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The first molecular characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: 14 Culicidae) vitellogenin expression products and a partial cDNA sequence isolation

Grant number: 14/21076-8
Support type:Regular Research Grants - Publications - Scientific article
Duration: November 01, 2014 - April 30, 2015
Field of knowledge:Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
Principal Investigator:Margareth de Lara Capurro-Guimarães
Grantee:Margareth de Lara Capurro-Guimarães
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Anopheles (Nyssorhynchus) aquasalis Curry, 1932, is an anopheline that plays an important role in Plasmodium transmission in the New World. Few studies have explored the molecular reproductive biology of this vector species. In the current study, we isolated and sequenced a partial,886 bp, cDNA fragment of a vitellogenin (Vtg) gene from An. aquasalis. The transcription proÞle of this Vtg gene in adult females was characterized, and it is similar to other members of the Culicidae. We also detected a smaller subunit of An. aquasalis Vtg proteins in ovary tissue and characterized itstranslational proÞle in previtellogenic and vitellogenic females. Our results represent the Þrst molecularcharacterization of An. aquasalis vitellogenin expression products. (AU)