Secondary metabolites from aquatic microrganisms and their impact on seafood and freshwater fish aquaculture

• Grant number: 14/50420-9
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: February 28, 2017
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Agreement: Innovation Fund Denmark
• Principal Investigator: Ernani Pinto Junior
• Grantee: Ernani Pinto Junior
• Principal researcher abroad: Kristian Fog Nielsen
• Institution abroad: Technical University of Denmark (DTU), Denmark
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Occurrence of microalgae and cyanobacteria blooms in both freshwater and marine environments frequently occur, often with the release of toxic metabolites (e.g., microcystins, saxitoxins, okadaic acid and its congeners, pectenotoxins, and ichthyotoxins). Other classes of bioactive non-toxic natural products, however, have also been observed for these species but their biochemical and physiological functions still remain unclear. It has been suggested that some of these bioactive metabolites play an important role as allelochemicals and quorum sensing compounds. However, the literature concerning its effects in mammals is rare. This bilateral cooperation intends to use nove I analytical chemistry methods as a tool to investigate metabolites produced by microalgae and cyanobateria and their possible mechanism of action and accumulation in aquatic organisms. The knowledge gained from these experiments will also allow us to discover possible risk for seafood and fish consumers. Experiments will be carried to identify their chemical structures, mechanism of action and risk assessment. Both partners have significant expertise as researchers in this field as well as are coordinators and principal investigators of large-scale projects and interdisciplinary research consortia. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BINZER, SOFIE BJORNHOLT; SVENSSEN, DANIEL KILLERUP; DAUGBJERG, NIELS; ALVES-DE-SOUZA, CATHARINE; PINTO, ERNANI; HANSEN, PER JUEL; LARSEN, THOMAS OSTENFELD; VARGA, ELISABETH. A-, B- and C-type prymnesins are Glade specific compounds and chemotaxonomic markers in Prymnesium parvum. HARMFUL ALGAE, v. 81, p. 10-17, JAN 2019. (14/50420-9)

MIRANDA, MARCELA; MARINHO, MARCELO MANZI; NOYMA, NATALIA; HUSZAR, VERA L. M.; VAN OOSTERHOUT, FRANK; LURLING, MIQUEL; OMETTO, JEAN P.; PACHECO, FELIPE S.. Phosphorus balance in a tropical shallow urban pond in Southeast Brazil: implications for eutrophication management. INLAND WATERS, JUL 2021. (14/50420-9, 14/06556-3)

MIRANDA, MARCELA; NOYMA, NATALIA; PACHECO, FELIPE S.; DE MAGALHAES, LEONARDO; PINTO, ERNANI; SANTOS, SUZAN; SOARES, MARIA FERNANDA A.; HUSZAR, VERA L.; LURLING, MIGUEL; MARINHO, MARCELO M.. The efficiency of combined coagulant and ballast to remove harmful cyanobacterial blooms in a tropical shallow system. HARMFUL ALGAE, v. 65, p. 27-39, MAY 2017. (14/06556-3, 14/50420-9)

SANZ, MIRIAM; DOERR, FELIPE AUGUSTO; PINTO, ERNANI. First report of spumigin production by the toxic Sphaerospermopsis torques-reginae cyanobacterium. Toxicon, v. 108, p. 15-18, DEC 15 2015. (14/50420-9, 12/25272-0, 11/51950-3)

HECK, KARINA; ALVARENGA, DANILLO O.; SHISHIDO, TANIA K.; VARANI, ALESSANDRO M.; DORR, FELIPE A.; PINTO, ERNANI; ROUHIAINEN, LEO; JOKELA, JOUNI; SIVONEN, KAARINA; FIORE, MARLI F.. Biosynthesis of microcystin hepatotoxins in the cyanobacterial genus Fischerella. Toxicon, v. 141, p. 43-50, JAN 2018. (14/50420-9, 11/08092-6, 13/50425-8)

FERNANDES, KELLY; GOMES, ANDREIA; CALADO, LEONARDO; YASUI, GEORGE; ASSIS, DIEGO; HENRY, THEODORE; FONSECA, ANA; PINTO, ERNANI. Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae. TOXINS, v. 11, n. 4, APR 2019. (14/50420-9)

PAIVA, FERNANDA C. R.; FERREIRA, GLAUCIO MONTEIRO; TROSSINI, GUSTAVO H. G.; PINTO, ERNANI. Identification, In Vitro Testing and Molecular Docking Studies of Microginins' Mechanism of Angiotensin-Converting Enzyme Inhibition. Molecules, v. 22, n. 12, DEC 2017. (14/50420-9, 13/08757-3)

FERNANDES, KELLY AFONSINA; FERRAZ, HUMBERTO GOMES; VEREAU, FANNY; PINTO, ERNANI. Availability of Guanitoxin in Water Samples Containing Sphaerospermopsis torques-reginae Cells Submitted to Dissolution Tests. PHARMACEUTICALS, v. 13, n. 11, NOV 2020. (14/50420-9)

FERREIRA, GLAUCIO MONTEIRO; KRONENBERGER, THALES; DE ALMEIDA, ERYKA COSTA; SAMPAIO, JOSEANE; TERRA, CLELIA FERREIRA; PINTO, ERNANI; GOULART TROSSINI, GUSTAVO HENRIQUE. Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins. Molecules, v. 24, n. 23, DEC 2019. (14/50420-9)

VILAS BOAS, LAYNE AMARAL; XAVIER SENRA, MARCUS VINICIUS; FERNANDES, KELLY; DA ANUNCIACAO GOMES, ANDREIA MARIA; PEDROSO DIAS, ROBERTO JUNIO; PINTO, ERNANI; FONSECA, ANA LUCIA. In vitro toxicity of isolated strains and cyanobacterial bloom biomasses over Paramecium caudatum (ciliophora): Lessons from a non-metazoan model organism. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 202, OCT 1 2020. (14/50420-9, 18/01563-2)

JONES, MARTIN R.; PINTO, ERNANI; TORRES, MARIANA A.; DORR, FABIANE; MAZUR-MARZEC, HANNA; SZUBERT, KAROLINA; TARTAGLIONE, LUCIANA; DELL'AVERSANO, CARMELA; MILES, CHRISTOPHER O.; BEACH, DANIEL G.; et al. CyanoMetDB, a comprehensive public database of secondary metabolites from cyanobacteria. WATER RESEARCH, v. 196, MAY 15 2021. (14/50420-9)

KAZUMI K. TERAMOTO; FABIANE DORR; MIRIAM SANZ; ERNANI PINTO. MÉTODO DE TRIAGEM DE MICROGININAS EM CIANOBACTÉRIAS POR LC-MS/MS. Química Nova, v. 43, n. 10, p. 1385-1392, 2021-02-22. (14/50420-9, 13/07914-8)

MIRANDA LOPES, TACIANA ONESORGE; PASSOS, LARISSA SOUZA; VIEIRA, LUIZA VALLI; PINTO, ERNANI; DORR, FABIANE; SCHERER, RODRIGO; SALUSTRIANO, NATHACHA DE ANDRADE; WEITZEL DIAS CARNEIRO, MARIA TEREZA; POSTAY, LAIS FRIGINI; GOMES, LEVY CARVALHO. Metals, arsenic, pesticides, and microcystins in tilapia (Oreochromis niloticus) from aquaculture parks in Brazil. Environmental Science and Pollution Research, v. 27, n. 16, p. 14-pg., 2020-04-01. (14/50420-9)

MIRANDA LOPES, TACIANA ONESORGE; PASSOS, LARISSA SOUZA; VIEIRA, LUIZA VALLI; PINTO, ERNANI; DORR, FABIANE; SCHERER, RODRIGO; SALUSTRIANO, NATHACHA DE ANDRADE; WEITZEL DIAS CARNEIRO, MARIA TEREZA; POSTAY, LAIS FRIGINI; GOMES, LEVY CARVALHO. Metals, arsenic, pesticides, and microcystins in tilapia (Oreochromis niloticus) from aquaculture parks in Brazil. Environmental Science and Pollution Research, v. 27, n. 16, SI, APR 2020. (14/50420-9)

GERALDES, VANESSA; JACINAVICIUS, FERNANDA RIOS; GENUARIO, DIEGO BONALDO; PINTO, ERNANI. Identification and distribution of mycosporine-like amino acids in Brazilian cyanobacteria using ultrahigh-performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, FEB 2020. (14/50420-9, 14/26131-7)

GUIMARAES, NATALIA RODRIGUES; DORR, FABIANE; MARQUES, RODRIGO DE OLIVEIRA; PINTO, ERNANI; FERREIRA FILHO, SIDNEY SECKLER. Removal efficiency of dissolved organic matter from secondary effluent by coagulation-flocculation processes. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, v. 56, n. 2, p. 161-170, DEC 14 2020. (12/04464-9, 14/50420-9, 13/07914-8)

FERNANDES, KELLY A.; DORR, FELIPE AUGUSTO; PINTO, ERNANI. Stability Analyses by HPLC-MS of Guanitoxin Isolated from Sphaerospermopsis torques-reginae. Journal of the Brazilian Chemical Society, v. 32, n. 8, p. 1559-1567, AUG 2021. (14/50420-9)

POPIN, RAFAEL VICENTINI; DELBAJE, ENDREWS; DE ABREU, VINICIUS AUGUSTO CARVALHO; RIGONATO, JANAINA; DORR, FELIPE AUGUSTO; PINTO, ERNANI; SIVONEN, KAARINA; FIORE, MARLI FATIMA. Genomic and Metabolomic Analyses of Natural Products in Nodularia spumigena Isolated from a Shrimp Culture Pond. TOXINS, v. 12, n. 3, MAR 2020. (13/50425-8, 14/50420-9)

GERALDES, VANESSA; DE MEDEIROS, LIVIA S.; JACINAVICIUS, FERNANDA R.; LONG, PAUL F.; PINTO, ERNANI. Development and validation of a rapid LC-MS/MS method for the quantification of mycosporines and mycosporine-like amino acids (MAAs) from cyanobacteria. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 46, MAR 2020. (15/22742-4, 14/50420-9)

LIMA, STELLA T.; ALYARENGA, DANILLO.; ETCHEGARAY, AUGUSTO; FEWER, DAVID P.; JOKELA, JOUNI; VARANI, ALESSANDRO M.; SANZ, MIRIAM; DORR, FELIPE A.; PINTO, ERNANI; SIYONEN, KAARINA; et al. Genetic Organization of Anabaenopeptin and Spumigin Biosynthetic Gene Clusters in the Cyanobacterium Sphaerospermopsis torquesreginae ITEP-024. ACS Chemical Biology, v. 12, n. 3, p. 769-778, MAR 2017. (12/25272-0, 14/50420-9, 11/08092-6, 13/50425-8)

SANZ, MIRIAM; SALINAS, ROBERTO KOPKE; PINTO, ERNANI. Namalides B and C and Spumigins K-N from the Cultured Freshwater Cyanobacterium Sphaerospermopsis torques-reginae. Journal of Natural Products, v. 80, n. 9, p. 2492-2501, SEP 2017. (14/50420-9, 12/25272-0, 16/07490-1)

DOS SANTOS, DOUGLAS SILVA; ROSA, MARIA EDUARDA; ZANATTA, ANA PAULA; OLIVEIR, RAQUEL SOARES; MOREIRA DE ALMEIDA, CARLOS GABRIEL; LEAL, ALLAN PINTO; SANZ, MIRIAM; FERNANDES, KELLY AFONSINA; DE SOUZA, VELCI QUEIROZ; DE ASSIS, DENIS REIS; et al. Neurotoxic effects of sublethal concentrations of cyanobacterial extract containing anatoxin-a(s) on Nauphoeta cinerea cockroaches. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 171, p. 138-145, APR 30 2019. (14/50420-9)

GERALDES, VANESSA; DE MEDEIROS, LIVIA SOMAN; LIMA, STELLA T.; ALVARENGA, DANILLO OLIVEIRA; GACESA, RANKO; LONG, PAUL F.; FIORE, MARLI FATIMA; PINTO, ERNANI. Genetic and biochemical evidence for redundant pathways leading to mycosporine-like amino acid biosynthesis in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. ALGAE, v. 35, n. 2, p. 177-187, JUN 2020. (14/50420-9, 15/22742-4, 18/01563-2)

GONZALEZ-BLANCO, CARLOS; DORR, FELIPE AUGUSTO; ALBUQUERQUE, RENATA; ONUKI, JANICE; PINTO, ERNANI. Alternative Isolation Protocol for Desulfo and Zwitterionic Cylindrospermopsin Alkaloids and Comparison of Their Toxicity in HepG2 Cells. Molecules, v. 25, n. 13, JUL 2020. (14/50420-9)

GERALDES, VANESSA; JACINAVICIUS, FERNANDA RIOS; GENUARIO, DIEGO BONALDO; PINTO, ERNANI. Identification and distribution of mycosporine-like amino acids in Brazilian cyanobacteria using ultrahigh-performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, p. 10-pg., 2020-02-08. (14/50420-9, 14/26131-7)

BINZER, SOFIE BJORNHOLT; SVENSSEN, DANIEL KILLERUP; DAUGBJERG, NIELS; ALVES-DE-SOUZA, CATHARINE; PINTO, ERNANI; HANSEN, PER JUEL; LARSEN, THOMAS OSTENFELD; VARGA, ELISABETH. A-, B- and C-type prymnesins are clade specific compounds and chemotaxonomic markers in Prymnesium parvum. HARMFUL ALGAE, v. 81, p. 8-pg., 2019-01-01. (14/50420-9)