The first molecular characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: 14 Culicidae) vitellogenin expression products and a partial cDNA sequence isolation

• Grant number: 14/21076-8
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: November 01, 2014
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Margareth de Lara Capurro-Guimarães
• Grantee: Margareth de Lara Capurro-Guimarães
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Anopheles (Nyssorhynchus) aquasalis Curry, 1932, is an anopheline that plays an important role in Plasmodium transmission in the New World. Few studies have explored the molecular reproductive biology of this vector species. In the current study, we isolated and sequenced a partial,886 bp, cDNA fragment of a vitellogenin (Vtg) gene from An. aquasalis. The transcription proÞle of this Vtg gene in adult females was characterized, and it is similar to other members of the Culicidae. We also detected a smaller subunit of An. aquasalis Vtg proteins in ovary tissue and characterized itstranslational proÞle in previtellogenic and vitellogenic females. Our results represent the Þrst molecularcharacterization of An. aquasalis vitellogenin expression products. (AU)