Research Grants 14/21076-8 - Expressão gênica, Malária - BV FAPESP
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The first molecular characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: 14 Culicidae) vitellogenin expression products and a partial cDNA sequence isolation

Abstract

Anopheles (Nyssorhynchus) aquasalis Curry, 1932, is an anopheline that plays an important role in Plasmodium transmission in the New World. Few studies have explored the molecular reproductive biology of this vector species. In the current study, we isolated and sequenced a partial,886 bp, cDNA fragment of a vitellogenin (Vtg) gene from An. aquasalis. The transcription proÞle of this Vtg gene in adult females was characterized, and it is similar to other members of the Culicidae. We also detected a smaller subunit of An. aquasalis Vtg proteins in ovary tissue and characterized itstranslational proÞle in previtellogenic and vitellogenic females. Our results represent the Þrst molecularcharacterization of An. aquasalis vitellogenin expression products. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)