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Evaluation of salivary composition and masticatory function in overweight and obese adolescents

Abstract

Obesity is a chronic multifactorial disease associated with an inflammatory condition of low intensity. It is considered a public health problem in developed and developing countries assuming epidemic proportions. It is assumed that changes in individual's oral specific characteristics, such as biochemistry and salivary microbiological compositions, may be related to inflammatory status, nutritional deficiency and dyslipidemia associated with overweight. The masticatory function may have an impact on nutritional status, although this issue has been rarely addressed in the literature. Thus, the aim of this study is to evaluate the biochemical composition in terms of salivary pH and flow rate, cholesterol, 7 ketocholesterol, 25-hydroxyvitamin D2 and D3, uric acid, expression of nuclear factor kappa ² (marker of inflammation), salivary microbiota and quality of the masticatory function of 220 adolescents of both genders aged between 14 and 17 years of public schools in Piracicaba (SP). The physical examination will involve measurements of height, weight, fat mass and skeletal muscle mass through a digital stadiometer and bioelectrical impedance analysis to divide the subjects in the following groups: normal-weight, overweight and obesity, according to reference BMI-for-age and sex (5-19 years) (WHO, 2007). By means of oral examination, the inclusion criteria will be evaluated: presence of permanent dentition without dental caries and absence of periodontal pockets (>3mm). Stimulated and non- stimulated saliva will be collected in schools, in the morning, for the determination of cholesterol, 7- ketocholesterol, uric acid and 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 by high performance liquid chromatography (HPLC). The evaluation of NF- kappa ² expression will be assessed by real-time PCR or quantitative PCR (qPCR). For microbiological analysis, salivary DNA samples will be isolated and subjected to quantitative PCR reactions (qPCR) in order to determine the proportion of Selenomonas noxia, Bifidobacteria, Streptococcus mutans and Prevotella intermedia in relation to the total bacterial load. The evaluation of masticatory function will be performed by objective and subjective methods, namely: Orofacial Myofunctional Evaluation expanded protocol, masticatory performance with colorimetric method, electromyographic activity of masseter and temporalis muscles, maximum unilateral bite force and subjective assessment of quality of masticatory function using an specific questionnaire. The description of the studied variables and the correlation will be analyzed using descriptive statistics, normality tests, correlation tests (Pearson or Spearman) and unpaired t test or Mann-Whitney, where appropriate. A multiple logistic regression model will be used to examine the association of studied variables with weight excess (±=0.05), controlling for potential confounding factors. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ARAUJO, DARLLE SANTOS; KLEIN, MARLISE INEZ; SCUDINE, KELLY GUEDES DE OLIVEIRA; DE SALES LEITE, LUANA; PARISOTTO, THAIS M.; FERREIRA, CAROLINE MARCANTONIO; FONSECA, FERNANDO LUIZ AFFONSO; PEREZ, MATHEUS MOREIRA; CASTELO, PAULA MIDORI. Salivary Microbiological and Gingival Health Status Evaluation of Adolescents With Overweight and Obesity: A Cluster Analysis. FRONTIERS IN PEDIATRICS, v. 8, JUL 31 2020. Web of Science Citations: 0.
ARAUJO, DARLLE SANTOS; DE OLIVEIRA SCUDINE, KELLY GUEDES; PEDRONI-PEREIRA, ALINE; DUARTE GAVIAO, MARIA BEATRIZ; PEREIRA, EDIMAR CRISTIANO; AFFONSO FONSECA, FERNANDO LUIZ; CASTELO, PAULA MIDORI. Salivary uric acid is a predictive marker of body fat percentage in adolescents. Nutrition Research, v. 74, p. 62-70, FEB 2020. Web of Science Citations: 0.
DE ANDRADE, PEDRO AVELINO MAIA; GIOVANI, PRISCILA ALVES; ARAUJO, DARLLE SANTOS; DE SOUZA, ADIJAILTON JOSE; PEDRONI-PEREIRA, ALINE; KANTOVITZ, KAMILA ROSAMILIA; ANDREOTE, FERNANDO DINI; CASTELO, PAULA MIDORI; NOCITI-JR, FRANCISCO HUMBERTO. Shifts in the bacterial community of saliva give insights on the relationship between obesity and oral microbiota in adolescents. Archives of Microbiology, v. 202, n. 5 FEB 2020. Web of Science Citations: 0.
PEDRONI-PEREIRA, ALINE; SALOME MARQUEZIN, MARIA CAROLINA; ARAUJO, DARLLE SANTOS; PEREIRA, LUCIANO JOSE; BOMMARITO, SILVANA; CASTELO, PAULA MIDORI. Lack of agreement between objective and subjective measures in the evaluation of masticatory function: A preliminary study. Physiology & Behavior, v. 184, p. 220-225, FEB 1 2018. Web of Science Citations: 4.
PEDRONI-PEREIRA, ALINE; ARAUJO, DARLLE SANTOS; DE OLIVEIRA SCUDINE, KELLY GUEDES; DE ALMEIDA PRADO, DANIELA GALVAO; NUNES LEITE LIMA, DEBORA ALVES; CASTELO, PAULA MIDORI. Chewing in adolescents with overweight and obesity: An exploratory study with behavioral approach. APPETITE, v. 107, p. 527-533, DEC 1 2016. Web of Science Citations: 1.
DE OLIVEIRA SCUDINE, KELLY GUEDES; PEDRONI-PEREIRA, ALINE; ARAUJO, DARLLE SANTOS; DE ALMEIDA PRADO, DANIELA GALVAO; ROSSI, ANA CLAUDIA; CASTELO, PAULA MIDORI. Assessment of the differences in masticatory behavior between male and female adolescents. Physiology & Behavior, v. 163, p. 115-122, SEP 1 2016. Web of Science Citations: 8.
MARIBEL HILASACA-MAMANI; TAÍS DE SOUZA BARBOSA; CLAUDIA FEGADOLLI; PAULA MIDORI CASTELO. Validity and reliability of the quality of masticatory function questionnaire applied in Brazilian adolescents. CoDAS, v. 28, n. 2, p. 149-154, Abr. 2016. Web of Science Citations: 3.

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