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Evaluation of the intestinal microbiota in nonalcoholic fatty liver disease (NAFLD

Grant number: 13/06828-0
Support type:Regular Research Grants
Duration: October 01, 2014 - December 31, 2016
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Claudia Pinto Marques Souza de Oliveira
Grantee:Claudia Pinto Marques Souza de Oliveira
Home Institution: Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Although there is evidence that the intestinal microbiota (MI) is involved in the pathogenesis of obesity, nonalcoholic fatty liver disease (NAFLD) has not been well characterized. The hypotheses put forward to explain the improvement in liver histology, inflammation and insulin sensitivity with the use of probiotics is still very scarce and remains obscure. Following this line of thought it is necessary further investigation of the microbiota, which will help to unravel the numerous questions the pathophysiology and therapy on NAFLD. This study will include 30 patients with nonalcoholic fatty liver disease (NAFLD) (15 patients with steatosis, 15 patients with hepatitis-esteat) enrolled in the Clinic of nonalcoholic fatty liver disease (A2MG700) Discipline of Clinical Gastroenterology, Hospital das Clinicas Faculty of Medicine, University of São Paulo (HC-USP), who have performed liver biopsy, indicated according to the protocol of routine care of this service. The control group will consist of 10 healthy individuals with normal BMI without diabetes or chronic gastrointestinal symptoms. All patients carry Anthropometric and body composition, as well as analysis of clinical, collection of faeces and serum tests. After 7-day food record, stool samples are collected with the help of collector FISHER, and stored at -20 º C for later analysis by pyrosequencing. (AU)