Abstract
This thematic Project includes 29 subprojects, focusing on emerging zoonotic viruses of the family Bunyaviridae, distributed in 3 genera: Orthobunyavirus, Phlebovirus and Hantavirus. The project involves 3 Principal Researchers, 55 Brazilian and international collaborators, and will take 4 years. For studies on the genus Orthobunyavirus, serological diagnosis of Oropouche virus (OROV) by ELISA will be developed using a recombinant N protein of OROV as antigen. This will enable sero-epidemiological surveys in patients, horses, small mammals and birds. The recombinant N protein of OROV will also be tested as an immunogen in mice, and the mechanism of its apoptotic effect will be studied in vitro. The antiviral activity of 10,000 compounds for Orthobunyavirus will be tested. Cell biology in vitro studies will be done to verify sites of OROV assembly and budding in host cells, and to identify factors that regulate intracellular traffic of proteins involved in those processes. A fluorescent OROV will also be produced by reverse genetics for in vitro and in vivo studies. Molecular epidemiology and phylogenetic studies of orthobunyaviruses based on complete genomes will be done by next-generation sequencing. A real time RT-PCR assay for detection of Brazilian orthobunyaviruses of serogroups C and Simbu will also be developed. Studies on the genus Phlebovirus will include molecular epidemiology and phylogeny, development of a real time RT-PCR to assess their occurrence in clinical samples from patients with acute febrile illness, testing of the antiviral activity of of 10,000 compounds and development of an experimental mouse model of infection by Candiru virus. Studies will also be done to detect assembly/budding sites of Candiru virus. Studies on the genus Hantavirus will include virus detection in hantavirus cardio-pulmonary syndrome (HCPS) and study of its pathophysiology based on levels of cytokines in patient blood samples. Importantly, we plan to characterize genetically and morphologically the Araraquara (ARQV) strain of antavirus and evaluate the immunogenicity of a recombinant N protein of ARQV in mice. We also will test the antiviral activity of 10,000 compounds against The strain Rio Mamore of hantavirus). Field capture of small mammals and their ectoparasites will be done to investigate Hantavirus infection, especially in Necromys lasiurus. A molecular epidemiology study will be done in cooperation with scientists from other national and international (USA, Finland and Colombia) institutions, based on computational analysis of the evolution and geographical distribution of Brazilian hantaviruses. Cell biology studies will hopefully reveal details of Hantavirus assembly and budding. (AU)
Scientific publications
(16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RUSSO, RAQUEL RINALDI;
DOS SANTOS JUNIOR, NILTON NASCIMENTO;
OLIVEIRA CINTRA, ADELIA CRISTINA;
MORAES FIGUEIREDO, LUIZ TADEU;
SAMPAIO, SUELY VILELA;
AQUINO, VICTOR HUGO.
Expression, purification and virucidal activity of two recombinant isoforms of phospholipase A(2) from Crotalus durissus terrificus venom.
ARCHIVES OF VIROLOGY,
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n. 4,
p. 1159-1171,
APR 2019.
Web of Science Citations: 0.
DE SOUZA, WILLIAM MARCIE;
FUMAGALLI, MARCILIO JORGE;
TORRES CARRASCO, ADRIANO DE OLIVEIRA;
ROMEIRO, MARILIA FARIGNOLI;
MODHA, SEJAL;
SEKI, MEIRE CHRISTINA;
GHELLER, JANAINA MENEGAZZO;
DAFFRE, SIRLEI;
TEIXEIRA NUNES, MARCIO ROBERTO;
MURCIA, PABLO RAMIRO;
ACRANI, GUSTAVO OLSZANSKI;
MORAES FIGUEIREDO, LUIZ TADEU.
Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil.
SCIENTIFIC REPORTS,
v. 8,
NOV 5 2018.
Web of Science Citations: 2.
DASILVA, LUIS L. P.
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FRONTIERS IN MICROBIOLOGY,
v. 9,
OCT 10 2018.
Web of Science Citations: 0.
FUMAGALLI, MARCILIO JORGE;
DE SOUZA, WILLIAM MARCIEL;
ALVES ESPOSITO, DANILLO LUCAS;
SILVA, ANGELICA;
ROMEIRO, MARILIA FARIGNOLI;
MARTINEZ, EDSON ZANGIACOMI;
LOPES DA FONSECA, BENEDITO ANTONIO;
MORAES FIGUEIREDO, LUIZ TADEU.
Enzyme-linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus.
VIROLOGY JOURNAL,
v. 15,
JUL 24 2018.
Web of Science Citations: 1.
AMARILLA, ALBERTO ANASTACIO;
FUMAGALLI, MARCILIO JORGE;
FIGUEIREDO, MARIO LUIS;
LIMA-JUNIOR, DJALMA S.;
SANTOS-JUNIOR, NILTON NASCIMENTO;
ALFONSO, HELDA LIZ;
LIPPI, VERONICA;
TRABUCO, AMANDA CRISTINA;
LAURETTI, FLAVIO;
MULLER, VANESSA DANIELLE;
COLON, DAVID F.;
LUIZ, JOAO P. M.;
SUHRBIER, ANDREAS;
SETOH, YIN XIANG;
KHROMYKH, ALEXANDER A.;
MORAES FIGUEIREDO, LUIZ TADEU;
AQUINO, VICTOR HUGO.
Ilheus and Saint Louis encephalitis viruses elicit cross-protection against a lethal Rocio virus challenge in mice.
PLoS One,
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JUN 13 2018.
Web of Science Citations: 0.
SABINO-SANTOS, JR., GILBERTO;
MOTTA MAIA, FELIPE GONCALVES;
MARTINS, RONALDO BRAGANCA;
GAGLIARDI, TALITA BIANCA;
DE SOUZA, WILLIAM MARCIEL;
MUYLAERT, RENATA LARA;
DE SOUZA LUNA, LUCIANO KLEBER;
MELO, DANILO MACHADO;
CARDOSO, RICARDO DE SOUZA;
BARBOSA, NATALIA DA SILVA;
PONTELLI, MARJORIE CORNEJO;
MAMANI-ZAPANA, PRISCILA ROSSE;
VIEIRA, THALLYTA MARIA;
MELO, NORMA MARIA;
JONSSON, COLLEEN B.;
GOODIN, DOUGLAS;
SALAZAR-BRAVO, JORGE;
PINTO DASILVA, LUIS LAMBERTI;
ARRUDA, EURICO;
MORAES FIGUEIREDO, LUIZ TADEU.
Natural infection of Neotropical bats with hantavirus in Brazil.
SCIENTIFIC REPORTS,
v. 8,
JUN 13 2018.
Web of Science Citations: 0.
DE MELO JUNIOR, ARISTIDES BEZERRA;
DE SOUZA, WILLIAM MARCIEL;
ACRANI, GUSTAVO OLSZANSKI;
CARVALHO, VALERIA LIMA;
ROMEIRO, MARILIA FARIGNOLI;
TOLARDO, ALINE LAVADO;
DA SILVA, SANDRO PATROCA;
CARDOSO, JEDSON FERREIRA;
CHIANG, JANNIFER DE OLIVEIRA;
DA SILVA GONCALVES VIANEZ JUNIOR, JOAO LIDIO;
DA SILVA AZEVEDO, RAIMUNDA DO SOCORRO;
MORAES FIGUEIREDO, LUIZ TADEU;
DA COSTA VASCONCELOS, PEDRO FERNANDO;
TEIXEIRA NUNES, MARCIO ROBERTO;
DE ALMEIDA MEDEIROS, DANIELE BARBOSA.
Genomic characterization and evolution of Tacaiuma orthobunyavirus (Peribunyaviridae family) isolated in Brazil.
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v. 60,
p. 71-76,
JUN 2018.
Web of Science Citations: 0.
BARBOSA, NATALIA S.;
MENDONCA, LEILA R.;
DIAS, MARCOS V. S.;
PONTELLI, MARJORIE C.;
DA SILVA, ELAINE Z. M.;
CRIADO, MIRIA F.;
DA SILVA-JANUARIO, MARA E.;
SCHINDLER, MICHAEL;
JAMUR, MARIA C.;
OLIVER, CONSTANCE;
ARRUDA, EURICO;
DASILVA, LUIS L. P.
ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments.
PLOS PATHOGENS,
v. 14,
n. 5
MAY 2018.
Web of Science Citations: 0.
COSTA BARREIRA, LUZIA APARECIDA;
SCHEUCHER, PRISCILA SANTOS;
ROMEIRO, MARILIA FARIGNOLI;
LA SERRA, LEONARDO;
BADRA, SORAYA JABUR;
DE SOUZA, WILLIAM MARCIEL;
MORAES FIGUEIREDO, LUIZ TADEU.
Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells.
Revista da Sociedade Brasileira de Medicina Tropical,
v. 51,
n. 2,
p. 168-173,
MAR-APR 2018.
Web of Science Citations: 1.
NUNES-NETO, JOAQUIM PINTO;
DE SOUZA, WILLIAM MARCIEL;
ACRANI, GUSTAVO OLSZANSKI;
ROMEIRO, MARILIA FARIGNOLI;
FUMAGALLI, MARCILIOJORGE;
VIEIRA, LUIZ CARLOS;
DE ALMEIDA MEDEIROS, DANIELE BARBOSA;
LIMA, JULIANA ABREU;
SILVA DE LIMA, CLAYTON PEREIRA;
CARDOSO, JEDSON FERREIRA;
MORAES FIGUEIREDO, LUIZ TADEU;
DA SILVA, SANDRO PATROCA;
TESH, ROBERT;
TEIXEIRA NUNES, MARCIO ROBERTO;
DA COSTA VASCONCELOS, PEDRO FERNANDO.
Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil.
JOURNAL OF GENERAL VIROLOGY,
v. 98,
n. 4,
p. 585-594,
APR 2017.
Web of Science Citations: 2.
TAVARES, LUCAS A.;
DA SILVA, EULALIA M. L.;
DA SILVA-JANUARIO, MARA E.;
JANUARIO, YUNAN C.;
DE CAVALHO, JULIANNE V.;
CZERNISZ, ERIKA S.;
MARDONES, GONZALO A.;
DASILVA, LUIS L. P.
CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the gamma 1 and gamma 2 subunits of the AP-1 complex in protein trafficking.
Journal of Cell Science,
v. 130,
n. 2,
p. 429-443,
JAN 15 2017.
Web of Science Citations: 7.
DE SOUZA, WILLIAM MARCIEL;
ACRANI, GUSTAVO OLSZANSKI;
ROMEIRO, MARILIA FARIGNOLI;
REIS JUNIOR, OSVALDO;
TOLARDO, ALINE LAVADO;
SERRAO DE ANDRADE, AMANDA ARAUJO;
GONCALVES VIANEZ JUNIOR, JOAO LIDIO DA SILVA;
DE ALMEIDA MEDEIROS, DANIELE BARBOSA;
TEIXEIRA NUNES, MARCIO ROBERTO;
MORAES FIGUEIREDO, LUIZ TADEU.
Complete genome sequence of Piry vesiculovirus.
ARCHIVES OF VIROLOGY,
v. 161,
n. 8,
p. 2325-2328,
AUG 2016.
Web of Science Citations: 0.
DE SOUZA LUNA, LUCIANO KLEBER;
RODRIGUES, ALCIR HUMBERTO;
MARQUES SANTOS, RODRIGO IVO;
SESTI-COSTA, RENATA;
CRIADO, MIRIA FERREIRA;
MARTINS, RONALDO B.;
SILVA, MARIA LUCIA;
DELCARO, LUANA SELLA;
PROENCA-MODENA, JOSE LUIZ;
MORAES FIGUEIREDO, LUIZ TADEU;
ACRANI, GUSTAVO OLSZANSKI;
ARRUDA, EURICO.
Oropouche virus is detected in peripheral blood leukocytes from patients.
Journal of Medical Virology,
v. 89,
n. 6,
p. 1108-1111,
JUN 2016.
Web of Science Citations: 5.
DE SOUZA, WILLIAM MARCIEL;
ACRANI, GUSTAVO OLSZANSKI;
ROMEIRO, MARILIA FARIGNOLI;
REIS, JR., OSVALDO;
TOLARDO, ALINE LAVADO;
DA SILVA, SANDRO PATROCA;
DE ALMEIDA MEDEIROS, DANIELE BARBOSA;
VARELA, MARIANA;
TEIXEIRA NUNES, MARCIO ROBERTO;
MORAES FIGUEIREDO, LUIZ TADEU.
Molecular characterization of Capim and Enseada orthobunyaviruses.
INFECTION GENETICS AND EVOLUTION,
v. 40,
p. 47-53,
JUN 2016.
Web of Science Citations: 6.