Pre-clinical studies in the mdx mouse: metabolomics, biomarkers and omega-3 therapy

• Grant number: 14/04782-6
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2014 - July 31, 2017
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Maria Julia Marques
• Grantee: Maria Julia Marques
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Elaine Minatel ; Humberto Santo Neto

Abstract

Duchenne muscle dystrophy (DMD) is a progressive degenerative disease that affects skeletal and cardiac muscles, leading to death by the age of 25 due to cardiorespiratory failure. Although there is no cure for this disease, recent gene and drug therapies are being able to slow down the progression of the disease, improving life quality and duration. The need for new biomarkers to better follow the progression of the disease and to better evaluate the effects of these new therapies is one of the main points of investigation worldwide in DMD research. We previously demonstrated positive effects of omega-3 therapy in the mdx mice model of DMD, during the early stages of the disease. These promising results motivated us to go further on omega-3 mechanisms and effects. In the present study, by using modern techniques such as metabolomics, we will search for new potential biomarkers of dystrophy in omega-3 treated-mdx mice. We aim to offer data that can support omega-3 therapy in DMD clinical trials. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAURICIO, ADRIANA FOGAGNOLO; PEREIRA, JULIANO ALVES; NETO, HUMBERTO SANTO; MARQUES, MARIA JULIA. Effects of fish oil containing eicosapentaenoic acid and docosahexaenoic acid on dystrophic mdx mice hearts at later stages of dystrophy. NUTRITION, v. 32, n. 7-8, p. 855-862, JUL-AUG 2016. (12/13577-1, 14/04782-6)

MAURICIO, ADRIANA FOGAGNOLO; DE CARVALHO, SAMARA CAMACARI; SANTO NETO, HUMBERTO; MARQUES, MARIA JULIA. Effects of dietary omega-3 on dystrophic cardiac and diaphragm muscles as evaluated by H-1 magnetic resonance spectroscopy: Metabolic profile and calcium-related proteins. CLINICAL NUTRITION ESPEN, v. 20, p. 60-67, AUG 2017. (12/13577-1, 12/15492-3, 14/04782-6)

CHAGAS BARBIN, ISABEL CRISTINA; PEREIRA, JULIANO ALVES; ROVERE, MATHEUS BERSAN; MOREIRA, DRIELEN DE OLIVEIRA; MARQUES, MARIA JULIA; SANTO NETO, HUMBERTO. Diaphragm degeneration and cardiac structure in mdx mouse: potential clinical implications for Duchenne muscular dystrophy. Journal of Anatomy, v. 228, n. 5, p. 784-791, MAY 2016. (14/04782-6)

PEREIRA, JULIANO ALVES; MARQUES, MARIA JULIA; SANTO NETO, HUMBERTO. Co-administration of deflazacort and doxycycline: a potential pharmacotherapy for Duchenne muscular dystrophy. Clinical and Experimental Pharmacology and Physiology, v. 42, n. 7, p. 788-794, JUL 2015. (14/04782-6)

DE CARVALHO, SAMARA CAMACARI; MATSUMURA, CINTIA YURI; NETO, HUMBERTO SANTO; MARQUES, MARIA JULIA. Identification of plasma interleukins as biomarkers for deflazacort and omega-3 based Duchenne muscular dystrophy therapy. CYTOKINE, v. 102, p. 55-61, FEB 2018. (12/15492-3, 14/04782-6, 13/00312-2)

DE CARVALHO, SAMARA CAMACARI; HINDI, SAJEDAH M.; KUMAR, ASHOK; MARQUES, MARIA JULIA. Effects of omega-3 on matrix metalloproteinase-9, myoblast transplantation and satellite cell activation in dystrophin-deficient muscle fibers. Cell and Tissue Research, v. 369, n. 3, p. 591-602, SEP 2017. (14/04782-6)

MOREIRA, DRIELEN DE OLIVEIRA; NETO, HUMBERTO SANTO; MARQUES, MARIA JULIA. P2Y(2) purinergic receptors are highly expressed in cardiac and diaphragm muscles of mdx mice, and their expression is decreased by suramin. MUSCLE & NERVE, v. 55, n. 1, p. 116-121, JAN 2017. (12/03498-7, 14/04782-6)

APOLINARIO, LETICIA MONTANHOLI; DE CARVALHO, SAMARA CAMACARI; NETO, HUMBERTO SANTO; MARQUES, MARIA JULIA. Long-Term Therapy With Omega-3 Ameliorates Myonecrosis and Benefits Skeletal Muscle Regeneration in Mdx Mice. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 298, n. 9, p. 1589-1596, SEP 2015. (10/14750-3, 11/02474-4, 10/13412-7, 14/04782-6)

MARANHAO, JULIANA BARROS; MOREIRA, DRIELEN DE OLIVEIRA; MAURICIO, ADRIANA FOGAGNOLO; DE CARVALHO, SAMARA CAMACARI; FERRETTI, RENATO; PEREIRA, JULIANO ALVES; SANTO NETO, HUMBERTO; MARQUES, MARIA JULIA. Changes in calsequestrin, TNF-alpha, TGF-beta and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles. International Journal of Experimental Pathology, v. 96, n. 5, p. 285-293, OCT 2015. (12/03498-7, 12/13577-1, 12/15492-3, 14/04782-6)

FINKLER, JULIA M. G.; DE CARVALHO, SAMARA C.; NETO, HUMBERTO SANTO; MARQUES, MARIA J.. Cardiac and skeletal muscle changes associated with rosuvastatin therapy in dystrophic mdx mice. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 303, n. 8, DEC 2019. (12/15492-3, 17/24051-4, 14/04782-6)

MARQUES, MARIA JULIA. A Potential New Therapeutic Approach to DMD: PKC Theta Inhibition. EBIOMEDICINE, v. 16, p. 2-pg., 2017-02-01. (14/04782-6)