Effect of bone demineralization on tissue repair processes: microscopic, structural and osteoblastic cells activity study in rats calvariae

• Grant number: 14/15136-8
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: August 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Maria Lúcia Rubo de Rezende
• Grantee: Maria Lúcia Rubo de Rezende
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• City of the host institution: Bauru
• Associated researchers: Carla Andreotti Damante ; Carlos Ferreira dos Santos ; Carlos Roberto Grandini ; Marco Antonio Hungaro Duarte ; Paulo Noronha Lisboa Filho ; Rodrigo Cardoso de Oliveira

Abstract

Recent data indicate that the demineralization of vital bone promotes the consolidation of bone grafts and the proliferation and differentiation of pre - osteoblasts in culture, but the biological mechanisms involved in this process remain unclear. In rat calvaria, bone demineralization effects will be investigated for 1) the repair of onlay bone grafts whose contact surfaces shall be demineralized likewise; 2) the behavior of human osteoblasts cultured on demineralized bone surfaces in the same way as a) the cell morphology and surface area coverage by cells under scanning electron microscopy (SEM) ; b) the cellular synthetic activity by polymerase chain reaction in real-time (RT - PCR ) for expression of metalloproteinases (MMP - 2 , MMP -9), osteopontin (OPN), bone sialoprotein (BSP) the progressive ankylosis gene (ANKH), alcaline fosfatase, collagen tipe I and Runx-2; c) the cell ability to deposit calcium by colorimetric method. The demineralized surfaces will be characterized: on the composition through energy dispersive spectroscopy (EDS); for expression of bone morphogenetic proteins (BMPs) by confocal microscopy and for surface roughness by profilometry. Quantitative data collected from conventional optical microscopy, ¼CT, SEM, RT-PCR and colorimetry will be compared by ANOVA and Tukey's post-test and data analysis related to the bone surface will be compared by Student's t test, both with significance level of 95%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROJAS-PAULUS, JEFREY E.; MANFREDI, GUSTAVO G. P.; SALMERON, SAMIRA; CONSOLARO, ALBERTO; SANT'ANA, ADRIANA C. P.; ZANGRANDO, MARIANA S. R.; DAMANTE, CARLA A.; GREGHI, SEBASTIAO L. A.; REZENDE, MARIA L. R.. Citric acid, but not tetracycline, improves the microscopic pattern of healing of particulate autogenous bone grafts in critical-size defects. Journal of Periodontology, v. 92, n. 5, p. 678-688, MAY 2021. (14/15136-8)

DO PRADO MANFREDI, GUSTAVO GONCALVES; TOKUHARA, CINTIA KAZUKO; SALMERON, SAMIRA; SPADA DE CARVALHO, ERIKA BEATRIZ; LIBOA-FILHO, PAULO NORONHA; DAMANTE, CARLA ANDREOTTI; PASSANEZI SANT'ANA, ADRIANA CAMPOS; RAGGHIANTI ZANGRANDO, MARIANA SCHUTZER; AGUIAR GREGHI, SEBASTIAO LUIS; RUBO DE REZENDE, MARIA LUCIA. Bone demineralization promotes superior spread of preosteoblast in culture. MICROSCOPY RESEARCH AND TECHNIQUE, v. 82, n. 7, p. 1004-1011, JUL 2019. (14/15136-8)

CARVALHO, ERIKA B. S.; VERONESI, GIOVANA F.; MANFREDI, GUSTAVO G. P.; DAMANTE, CARLA A.; SANT'ANA, ADRIANA C. P.; GREGHI, SEBASTIAO L. A.; ZANGRANDO, MARIANA S. R.; CONSOLARO, ALBERTO; REZENDE, MARIA L. R.. Bone demineralization improves onlay graft consolidation: A histological study in rat calvaria. Journal of Periodontology, v. 92, n. 6, p. 1-10, JUN 2021. (14/15136-8)