Salmonella enterica: roles of NAPS in pathogenicity and virulence

• Grant number: 14/13412-8
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2014 - March 31, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Marcelo Brocchi
• Grantee: Marcelo Brocchi
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Recent results from our group indicated that mutants of Salmonella enterica for genes encoding NAPs (nucleoid-associated proteins) are virulence attenuated and able to induce, in different extension, protection in the mouse model of infection. These results suggest an important role of NAPs in bacterial virulence. Based on this, the aim of this project is further study the role of different NAPs in the biology of S. enterica Typhimurium and Enteritidis. New mutants will be constructed and phenotypically evaluated with regard growth in vitro, acid-resistance, bile salts, invasion, persistence and multiplication in macrophages and biofilm formation. Virulence attenuation and immunogenicity will be evaluated in the murine model of infection. Also, high-throughput methodologies will be employed to study gene expression. The results will bring new information on the role of NAPs in S. enterica virulence. In addition, attenuated mutants of S. enterica will be evaluated regarding the therapeutic effect on tumors in the mouse model. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MILANEZ, GUILHERME P.; WERLE, CATIERINE H.; AMORIM, MARIENE R.; RIBEIRO, RAFAEL A.; TIBO, LUIZ H. S.; ROQUE-BARREIRA, MARIA CRISTINA; OLIVEIRA, ALINE F.; BROCCHI, MARCELO. HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection. FRONTIERS IN MICROBIOLOGY, v. 9, AUG 22 2018. (13/04088-0, 12/05382-6, 14/13412-8, 14/05359-0)

RAMPAZZO, P. E.; MARCOS, F. C. C.; CIPRIANO, M. A. P.; MARCHIORI, P. E. R.; FREITAS, S. S.; MACHADO, E. C.; NASCIMENTO, L. C.; BROCCHI, M.; RIBEIRO, R. V.. Rhizobacteria improve sugarcane growth and photosynthesis under well-watered conditions. Annals of Applied Biology, v. 172, n. 3, p. 309-320, MAY 2018. (08/57495-3, 14/13412-8)

MILANEZ, GUILHERME PAIER; NASCIMENTO, LEANDRO COSTA; TIRABASSI, ADRIANE HOLTZ; ZUANAZE, MARCELO; RODRIGUES, DALIA PRAZERES; GUIMARAES PEREIRA, GONCALO AMARANTE; BROCCHI, MARCELO. Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 3, MAY-JUN 2016. (14/13412-8, 12/05382-6)

WERLE, CATIERINE HIRSCH; DAMIANI, IGOR; MILANEZ, GUILHERME PAIER; FARIAS, ALESSANDRO S.; CINTRA GOMES MARCONDES, MARIA CRISTINA; CULLER, HEBERT FABRICIO; SIRCILI, MARCELO PALMA; LEITE, BRUNA; BROCCHI, MARCELO. Antimelanoma effect of Salmonella Typhimurium integration host factor mutant in murine model. FUTURE ONCOLOGY, v. 12, n. 20, p. 2367-2378, OCT 2016. (12/25426-8, 12/05382-6, 14/13412-8)