physical exercise reduces pyruvate carboxylase (PCB) and contributes to hyperglycemia reduction in obese mice. Journal of Physiological Sciences, v. 68, n. 4, p. 493-501, JUL 2018. (13/21491-2, 16/18488-8)

GASPAR, RAFAEL C.; MUNOZ, VITOR R.; MEKARY, RANIA A.; MOURA, LEANDRO P.; SANT'ANA, MARCELLA R.; QUARESMA, PAULA; PRADA, PATRICIA O.; MARINHO, RODOLFO; PAULI, JOSE RODRIGO. Exercise Reduces Hepatic Gluconeogenesis in Obese and Insulin Resistant Animals Through CLK2 Protein (Cdc2-Like Kinase). MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 48, n. 5, p. 1-pg., 2016-05-01. (13/21491-2)