Oxidative stress in obese dogs: association with activation of inflammatory response and renal disease

• Grant number: 14/20662-0
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2015 - February 28, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Paulo César Ciarlini
• Grantee: Paulo César Ciarlini
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Valéria Marçal Felix de Lima

Abstract

As in humans, obesity is the most common nutritional disease, a modern epidemic in several areas affects more than 30% of the canine population. In humans and laboratory animals, but not in dogs, is well established relationship between obesity, oxidative stress and renal disease. Renal involvement has been reported in obese dogs, but the association between the accumulation of adipose tissue, activation of the inflammatory response, oxidative stress and renal disease was not as well established as in humans. Recently our research group demonstrated that oxidative stress occurs in dogs with chronic renal disease and a pilot study we observed the activation of neutrophil oxidative metabolism in obese dogs. In this sense a multidisciplinary approach (diagnostic imaging, clinical and laboratory) on canine obesity will be performed in order to obtain results that contribute to the better understanding of the relationship between the inflammatory response, oxidative stress and renal function in obese dogs. For such an analytical and cross-sectional observational clinical study, dogs with good condition (control), with over weight and obese will be held. Obese dogs with kidney disease will undergo a staging as IRIS (2013) criteria. The degree of association between changes in various markers of obesity, inflammatory response, oxidative stress and renal injury be quantified. For this, all dogs will be selected measured changes in markers of obesity (body score, body mass index, plasma adiponectin and leptin), renal injury (UPC, microalbuminuria, plasma cystatin C, renal resistance index and pulsatility), markers inflammatory response (C-reactive protein, TNF., IFN-gamma, IL-2, IL-4 and IL-10, neutrophil production of superoxide and hydrogen peroxide) and oxidative stress markers (albumin, bilirubin, uric acid, GGT , total antioxidant capacity, total concentration of oxidant, plasma MDA). The results will be statistically processed in order to test the following hypotheses: (1) Oxidative stress varies with the degree of obesity of dogs; (2) The concentrations of proinflammatory cytokines vary with the degree of obesity in dogs; (3) In obese dogs increased neutrophil oxidative metabolism occurs; (4) There is an association between plasma concentrations of proinflammatory cytokines and increased neutrophil oxidative metabolism in obese dogs; (5) Increased oxidative metabolism of neutrophils is associated with oxidative stress in obese dogs and (6) Increased oxidative stress is associated with kidney damage and impaired renal blood flow in obese dogs. This proposal is linked to two research projects dissertations and two doctoral projects with the program of Animal Science FMVA-UNESP. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOSCO, A. M.; ALMEIDA, B. F. M.; VALADARES, T. C.; BAPTISTIOLLI, L.; HOFFMANN, D. J.; PEREIRA, A. A. F.; LIMA, V. M. F.; CIARLINI, P. C.. Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v. 202, p. 18-24, AUG 2018. (15/06467-3, 14/20662-0)