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Red blood cell disorders: pathophysiology and new therapeutic approaches

Grant number: 14/00984-3
Support type:Research Projects - Thematic Grants
Duration: December 01, 2014 - November 30, 2020
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Fernando Ferreira Costa
Grantee:Fernando Ferreira Costa
Home Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Co-Principal Investigators:Carla Fernanda Franco Penteado ; Margareth Castro Ozelo ; Maria de Fatima Sonati ; Mônica Barbosa de Melo ; Nicola Amanda Conran Zorzetto
Assoc. researchers: Aderson da Silva Araujo ; Berengere Koehl ; Bryan Eric Strauss ; Camila Bononi de Almeida ; Carolina Lanaro ; Caroline Le Van Kim ; Chung Man Chin ; Clarisse Lopes de Castro Lobo ; Daniela Maria Ribeiro ; Dulcinéia Martins de Albuquerque ; Erich Vinicius de Paula ; Flávia Costa Leonardo ; Iscia Teresinha Lopes Cendes ; Jean Leandro dos Santos ; Kleber Yotsumoto Fertrin ; Lilian Maria de Castilho ; Magnun Nueldo Nunes dos Santos ; Marcos André Cavalcanti Bezerra ; Marcus Alexandre Finzi Corat ; Marilda de Souza Goncalves ; Mário Angelo Claudino ; Munir Salomao Skaf ; Susan Elisabeth Domingues Costa Jorge ; Tereza Maria Dantas de Medeiros ; Wassim El Nemer
Associated grant(s):16/08342-6 - Multi-user equipment approved in grant 014/00984-3: ECIS (electric cell-substrate impedance sensing), AP.EMU
15/05062-0 - Multi-User Equipment approved in grant 014/00984-3: Imagestream X MarkII, AP.EMU
Associated scholarship(s):19/19648-7 - Red blood cells diseases: physiopathology and new therapeutic aproaches, BP.TT
19/14453-3 - Analysis of the number of f cells in peripheral blood of patients with sickle cell anemia and its correlation with clinical and laboratory parameters, BP.IC
19/13839-5 - Evaluation of the mechanisms involved in static and flow cell interactions of blood outgrowth endothelial cells (BOECs) from patients with negative BCR-ABL1 myeloproliferative neoplasms, BP.IC
+ associated scholarships 18/23484-7 - Evaluation of the efficacy and safety of a strategy based on gene therapy with a viral vector for expression of hemopexin in animal models of sickle cell disease, BP.DR
18/19846-0 - Overexpression of PIPKIIa enzyme gene in normal erythroid cell cultures and patients with hemoglobin H disease, BP.TT
17/23305-2 - Association between hemolysis intensity and clinical evolution in patients with sickle cell disease, BP.PD
18/09576-6 - Analysis of the number of f cells in peripheral blood of patients with sickle cell anemia and its correlation with clinical and laboratory parameters, BP.IC
18/01367-9 - Molecular mechanisms of fetal hemoglobin induction in eritroblasts of patients with bO/bO Thalassemia and in heterozygous individuals with non-deletional hereditary persistence of fetal hemoglobin (Brazilian type), BP.DR
18/04581-1 - Genotyping and mating programmed for genetically modified animal production with sickle cell anemia and persistence of fetal hemoglobin after birth, BP.IC
18/03461-2 - Overexpression of PIPKIIa enzyme gene in normal erythroid cell cultures and patients with hemoglobin h disease, BP.TT
17/21892-8 - Functional analysis of LXN gene in vitro and in vivo and genes global expression evaluation in primary erythroid culture after HbF induction through the compound decitabine, BP.PD
17/14594-0 - TGF-beta action on leukocyte polarization in sickle cell anemia, BP.PD
17/11601-6 - Aspects of angiogenesis in Sickle Cell Anemia: the role of platelets, BP.DD
17/03954-6 - Evaluation of blood pressure and changes in the renin-angiotensin system in an animal model of sickle cell anemia, BP.MS
16/13224-2 - MicroRNAs expression profile and their influence on asthma experimental model in sickle cell transgenic mice, BP.MS
16/22061-0 - Genomic editing with CRISPR/Cas9 for production of hereditary persistance of fetal haemoglobin Brazilian type as sickle cell disease treatment strategy, BP.PD
15/08330-5 - Transcriptomics in placentas of women carrier of sickle cell disease, BP.DR
15/26929-1 - In vivo evaluation of the role of eosinophil in the vaso-occlusion process using sickle cell transgenic mice: involvement of Rho-quinase pathway, BP.MS
16/08729-8 - Investigation of the inflammasome formation in patients with sickle cell anemia, BP.DD
16/08072-9 - Characterization of molecular changes in patients with aceruloplasminemia, BP.MS
15/18369-6 - Evaluation of the role of platelets in sickle cell disease, BP.DR
16/05014-8 - Evaluation of static and flow cell interactions of blood outgrowth endothelial cells mechanisms in patients with Polycythemia Vera, BP.IC
16/04706-3 - Standardization of human hemoglobin purification and crystallization, BP.IC
15/24666-3 - Investigation of new pathophysiological mechanisms of hypercoagulability in sickle cell disease, BP.DR
15/21184-8 - THP-1 culturing cells standardization to evaluate miRNAs and their target genes response against influence of anti-inflammatory stimuli, BP.IC
15/24029-3 - Analysis of susceptibility genes to leg ulcer in patients with sickle cell disease, BP.DD
15/22192-4 - Evaluation of adhesive, procoagulant and inflammatory properties mediated by in vitro interactions of circulating progenitor endothelial cells and endothelial cells differentiated from IPS of patients with sickle cell disease, BP.PD
15/16188-4 - Hybrids of thalidomide + furoxan derivatives : evaluation of anti - inflammatory properties and induction of gamma globin in vitro, BP.MS
15/16517-8 - Inflammatory mediators, interaction with eosinophils and tyrosine kinase inhibitors effects on mast cells of sickle cell anemia patients, BP.PD
15/13970-3 - Functional analysis of LXN gene in vitro and in vivo and genes global expression evaluation in primary erythroid culture after HbF induction through the compound decitabine, BP.PD
15/14255-6 - RNA-seq technique application in endothelial and leukocyte cells transcription profile evaluation in sickle cell retinopathy, BP.PD
15/13710-1 - Structural and functional study of human hemoglobin variants, BP.PD
15/07637-0 - Morphological characteristics of leukocytes and platelets from patients with sickle cell anemia and thalassemia: analysis by image cytometry (Amnis ImageStream), BP.PD
15/04485-4 - Investigation of the renin-angiotensin system in mice with sickle cell anemia, BP.IC
15/04179-0 - Western Blotting technique standardization of protein quantification after PIPKIIa enzyme gene overexpression in human erythroid cell cultures, BP.IC - associated scholarships

Abstract

This new Multi-PI thematic project proposal will be conducted by the Red Blood Cell Research Group, which is formed by researchers from the Hemocentro (Blood Center), the Department of Clinical Pathology, School of Medical Sciences and the Molecular Biology and Genetic Engineering Center (CBMEG) at UNICAMP, in collaboration with several Brazilian and international researchers. The new project represents a major expansion of the previous proposal with the inclusion of new and unprecedented themes, which will undoubtedly result in extremely significant data, from a scientific point of view, and improvements in the therapy of the diseases studied. This project aims to reveal important aspects of red blood cell disease pathophysiology and to discover new forms of treatment, using state of the art equipment and technology, and to broaden the international collaborations formed in recent years. New methods and equipment will be introduced; an intravital microscopy platform for investigation of the microcirculation in vivo, the Amnis ImageStream equipment for the visualization of interactions and cellular alterations, studies using iPS techniques and ultimate generation gene sequencing and functional methods. Taken as a whole, the project proposes studies in five broad areas related to changes in erythrocytes and subsequent diseases; the identification of genes associated with erythropoiesis and molecular factors that regulate the production of HbF, the effects of hemolysis and identification of therapeutic targets, structural and functional changes of the hemoglobin molecule, aspects of the pathophysiology of red blood cell-related diseases and, finally, new drugs and therapeutic targets for the Hemoglobinopathies. The exceptional team of Brazilian and foreign researchers involved in the proposal, associated with the use of methods and equipment described, represents a modern multidisciplinary approach with the use of in vitro and in vivo methods for researching aspects of red cell diseases that have not yet been clarified. We believe that this approach will provide fundamental data for the knowledge of these diseases and the development of new therapies for their treatment. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Sickle cell disease needs more attention 
Research identifies drug capable of preventing complications from hemolysis 
Articles published in other midia outlets (20 total):
More itemsLess items
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Sickle cell disease needs more attention 
Doenças falciformes ainda demandam atenção, asseguram especialistas 
Doenças falciformes ainda demandam atenção 
Apesar de tratamento avançar, doenças falciformes crescem em países pobres 
Apesar de tratamento avançar, doenças falciformes crescem em países pobres 
Doenças falciformes ainda demandam atenção 
Doenças falciformes ainda demandam atenção 
Doenças falciformes ainda demandam atenção 
Doenças falciformes ainda demandam atenção 
Doenças falciformes ainda demandam atenção 
Doenças falciformes ainda demandam atenção e necessidade de novos tratamentos 
Apesar de tratamento avançar, doenças falciformes crescem em países pobres 

Scientific publications (28)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DUARTE, BRUNO K. L.; YAMAGUTI-HAYAKAWA, GABRIELA G.; MEDINA, SAMUEL S.; SIQUEIRA, LUCIA H.; SNETSINGER, BROOKE; COSTA, FERNANDO F.; RAUH, MICHAEL J.; OZELO, MARGARETH C. Longitudinal sequencing of RUNX1 familial platelet disorder: new insights into genetic mechanisms of transformation to myeloid malignancies. British Journal of Haematology, v. 186, n. 5, p. 724-734, SEP 2019. Web of Science Citations: 0.
DE CARVALHO-SIQUEIRA, GABRIELA QUEILA; ANANINA, GALINA; DE SOUZA, BRUNO BATISTA; BORGES, MURILO GUIMARAES; ITO, MIRTA TOMIE; DA SILVA-COSTA, SUELI MATILDE; DOMINGOS, IGOR DE FARIAS; FALCAO, DIEGO ARRUDA; LOPES-CENDES, ISCIA; CAVALCANTI BEZERRA, MARCOS ANDRE; ARAUJO, ADERSON DA SILVA; LUCENA-ARAUJO, ANTONIO ROBERTO; GONCALVES, MARILDA DE SOUZA; OLALLA SAAD, SARA TERESINHA; COSTA, FERNANDO FERREIRA; DE MELO, MONICA BARBOSA. Highlight article: Whole-exome sequencing indicates FLG2 variant associated with leg ulcers in Brazilian sickle cell anemia patients. Experimental Biology and Medicine, v. 244, n. 11, p. 932-939, AUG 2019. Web of Science Citations: 0.
VENDRAME, FELIPE; OLOPS, LETICIA; OLALLA SAAD, SARA TERESINHA; FERREIRA COSTA, FERNANDO; YOTSUMOTO FERTRIN, KLEBER. Hypocholesterolemia and dysregulated production of angiopoietin-like proteins in sickle cell anemia patients. CYTOKINE, v. 120, p. 88-91, AUG 2019. Web of Science Citations: 0.
CRUZ, PEDRO R. S.; ANANINA, GALINA; GIL-DA-SILVA-LOPES, VERA LUCIA; SIMIONI, MILENA; MENAA, FARID; BEZERRA, MARCOS A. C.; DOMINGOS, IGOR F.; ARAUJO, ADERSON S.; PELLEGRINO, RENATA; HAKONARSON, HAKON; COSTA, FERNANDO F.; DE MELO, MONICA BARBOSA. Genetic comparison of sickle cell anaemia cohorts from Brazil and the United States reveals high levels of divergence. SCIENTIFIC REPORTS, v. 9, JUL 26 2019. Web of Science Citations: 0.
BORGES, MARINA DORIGATTI; DE ALBUQUERQUE, DULCINEIA MARTINS; LANARO, CAROLINA; COSTA, FERNANDO FERREIRA; FERTRIN, KLEBER YOTSUMOTO. Clinical relevance of heterozygosis for aceruloplasminemia. AMERICAN JOURNAL OF MEDICAL GENETICS PART B-NEUROPSYCHIATRIC GENETICS, v. 180, n. 4, p. 266-271, JUN 2019. Web of Science Citations: 0.
BAPTISTA, LETICIA C.; FIGUEIRA, CAMILLA O.; SOUZA, BRUNO B.; FERTRIN, KLEBER Y.; ANTOLINI, ARTHUR; COSTA, FERNANDO F.; DE MELO, MONICA B.; COSTA, MARIA LAURA. Highlight Article: Different morphological and gene expression profile in placentas of the same sickle cell anemia patient in pregnancies of opposite outcomes. Experimental Biology and Medicine, v. 244, n. 5, p. 395-403, APR 2019. Web of Science Citations: 0.
LERCHNER, J.; VOLPE, P. O. L.; LANARO, C.; FERTRIN, K. Y.; COSTA, F. F.; ALBUQUERQUE, D. M.; HAENSCHKE, F.; MERTENS, F. A chip calorimetry-based method for the real-time investigation of metabolic activity changes in human erythrocytes caused by cell sickling. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, v. 136, n. 2, p. 771-781, APR 2019. Web of Science Citations: 1.
CONRAN, NICOLA; TORRES, LIDIANE. cGMP modulation therapeutics for sickle cell disease. Experimental Biology and Medicine, v. 244, n. 2, p. 132-146, FEB 2019. Web of Science Citations: 1.
OLATUNYA, OLADELE S.; ALBUQUERQUE, DULCINEIA M.; ADEKILE, ADEKUNLE; COSTA, FERNANDO F. Influence of alpha thalassemia on clinical and laboratory parameters among nigerian children with sickle cell anemia. JOURNAL OF CLINICAL LABORATORY ANALYSIS, v. 33, n. 2 FEB 2019. Web of Science Citations: 0.
TORRES, LIDIANE; CONRAN, NICOLA. Emerging pharmacotherapeutic approaches for the management of sickle cell disease. EXPERT OPINION ON PHARMACOTHERAPY, v. 20, n. 2, p. 173-186, JAN 22 2019. Web of Science Citations: 1.
PEDROSO, GISELE A.; KIMURA, ELZA M.; SANTOS, MAGNUN N. N.; ALBUQUERQUE, DULCINEIA M.; MALIMPENSA, DANAE; JORGE, SUSAN E.; VERISSIMO, MONICA P. A.; COSTA, FERNANDO F.; SONATI, MARIA F. Thalassemia major phenotype caused by HB Zurich-Albisrieden [alpha 2 59(E8) Gly > Arg (HBA2:C.178G > C)] in a Brazilian child. PEDIATRIC BLOOD & CANCER, v. 65, n. 12 DEC 2018. Web of Science Citations: 0.
VENDRAME, FELIPE; OLOPS, LETICIA; SAAD, SARA TERESINHA OLALLA; COSTA, FERNANDO FERREIRA; FERTRIN, KLEBER YOTSUMOTO. Differences in heme and hemopexin content in lipoproteins from patients with sickle cell disease. JOURNAL OF CLINICAL LIPIDOLOGY, v. 12, n. 6, p. 1532-1538, DEC 2018. Web of Science Citations: 2.
SILVA, FABIO HENRIQUE; FERTRIN, KLEBER YOTSUMOTO; ALEXANDRE, EDUARDO COSTA; CALMASINI, FABIANO BERALDI; FRANCO-PENTEADO, CARLA FERNANDA; COSTA, FERNANDO FERREIRA. Impairment of Nitric Oxide Pathway by Intravascular Hemolysis Plays a Major Role in Mice Esophageal Hypercontractility: Reversion by Soluble Guanylyl Cyclase Stimulator. Journal of Pharmacology and Experimental Therapeutics, v. 367, n. 2, p. 194-202, NOV 1 2018. Web of Science Citations: 0.
FERREIRA DE MELO, THAIS REGINA; KUMKHAEK, CHUTIMA; DOS SANTOS FERNANDES, GUILHERME FELIPE; LOPES PIRES, MARIA ELISA; CHELUCCI, RAFAEL CONSOLIN; BARBIERI, KARINA PEREIRA; COELHO, FERNANDA; DE OLIVEIRA CAPOTE, TICIANA SIDORENKO; LANARO, CAROLINA; CARLOS, IRACILDA ZEPPONE; MARCONDES, SISI; CHEGAEV, KONSTANTIN; GUGLIELMO, STEFANO; FRUTTERO, ROBERTA; CHUNG, MAN CHIN; COSTA, FERNANDO FERREIRA; RODGERS, GRIFFIN P.; DOS SANTOS, JEAN LEANDRO. Discovery of phenylsulfonylfuroxan derivatives as gamma globin inducers by histone acetylation. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, v. 154, p. 341-353, JUN 25 2018. Web of Science Citations: 0.
JORGE, S. E.; BRINGAS, M.; PETRUK, A. A.; ARRAR, M.; MARTI, M. A.; SKAF, M. S.; COSTA, F. F.; CAPECE, L.; SONATI, M. F.; ESTRIN, D. Understanding the molecular basis of the high oxygen affinity variant human hemoglobin Coimbra. Archives of Biochemistry and Biophysics, v. 637, p. 73-78, JAN 1 2018. Web of Science Citations: 2.
MOTA, NATALIA O.; KIMURA, ELZA M.; FERREIRA, ROBERTA D.; PEDROSO, GISELE A.; ALBUQUERQUE, DULCINEIA M.; RIBEIRO, DANIELA M.; SANTOS, MAGNUN N. N.; BITTAR, CRISTINA M.; COSTA, FERNANDO F.; SONATI, MARIA DE FATIMA. Rare alpha(0)-thalassemia deletions detected by MLPA in five unrelated Brazilian patients. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 4, p. 768-773, OCT-DEC 2017. Web of Science Citations: 1.
DE SOUZA, GLEICE REGINA; HOUNKPE, BIDOSSESSI WILFRIED; LUZ FIUSA, MAIARA MARX; COLELLA, MARINA PEREIRA; ANNICHINO-BIZZACCHI, JOYCE M.; TRAINA, FABIOLA; COSTA, FERNANDO FERREIRA; DE PAULA, ERICH VINICIUS. Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. PLoS One, v. 12, n. 4 APR 24 2017. Web of Science Citations: 1.
NATÁLIA O. MOTA; ELZA M. KIMURA; ROBERTA D. FERREIRA; GISELE A. PEDROSO; DULCINÉIA M. ALBUQUERQUE; DANIELA M. RIBEIRO; MAGNUN N. N. SANTOS; CRISTINA M. BITTAR; FERNANDO F. COSTA; MARIA DE FATIMA SONATI. Rare α0-thalassemia deletions detected by MLPA in five unrelated Brazilian patients. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 4, p. -, Dez. 2017.
PEDROSO, GISELE A.; KIMURA, ELZA M.; SANTOS, MAGNUN N. N.; ALBUQUERQUE, DULCINEIA M.; FERRUZZI, JUCILANE L. H.; JORGE, SUSAN E.; COSTA, FERNANDO F.; SAAD, SARA T. O.; SONATI, MARIA F. Coinheritance of Hb Bristol-Alesha [beta 67(E11)Val -> Met; HBB: c.202G > A] and the alpha 212 Patchwork Allele in a Brazilian Child with Severe Congenital Hemolytic Anemia. HEMOGLOBIN, v. 41, n. 3, p. 203-208, 2017. Web of Science Citations: 1.
SILVA, FABIO H.; CLAUDINO, MARIO A.; CALMASINI, FABIANO B.; ALEXANDRE, EDUARDO C.; FRANCO-PENTEADO, CARLA; BURNETT, ARTHUR L.; ANTUNES, EDSON; COSTA, FERNANDO F. Sympathetic Hyperactivity, Increased Tyrosine Hydroxylase and Exaggerated Corpus Cavernosum Relaxations Associated with Oxidative Stress Plays a Major Role in the Penis Dysfunction in Townes Sickle Cell Mouse. PLoS One, v. 11, n. 12 DEC 9 2016. Web of Science Citations: 3.
SILVA, FABIO H.; KARAKUS, SERKAN; MUSICKI, BILJANA; MATSUI, HOTAKA; BIVALACQUA, TRINITY J.; DOS SANTOS, JEAN L.; COSTA, FERNANDO F.; BURNETT, ARTHUR L. Beneficial Effect of the Nitric Oxide Donor Compound 3-(1,3-Dioxoisoindolin-2-yl)Benzyl Nitrate on Dysregulated Phosphodiesterase 5, NADPH Oxidase, and Nitrosative Stress in the Sickle Cell Mouse Penis: Implication for Priapism Treatment. Journal of Pharmacology and Experimental Therapeutics, v. 359, n. 2, p. 230-237, NOV 1 2016. Web of Science Citations: 5.
BAPTISTA, LETICIA C.; COSTA, MARIA LAURA; FERREIRA, REGIANE; ALBUQUERQUE, DULCINEIA M.; LANARO, CAROLINA; FERTRIN, KLEBER Y.; SURITA, FERNANDA G.; PARPINELLI, MARY A.; COSTA, FERNANDO F.; DE MELO, MONICA BARBOSA. Abnormal expression of inflammatory genes in placentas of women with sickle cell anemia and sickle hemoglobin C disease. ANNALS OF HEMATOLOGY, v. 95, n. 11, p. 1859-1867, NOV 2016. Web of Science Citations: 3.
MARIA SOLER, ANA; SCHELOTTO, MAGDALENA; DE OLIVEIRA MOTA, NATALIA; DORTA FERREIRA, ROBERTA; DE FATIMA SONATI, MARIA; ABAYUBA DA LUZ, JULIO. The -()(5.2) Deletion Detected in a Uruguayan Family: First Case Report in the Americas. HEMOGLOBIN, v. 40, n. 4, p. 289-292, AUG 2016. Web of Science Citations: 0.
GASPARDI, ANE C.; SIPPERT, EMILIA A.; DE MACEDO, MAYRA DORIGAN; PELLEGRINO, JR., JORDAO; COSTA, FERNANDO F.; CASTILHO, LILIAN. Clinically relevant RHD-CE genotypes in patients with sickle cell disease and in African Brazilian donors. BLOOD TRANSFUSION, v. 14, n. 5, p. 449-454, 2016. Web of Science Citations: 5.
LIMA, KELI; RIBEIRO, DANIELA MARIA; CAMPOS, PAULA DE MELO; COSTA, FERNANDO FERREIRA; TRAINA, FABIOLA; OLALLA SAAD, SARA TERESINHA; SONATI, MARIA DE FATIMA; MACHADO-NETO, JOAO AGOSTINHO. Differential profile of PIP4K2A expression in hematological malignancies. BLOOD CELLS MOLECULES AND DISEASES, v. 55, n. 3, p. 228-235, OCT 2015. Web of Science Citations: 2.
LEONARDO, DANIELA P.; ALBUQUERQUE, DULCINELA M.; LANARO, CAROLINA; BAPTISTA, LETICIA C.; CECATTI, JOSE G.; SURITA, FERNANDA G.; PARPINELLI, MARY A.; COSTA, FERNANDO F.; FRANCO-PENTEADO, CARLA F.; FERTRIN, KLEBER Y.; COSTA, MARIA LAURA. Association of Nitric Oxide Synthase and Matrix Metalloprotease Single Nucleotide Polymorphisms with Preeclampsia and Its Complications. PLoS One, v. 10, n. 8 AUG 28 2015. Web of Science Citations: 13.
MAGNUN NUELDO NUNES DOS SANTOS. Haptoglobin: an emerging candidate for phenotypic modulation of sickle cell anemia?. Revista Brasileira de Hematologia e Hemoterapia, v. 37, n. 6, p. 361-363, Dez. 2015.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
COSTA, Fernando Ferreira. . 2016. Master's Dissertation - Universidade Estadual de Campinas, Faculdade de Ciências Médicas.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
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