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Trypanosoma Cruzi signaling due to changes in the external conditions: ECM and pH changes

Grant number: 14/25494-9
Support type:Regular Research Grants
Duration: March 01, 2015 - August 31, 2017
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Maria Julia Manso Alves
Grantee:Maria Julia Manso Alves
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Herein we propose to identify posttranslational modifications of proteins from T. cruzi in two distinct conditions, both relevants for the infection step: adhesion to extracellular matrix and exposure to mild pH decrease (pH 6). Levels of phosphorylation and S-nitrosylation of proteins will be determined in both conditions, with special attention to the flagellar proteins, since flagellum is a sensor element of the external medium and is lost in late amastigogenesis Proteins associated with the paraflagellar rod will be pull down by anti-PAR antibodies and identified. Identification of these proteins by MS/MS, as well as the possible involvement of NO/ NOS in this event will be pursued. Since S-nitrosylation (as well as others NO-dependent modifications) is related to the levels of NO, the activity of NO synthase and levels of cAMP and cGMP will be determined. The goal of the project is to understand the modifications at the molecular level induced in T. cruzi as a consequence of the interation with the extracellular matrix and lower pH, both essential conditions for the establishment of the parasite infection. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MATTOS, ELICIANE C.; CANUTO, GISELE; MANCHOLA, NUBIA C.; MAGALHAES, RUBENS D. M.; CROZIER, THOMAS W. M.; LAMONT, DOUGLAS J.; TAVARES, MARINA F. M.; COLLI, WALTER; FERGUSON, MICHAEL A. J.; ALVES, MARIA JULIA M. Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix. PLoS Neglected Tropical Diseases, v. 13, n. 2 FEB 2019. Web of Science Citations: 1.
BAPTISTA CANUTO, GISELE ANDRE; DORR, FABIANE; GHILARDI LAGO, JOAO HENRIQUE; TEMPONE, ANDRE GUSTAVO; PINTO, ERNANI; PIMENTA, DANIEL CARVALHO; SIMON FARAH, JOAO PEDRO; MANSO ALVES, MARIA JULIA; MAGGI TAVARES, MARINA FRANCO. New insights into the mechanistic action of methyldehydrodieugenol B towards Leishmania (L.) infantum via a multiplatform based untargeted metabolomics approach. METABOLOMICS, v. 13, n. 5 MAY 2017. Web of Science Citations: 2.
MANSO ALVES, MARIA JULIA; KAWAHARA, REBECA; VINER, ROSA; COLLI, WALTER; MATTOS, ELICIANE CEVOLANI; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN ROSSEL; PALMISANO, GIUSEPPE. Comprehensive glycoprofiling of the epimastigote and trypomastigote stages of Trypanosoma cruzi. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 182-192, JAN 16 2017. Web of Science Citations: 17.

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