Multiuser equipment approved in grant # 2014/14457-5: PILOT shelf freeze-dryer

• Grant number: 14/20224-3
• Support Opportunities: Multi-user Equipment Program
• Start date: February 01, 2015
• End date: January 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Eneida de Paula
• Grantee: Eneida de Paula
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Processamento de tecidos - Desidratação (liofilização)
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The pilot freeze dryer is essential to the development of lyophilized parenteral formulations that are reproducible, traceable, scalable, without contaminants and stable. Currently there is no pilot freeze dryer available for drug development in Unicamp. The main focus of our research group for years is the parenteral drug formulation, aimed not only at academic research but also the production of patents and collaboration with the industry, in order to convert the knowledge generated in commercial medicines. By the time we had no access to equipment and methodology for characterization of lyophilized formulations, which is more stabl for parenteral drug dosage form. To meet this demand Dra. Laura de Oliveira Nascimento, with experience in lyophilization of vaccines, will perform the thermal characterization of the products developed and define the lyophilization cycles to be used. Without the pilot lyophilizer, it is not possible to control the rates of heating and cooling ramps of pressure or analytically determine the end of primary drying, which often leads to the collapse of the product. Besides the equipment serving the biomembranes laboratory, it will be employed immediately in: - The new Pharmaceutical Technology laboratory of Dr. Laura de Oliveira Nascimento, newly hired in our department, focused on development of parenteral formulations of antimicrobials and vaccines. The laboratory has an ongoing FAPESP project and a submitted CNPq project that would benefit from the equipment. - The biotechnology laboratory of Dr. Marcelo Lancelotti, focused on development of parenteral vaccines to prevent bacterial meningitis. The laboratory also has a FAPESP regular project and a submitted CNPq project that would benefit from the equipment. - The courses graduate of biochemistry and bioactive products, because with this equipment we can offer a practical discipline of theoretical development of parenteral pharmaceutical formulations. No similar matter in any course of Unicamp. - Several groups of developing drug formulations in Unicamp, including the groups that reach the clinical stage and have no way to define process parameters to produce medicines that meet quality requirements. The latter case is even more relevant in the university, because the product development by empirical means generally not scalable in the preclinical phase may invalidate these results for further developments. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COUTO, VERONICA MUNIZ; DE OLIVEIRA-NASCIMENTO, LAURA; CABECA, LUIZ FERNANDO; GERALDES, DANILO COSTA; RIBEIRO COSTA, JULIANA SOUZA; RISKE, KARIN A.; FRANZ-MONTAN, MICHELLE; YOKAYCHIYA, FABIANO; DIAS FRANCO, MARGARETH K. K.; DE PAULA, ENEIDA. Capsaicin-Cyclodextrin Complex Enhances Mepivacaine Targeting and Improves Local Anesthesia in Inflamed Tissues. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 16, AUG 2020. (14/20224-3, 15/11804-9, 14/14457-5)