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Role of integrins on the osteoblast differentiation of mesenchymal stem cells induced by titanium surfaces with micro and nanotopography

Grant number: 13/05181-3
Support Opportunities:Regular Research Grants
Duration: December 01, 2014 - November 30, 2016
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Márcio Mateus Beloti
Grantee:Márcio Mateus Beloti
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated researchers:Adalberto Luiz Rosa ; Paulo Tambasco de Oliveira


Integrins are a family of membrane receptors that primarily mediate adhesion of cells to extracellular matrix proteins and some members are involved in the process of osteoblast differentiation and bone formation, key events of titanium (Ti) implant osseointegration. It is well known that Ti surfaces with micro and nanotopography favor matrix mineralization but the cellular mechanisms implicated in this process are not entirely understood. In this context, we hypothesized that integrins may have important role in the osteoblast differentiation induced by micro and nanostructured Ti surfaces. To test this hypothesis rat bone marrow mesenchymal stem cells will be cultured on Ti discs with three different surfaces, (1) nanostructured, (2) microstructured and (3) machined (untreated - control) and the following aspects will be evaluated: (1) presence of focal adhesions by vinculin immunolabeling, (2) gene expression of members of the integrin signaling pathway by PCR array, (3) gene expression of bone markers by real-time PCR, (4) protein expression of members of the integrin signaling pathway and bone markers by Western blot and (5) effect of gene silencing using siRNA of integrin signaling pathway members on osteoblast differentiation induced by different Ti surface topographies by real-time PCR and western blot. The outcomes of this study could contribute to clarify the intracellular mechanisms involved in osteoblast responses to Ti and consequently novel strategies using integrins and surface topography could be developed in order to favor the process of osseointegration. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HELENA BACHA LOPES; ALANN THAFFARELL PORTILHO SOUZA; GILEADE PEREIRA FREITAS; CARLOS NELSON ELIAS; ADALBERTO LUIZ ROSA; MARCIO MATEUS BELOTI. Effect of focal adhesion kinase inhibition on osteoblastic cells grown on titanium with different topographies. Journal of Applied Oral Science, v. 28, . (13/05181-3, 14/08443-1, 16/21116-5)
LOPES, HELENA BACHA; FREITAS, GILEADE PEREIRA; CARVALHO FANTACINI, DAIANNE MACIELY; PICANCO-CASTRO, VIRGINIA; COVAS, DIMAS TADEU; ROSA, ADALBERTO LUIZ; BELOTI, MARCIO MATEUS. Titanium with nanotopography induces osteoblast differentiation through regulation of integrin alpha V. Journal of Cellular Biochemistry, v. 120, n. 10, p. 16723-16732, . (13/05181-3, 16/21116-5, 14/08443-1)
LOPES, H. B.; ELIAS, C. N.; OLIVEIRA, P. T.; ROSA, A. L.; BELOTI, M. M.. Effect of titanium surface topography on integrin signaling pathway and osteoblast genotype expression.. MOLECULAR BIOLOGY OF THE CELL, v. 27, p. 1-pg., . (14/08443-1, 13/05181-3)
LOPES, HELENA B.; FREITAS, GILEADE P.; ELIAS, CARLOS N.; TYE, CORALEE; STEIN, JANET L.; STEIN, GARY S.; LIAN, JANE B.; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Participation of integrin beta 3 in osteoblast differentiation induced by titanium with nano or microtopography. Journal of Biomedical Materials Research Part A, v. 107, n. 6, p. 1303-1313, . (13/05181-3, 16/21116-5, 14/08443-1)

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