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The repair of tympanic perforations with stem cells derived from fat of human male donors

Grant number: 13/21286-0
Support Opportunities:Regular Research Grants
Duration: July 01, 2014 - June 30, 2016
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Miguel Angelo Hyppolito
Grantee:Miguel Angelo Hyppolito
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated researchers:Marco Andrey Cipriani Frade


Tympanic membrane may have its anatomical structure compromised, evolving with drilling that may be permanent or temporary in the middle ear diseases and trauma. There is an interest to simplify the microsurgical procedures for the reconstruction of the tympanic membrane. Substances such as growth factors, hyaluronic acid, keratinocytes culture, insulin and the mesenquimal stem cells have been used with relative success in closing perforations of the tympanic membrane. The adipose tissue is a rich source and easy access as pluripotent stem cells, with precursor cells of adipocytes in varied degree of differentiation, vascular cells and stromal cells of support that can differentiate in vitro, in cells, condrogenics, adipogenics, osteogenic and myogenic. The stromal vascular fraction of adipose tissue can also generate endothelial progenitor cells or muscle. Objectives. To study the effect of the application of adipose Stem Cells (CTA) in the regeneration of the tympanic membrane (TM) injured. Material and Methods. 45 Animals will be divided in Control group (SHAM) with 30 left ears and traumatic perforations in tense pars of MT, with 3 days 5 days and 7 days of drilling. Group CTA, with perforations of MT treated with the CTA, having as support to fibrin glue, once, by completing the drilling, with 3 days, 5 days and 7 days of drilling. Group TASA (abdominal subcutaneous adipose tissue), with abdominal fat mouse settles into drilling, with 3 days, 5 days and 7 days of drilling. The animals will be assessed for the time of the auditory threshold and closing of perforation and the samples of tympanic bulla with the tympanic membrane will be evaluated by conventional histology (hematoxylin and eosin and Picrosirus red), immunohistochemistry and in situ hybridization (FISH). (AU)

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