| Grant number: | 14/11544-4 |
| Support Opportunities: | Regular Research Grants |
| Start date: | December 01, 2014 |
| End date: | December 31, 2017 |
| Field of knowledge: | Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms |
| Principal Investigator: | Jorge Maurício Costa Mondego |
| Grantee: | Jorge Maurício Costa Mondego |
| Host Institution: | Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil |
| City of the host institution: | Campinas |
| Associated researchers: | Carlos Augusto Colombo ; Marcelo Brocchi ; Marcelo Falsarella Carazzolle ; Márcio José da Silva |
Abstract
Pseudomonas putida is a bacterial species with ability to colonize a wide range of environments. P. putida is known mainly as plant growth promoting bacteria. The production of auxins and siderophores by P. putida lead to the improvement of vegetal development, confer yield increment and give better protection against pathogens. During the annotation of C. arabica ESTs databank, we detected a considerable amount of Pseudomonas sequences, what suggest that Pseudomonads inhabit C. arabica plants. Through the isolation of bacteria from leaves (phylosphere), rizosphere and from the internal environment (endophytics) of C. arabica roots, five P. putida isolates were detected. The production of auxins and siderophores and resistance against caffeine and water stress were tested for the five isolates. Interestingly, a correlation between physiological data and environment of each isolate was detected. These project aims to verify the genomic differences among the isolates, correlating them to preliminary physiological data, which will be repeated and extended during the project. For that, three isolates, each one isolated from a different niche, will have its genome sequenced and compared. Such approach, that have been named as 'pan-genomics', aims to understand why organisms from the same species, sharing the same host, exhibit distinct ecological and physiological behaviors. In addition, these isolates will be inoculated in C. arabica seeds and data from plant growth will be recorded and correlated with data from bacterial physiology and genomics. This knowledge will allow a better exploitation of the potential of such Pseudomonas as coffee growth promoters. (AU)
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