Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury

• Grant number: 14/12381-1
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2015 - January 31, 2017
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Raquel Agnelli Mesquita Ferrari
• Grantee: Raquel Agnelli Mesquita Ferrari
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated researchers: Heloisa Sobreiro Selistre de Araújo ; Kristianne Porta Santos Fernandes

Abstract

Muscle injuries represent a major clinical conditions found in the rehabilitation center. The low level laser (LLL) is a resource that has shown positive results in the literature with regard to muscle repair after the occurrence of different types of lesion, however, little is known about its effects when applied prior to an injury or when there is an association of the prior and pos lesion treatment. The objective of this proposal is to evaluate the effects of LLL therapy (LLLT) on the expression and synthesis of inflammatory cytokines (IL-6 and TNF ±) and proteins involved in muscle repair (myostatin and calcineurin), on the oxidative stress and on the expression of isoforms of Myosin Heavy Chain (MyHC), during the repair process of skeletal muscle of rats. Wistar rats will be divided into four groups: 1) control; 2) cryoinjured without treatment; 3) cryoinjured treated prior with LLLT and 4) cryoinjured treated with LLLT prior e post injury. Analyses will be performed at 1 and 3 days for oxidative stress, 3, 7 and 14 days for assessment of inflammatory cytokines and 3, 7 and 14 days assessment of myostatin, calcineurin MyHC. Cryoinjury will be performed on the belly of the tibialis anterior (TA) muscle. LLLT will be performed daily using the MMOptics GaAlAs laser (wavelength of 780 nm) using an energy density of 10 J/cm², an output power of 40 mW, a beam spot of 0.4 cm² and time of 10 seconds. For the analysis of gene expression will be extracted total RNA of the TA muscle and will obtain cDNA for performing Real-time PCR using specific primers for each gene described. Proteins analysis will be performed using enzyme-linked immunosorbent assays (ELISA) and Western blotting (MyHC), the stress oxidative will be performed by lipid peroxidation (LPO) and the damage to proteins, and the analysis of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Data will be analyzed statistically. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDREO, LUCAS; MESQUITA-FERRARI, RAQUEL A.; RIBEIRO, BEATRIZ G.; BENITTE, ADRIANA; NOGUEIRA, TATIANE DE FATIMA; FRANCA, CRISTIANE M.; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA K.; FERNANDES, KRISTIANNE P. S.; CORREA, FERNANDA I.; et al. Effects of myogenic precursor cells (C2C12) transplantation and low-level laser therapy on muscle repair. Lasers in Surgery and Medicine, v. 50, n. 7, p. 781-791, SEP 2018. (14/12381-1)

ANDREO, LUCAS; SOLDERA, CARLA A.; RIBEIRO, BEATRIZ GUIMARAES; VICENTE DE MATOS, PATRICK RAOUL; SOUSA, PAOLA BINDES; DE ALCANTARA ARAUJO AMORIM, WESLLEY WALLACE; RATTO TEMPESTINI HORLIANA, ANNA CAROLINA; BUSSADORI, SANDRA KALIL; SANTOS FERNANDES, KRISTIANNE PORTA; MESQUITA-FERRARI, RAQUEL AGNELLI. Effects of Photobiomodulation on Functionality in Wistar Rats with Sciatic Nerve Injury. Photochemistry and Photobiology, v. 95, n. 3, p. 879-885, MAY 2019. (14/12381-1, 13/07502-1)

ANDREO, L.; SOLDERA, C. B.; RIBEIRO, B. G.; DE MATOS, P. R. V.; BUSSADORI, S. K.; FERNANDES, K. P. S.; MESQUITA-FERRARI, R. A.. Effects of photobiomodulation on experimental models of peripheral nerve injury. Lasers in Medical Science, v. 32, n. 9, p. 2155-2165, DEC 2017. (14/12381-1, 13/07502-1)

DO VALE, KATIA LLANOS; MARIA, DURVANEI AUGUSTO; PICOLI, LARA CRISTINA; DEANA, ALESSANDRO MELO; MASCARO, MARCELO BETTI; MESQUITA FERRARI, RAQUEL AGNELLI; BUSSADORI, SANDRA KALIL; SANTOS FERNANDES, KRISTIANNE PORTA. The Effects of Photobiomodulation Delivered by Light-Emitting Diode on Stem Cells from Human Exfoliated Deciduous Teeth: A Study on the Relevance to Pluripotent Stem Cell Viability and Proliferation. Photomedicine and Laser Surgery, v. 35, n. 12, p. 659-665, DEC 2017. (13/07502-1, 14/12381-1)

RIBEIRO, BEATRIZ GUIMARAES; ALVES, AGNELO NEVES; DIAS DOS SANTOS, LUCAS ANDREO; SANTOS FERNANDES, KRISTIANNE PORTA; CANTERO, TATIANE MATARAZZO; GOMES, MARIANA TEIXEIRA; FRANCA, CRISTIANE MIRANDA; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. The effect of low-level laser therapy (LLLT) applied prior to muscle injury. Lasers in Surgery and Medicine, v. 47, n. 7, p. 571-578, SEP 2015. (14/12381-1, 12/11461-6, 12/25198-5, 13/21540-3)

DA SILVA JUNIOR, EVALDO MOREIRA; MESQUITA-FERRARI, RAQUEL AGNELLI; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; RAMOS MAGALHAES, ERIC MORENO; BUSSADORI, SANDRA KALIL; DE BRITO, ADRIANA; MESSIAS, FELIPE DE MOURA; COSTA SOUZA, NADHIA HELENA; ALVES, AGNELO NEVES; SANTOS FERNANDES, KRISTIANNE PORTA. The Effects of Photobiomodulation on Inflammatory Infiltrate During Muscle Repair in Advanced-Age Rats. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v. 75, n. 3, p. 437-441, MAR 2020. (13/07502-1, 14/12381-1)

RODRIGUES, DANIELLE DE LIMA; ALVES, AGNELO NEVES; GUIMARAES, BEATRIZ RIBEIRO; DE ALCANTARA ARAUJO AMORIM, WESLLEY WALLACE; BUSSADORI, SANDRA KALIL; SANTOS FERNANDES, KRISTIANNE PORTA; MESQUITA-FERRARI, RAQUEL AGNELLI. Effect of prior application with and without post-injury treatment with low-level laser on the modulation of key proteins in the muscle repair process. Lasers in Medical Science, v. 33, n. 6, p. 1207-1213, AUG 2018. (17/06461-0, 14/12381-1, 13/21540-3)

LINS TERENA, STELLA MARIS; SANTOS FERNANDES, KRISTIANNE PORTA; BUSSADORI, SANDRA KALIL; BRUGNERA JUNIOR, ALDO; TEIXEIRA DA SILVA, DANIELA DE FATIMA; RAMOS MAGALHAES, ERIC MORENO; MESQUITA FERRARI, RAQUEL AGNELLI. Infrared Laser Improves Collagen Organization in Muscle and Tendon Tissue During the Process of Compensatory Overload. Photomedicine and Laser Surgery, v. 36, n. 3, p. 130-136, MAR 2018. (14/12381-1)

VIANA DE MELO, CLAUDIA APARECIDA; ALVES, AGNELO NEVES; LINS TERENA, STELLA MARIS; SANTOS FERNANDES, KRISTIANNE PORTA; NUNES, FABIO DAUMAS; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA KALIL; DEANA, ALESSANDRO MELO; MESQUITA-FERRARI, RAQUEL AGNELLI. Light-emitting diode therapy increases collagen deposition during the repair process of skeletal muscle. Lasers in Medical Science, v. 31, n. 3, p. 531-538, APR 2016. (14/12381-1, 13/21540-3)

RIBEIRO, BEATRIZ GUIMARAES; ALVES, AGNELO NEVES; DIAS DOS SANTOS, LUCAS ANDREO; CANTERO, TATIANE MATARAZZO; SANTOS FERNANDES, KRISTIANNE PORTA; DIAS, DANIELLE DA SILVA; BERNARDES, NATHALIA; DE ANGELIS, KATIA; MESQUITA-FERRARI, RAQUEL AGNELLI. Red and Infrared Low-Level Laser Therapy Prior to Injury with or without Administration after Injury Modulate Oxidative Stress during the Muscle Repair Process. PLoS One, v. 11, n. 4, APR 15 2016. (14/12381-1, 13/21540-3)

ALVES, AGNELO NEVES; RIBEIRO, BEATRIZ GUIMARES; SANTOS FERNANDES, KRISTIANNE PORTA; COSTA SOUZA, NADHIA HELENA; ROCHA, LILIA ALVES; NUNES, FABIO DAUMAS; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Comparative effects of low-level laser therapy pre- and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair. Lasers in Medical Science, v. 31, n. 4, p. 679-685, MAY 2016. (14/12381-1, 13/21540-3, 12/11461-6, 11/04452-8, 11/17638-2)

DA SILVA JUNIOR, EVALDO MOREIRA; MESQUITA-FERRARI, RAQUEL AGNELLI; FRANCA, CRISTIANE MIRANDA; ANDREO, LUCAS; BUSSADORI, SANDRA KALIL; SANTOS FERNANDES, KRISTIANNE PORTA. Modulating effect of low intensity pulsed ultrasound on the phenotype of inflammatory cells. BIOMEDICINE & PHARMACOTHERAPY, v. 96, p. 1147-1153, DEC 2017. (14/12381-1, 13/07502-1)

DE BRITO, ADRIANA; ALVES, AGNELO NEVES; RIBEIRO, BEATRIZ GUIMARAES; EMILIO BARBOSA, DANIEL VICTOR D.; RAMOS MAGALHAES, ERICK MORENO; SANTOS FERNANDES, KRISTIANNE PORTA; BUSSADORI, SANDRA KALIL; GOULARDINS, JULIANA BARBOSA; MESQUITA-FERRARI, RAQUEL AGNELLI. Effect of photobiomodulation on connective tissue remodeling and regeneration of skeletal muscle in elderly rats. Lasers in Medical Science, v. 33, n. 3, p. 513-521, APR 2018. (14/12381-1)

DOS SANTOS, TAINA CAROLINE; SOUSA, KALINE DE BRITO; ANDREO, LUCAS; MARTINELLI, ANDREIA; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; BUSSADORI, SANDRA KALIL; SANTOS FERNANDES, KRISTIANNE PORTA; MESQUITA-FERRARI, RAQUEL AGNELLI. Effect of Photobiomodulation on C2C12 Myoblasts Cultivated in M1 Macrophage-conditioned Media. Photochemistry and Photobiology, v. 96, n. 4, APR 2020. (14/12381-1)

ANDREO, LUCAS; RIBEIRO, BEATRIZ G.; ALVES, AGNELO N.; MARTINELLI, ANDREIA S. A.; SOLDERA, CARLA B.; HORLIANA, ANNA CAROLINA R. T.; BUSSADORI, SANDRA KALIL; FERNANDES, KRISTIANNE P. S.; MESQUITA-FERRARI, RAQUEL A.. Effects of Photobiomodulation with Low-level Laser Therapy on Muscle Repair Following a Peripheral Nerve Injury in Wistar Rats. Photochemistry and Photobiology, APR 2020. (14/12381-1, 13/07502-1)