Anti-diabetogenic, antioxidant / antiinflammatory / antiapoptotic effects of aqueous extract of Passiflora alata and components vitexin, Isoorientin, rutin and catechin

• Grant number: 13/25256-8
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2014 - August 31, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Ricardo de Lima Zollner
• Grantee: Ricardo de Lima Zollner
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Laura Maria Molina Meletti ; Luis Gustavo Romani Fernandes

Abstract

The search for new drugs from medicinal plants has motivated the research for alternative substances with potential anti-inflammatory that could aid in the treatment of inflammatory diseases, as in the case of type 1 diabetes. In the previous study, (International Immunopharmacology - title: Antioxidant and anti-diabetic potential of Passiflora alata Curtis aqueous leaves extract in type 1 diabetes mellitus (NOD-mice, in press, fapesp: 2011/06559-4) we have found that substances in the aqueous extract of leaves of Passiflora alata Curtis was able to decrease the incidence of the disease modulating the inflammatory mechanisms by decreasing the cellular infiltrate, apoptosis, oxidative stress, cellular infiltration, in the pancreatic islets. Moreover, we found the preserved gluthatione (liver and kidney) and insulin in this animal model. Additional properties, the decrease of the cell cycle regulatory proteins expression, such as CDK6, and increasing insulin levels in the pancreatic islets. CDK6 expression in infiltrative cells in the pancreatic islets and its reduction in the treated group suggest that flavonoids found in the leaves of P. alata have an anti-proliferative action and may have contributed to the inhibitory protein expression and subsequent cell cycle arrest of activated inflammatory cells (submitted). Thus, this study aims to evaluate the anti-inflammatory effects and the mechanisms by which the aqueous extract and the components vitexin, isorientin, catechin and rutin in vitro may have a regulatory property in cell cycle regulation, cell proliferation, oxidative stress and apoptosis in pancreatic islets submitted to mixed cell culture with activated T cell, as well as investing in the development of new drugs with the potential to interfere with the anti-inflammatory process and development of type1 diabetes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SCHUMACHER, NAYARA SIMON GONZALEZ; FERNANDES, LUS GUSTAVO ROMANI; ZOLLNER, RICARDO DE LIMA. Aqueous extract of Passiflora alata leaves modulates in vitro the indoleamine 2,3-dioxygenase (IDO) and CD86 expression in bone marrow-derived professional antigen-presenting cells polarizing NOD mice T cells to a Treg profile. CYTOKINE, v. 152, p. 9-pg., 2022-04-01. (13/25256-8)

FIGUEIREDO, D.; COLOMEU, TALITA CRISTINA; GONZALEZ SCHUMACHER, NAYARA SIMON; STIVANIN-SILVA, L. G.; BETIM CAZARIN, CINTHIA BAU; MOLINA MELETTI, LAURA MARIA; ROMANI FERNANDES, LUIS GUSTAVO; PRADO, MARCELO ALEXANDRE; ZOLLNER, R. L.. Aqueous leaf extract of Passiflora alata Curtis promotes antioxidant and anti-inflammatory effects and consequently preservation of NOD mice beta cells (non-obese diabetic). International Immunopharmacology, v. 35, p. 127-136, JUN 2016. (11/06559-4, 13/25256-8, 11/15763-4)