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Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models

Grant number: 14/06923-6
Support type:Program for Research on Bioenergy (BIOEN) - Thematic Grants
Duration: April 01, 2015 - March 31, 2019
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Andre Luis Ferraz
Grantee:Andre Luis Ferraz
Home Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
Co-Principal Investigators:Adriane Maria Ferreira Milagres
Assoc. researchers:Elisson Antonio da Costa Romanel ; Fábio Márcio Squina ; Fernando Segato ; Júlio César dos Santos ; Marina Oliveira de Souza Dias ; Sergio Almeida Pacca
Associated scholarship(s):15/23279-6 - Metagenomic and metasecretomic analyses of an enrichment microbial consortium of anaerobic fungi to the degradation of sugarcane bagasse: prospection of cellulosomas and lignocellolytic enzymes, BP.PD
16/02985-2 - Evaluation of the correlation between sucrose accumulation process in sugarcane and the digestibility of lignocellulosic biomass, BP.IC
16/01926-2 - Influence of an accessory domain in biochemical and structural characteristics of the cellulase CelE2, BP.MS
15/23684-8 - Evaluation of a process involving sugarcane fractionation in rind and inner parts prior to the pre-treatment and enzymatic hydrolysis, BP.IC
15/10756-0 - Development of biorefinery models using experimental sugarcane hybrids and energy cane with different pretreatment methods, BP.PD

Abstract

The project will provide integrated knowledge related to the sugar cane cell wall recalcitrance. Based on this knowledge, innovative biorefinery models will be proposed for the conversion of the sugar cane biomass into valuable products. Secondary cell wall formation will be evaluated along complete maturation cycles experimental hybrids that contrast for lignocellulose composition. UV- and fluorescence-microscopy will be used to evaluate the lignification and the hemicellulose deposition processes, respectively. Expression of monolignols biosynthesis genes will be evaluated as well. Studied samples will be evaluated also according to their digestibility by hydrolytic enzymes. Data compilation will give a general view of the cell wall formation in sugar cane and how deposition of the cellulose-encapsulating agents (lignin, hydroxicinnamic acids and hemicellulose) affects the biomass recalcitrance. New biorefinery models for sugar cane biomass conversion will be designed with basis on the accumulated knowledge. It is advanced that mild pretreatment processes based on the action of alkaline-sulfite or diluted acids will provide substrates susceptible for xylanase pre-digestion that could provide valuable hemicellulose olygosaccharides. After this step, the remaining substrate would be susceptible for efficient cellulose hydrolysis by cellulases, producing fermentable sugars. New hydrolytic enzymes identified with basis on the secretome study of wood decay fungi will be tested for the hydrolysis of specific pretreated materials. A final evaluation of the designed processes with basis on life cycle assessment will indicate which benefits could be obtained by the use of low-recalcitrance plants pretreated by specially designed pretreatment steps (AU)

Scientific publications (25)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COLLUCCI, DANIEL; BUENO, RAPHAEL C. A.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. Sucrose content, lignocellulose accumulation and in vitro digestibility of sugarcane internodes depicted in relation to internode maturation stage and Saccharum genotypes. INDUSTRIAL CROPS AND PRODUCTS, v. 139, NOV 1 2019. Web of Science Citations: 0.
VALADARES, FERNANDA; GONCALVES, THIAGO A.; DAMASIO, ANDRE; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; SEGATO, FERNANDO; FERRAZ, ANDRE. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Enzyme and Microbial Technology, v. 130, NOV 2019. Web of Science Citations: 0.
BATISTA, DIEGO SILVA; KOEHLER, ANDREA DIAS; ROMANEL, ELISSON; DE SOUZA, VINICIUS CAIRUS; SILVA, TATIANE DULCINEIA; ALMEIDA, MAIRA CAROLINA; MACIEL, TALLES ELISSON F.; BUENO FERREIRA, PERACIO RAFAEL; SOUSA FELIPE, SERGIO HEITOR; SALDANHA, CLEBER WITT; MALDANER, JOSEILA; CARNEVALLI DIAS, LEONARDO LUCAS; FESTUCCI-BUSELLI, REGINALDO ALVES; OTONI, WAGNER CAMPOS. De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production. PROTOPLASMA, v. 256, n. 3, p. 601-614, MAY 2019. Web of Science Citations: 2.
SANTOS, MAIARA P.; REINOSO, FELIPE A. M.; TAVILLA, VERONICA; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F. On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 18, MAR 2019. Web of Science Citations: 0.
BERTO, GABRIELA LEILA; VELASCO, JOSMAN; CABOS RIBEIRO, CAIO TASSO; ZANPHORLIN, LETICIA MARIA; DOMINGUES, MARIANE NORONHA; MURAKAMI, MARIO TYAGO; POLIKARPOV, IGOR; DE OLIVEIRA, LEANDRO CRISTANTE; FERRAZ, ANDRE; SEGATO, FERNANDO. Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45. Enzyme and Microbial Technology, v. 120, p. 23-35, JAN 2019. Web of Science Citations: 2.
MENDES, F. M.; VASCONCELOS, M. H.; DIAS, M. O. S.; FERRAZ, A.; MILAGRES, A. M. F.; SANTOS, J. C.; JESUS, C. D. F.; WATANABE, M. D. B.; JUNQUEIRA, T. L.; BONOMI, A. Alkaline sulfite pretreatment for integrated first and second generation ethanol production: A techno-economic assessment of sugarcane hybrids. BIOMASS & BIOENERGY, v. 119, p. 314-321, DEC 2018. Web of Science Citations: 2.
MONTE, JOSEANA R.; LAURITO-FRIEND, DEBORA F.; MUSSATTO, I, SOLANGE; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F. Comparative Evaluation of Acid and Alkaline Sulfite Pretreatments for Enzymatic Saccharification of Bagasses from Three Different Sugarcane Hybrids. BIOTECHNOLOGY PROGRESS, v. 34, n. 4, p. 944-951, JUL-AUG 2018. Web of Science Citations: 1.
REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; MILAGRES, ADRIANE M. F.; DEL RIO, JOSE C.; FERRAZ, ANDRE. Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse. BIOTECHNOLOGY FOR BIOFUELS, v. 11, JUN 5 2018. Web of Science Citations: 3.
MAINARDI, PEDRO H.; FEITOSA, VALKER A.; BRENELLI DE PAIVA, LIVIA B.; BONUGLI-SANTOS, RAFAELLA C.; SQUINA, FABIO M.; PESSOA, JR., ADALBERTO; SETTE, LARA D. Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063. FUNGAL BIOLOGY, v. 122, n. 5, p. 302-309, MAY 2018. Web of Science Citations: 4.
MAMEDES-RODRIGUES, T. C.; BATISTA, D. S.; NAPOLEAO, T. A.; CRUZ, A. C. F.; FORTINI, E. A.; NOGUEIRA, F. T. S.; ROMANEL, E.; OTONI, W. C. Lignin and cellulose synthesis and antioxidative defense mechanisms are affected by light quality in Brachypodium distachyon. PLANT CELL TISSUE AND ORGAN CULTURE, v. 133, n. 1, p. 1-14, APR 2018. Web of Science Citations: 6.
DE OLIVEIRA SANTOS, VICTOR TABOSA; SIQUEIRA, GERMANO; FERREIRA MILAGRES, ADRIANE MARIA; FERRAZ, ANDRE. Role of hemicellulose removal during dilute acid pretreatment on the cellulose accessibility and enzymatic hydrolysis of compositionally diverse sugarcane hybrids. INDUSTRIAL CROPS AND PRODUCTS, v. 111, p. 722-730, JAN 2018. Web of Science Citations: 11.
SPORCK, DANIELE; REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; DEL RIO, JOSE C.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F. Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches. BIOTECHNOLOGY FOR BIOFUELS, v. 10, DEC 7 2017. Web of Science Citations: 7.
MENDES, F. M.; DIAS, M. O. S.; FERRAZ, A.; MILAGRES, A. M. F.; SANTOS, J. C.; BONOMI, A. Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity. CHEMICAL ENGINEERING RESEARCH & DESIGN, v. 125, p. 72-78, SEP 2017. Web of Science Citations: 5.
SIQUEIRA, GERMANO; ARANTES, VALDEIR; SADDLER, JACK N.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F. Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin. BIOTECHNOLOGY FOR BIOFUELS, v. 10, JUL 11 2017. Web of Science Citations: 19.
PIMENTEL, AGNES C.; EMATSU, GABRIELA C. G.; LIBERATO, MARCELO V.; PAIXAO, DOUGLAS A. A.; FRANCO CAIRO, JOAO PAULO L.; MANDELLI, FERNANDA; TRAMONTINA, ROBSON; GANDIN, CESAR A.; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO M.; ALVAREZ, THABATA M. Biochemical and biophysical properties of a metagenome-derived GH5 endoglucanase displaying an unconventional domain architecture. International Journal of Biological Macromolecules, v. 99, p. 384-393, JUN 2017. Web of Science Citations: 4.
DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; BATTAGLIA, EVY; BAYRAM, OZGUR; BENOCCI, TIZIANO; BRAUS-STROMEYER, SUSANNA A.; CALDANA, CAMILA; CANOVAS, DAVID; CERQUEIRA, GUSTAVO C.; CHEN, FUSHENG; CHEN, WANPING; CHOI, CINDY; CLUM, ALICIA; CORREA DOS SANTOS, RENATO AUGUSTO; DE LIMA DAMASIO, ANDRE RICARDO; DIALLINAS, GEORGE; EMRI, TAMAS; FEKETE, ERZSEBET; FLIPPHI, MICHEL; FREYBERG, SUSANNE; GALLO, ANTONIA; GOURNAS, CHRISTOS; HABGOOD, ROB; HAINAUT, MATTHIEU; LAURA HARISPE, MARIA; HENRISSAT, BERNARD; HILDEN, KRISTIINA S.; HOPE, RYAN; HOSSAIN, ABEER; KARABIKA, EUGENIA; KARAFFA, LEVENTE; KARANYI, ZSOLT; KRASEVEC, NADA; KUO, ALAN; KUSCH, HARALD; LABUTTI, KURT; LAGENDIJK, ELLEN L.; LAPIDUS, ALLA; LEVASSEUR, ANTHONY; LINDQUIST, ERIKA; LIPZEN, ANNA; LOGRIECO, ANTONIO F.; MACCABE, ANDREW; MAKELA, MIIA R.; MALAVAZI, IRAN; MELIN, PETTER; MEYER, VERA; MIELNICHUK, NATALIA; MISKEI, MARTON; MOLNAR, AKOS P.; MULE, GIUSEPPINA; NGAN, CHEW YEE; OREJAS, MARGARITA; OROSZ, ERZSEBET; OUEDRAOGO, JEAN PAUL; OVERKAMP, KARIN M.; PARK, HEE-SOO; PERRONE, GIANCARLO; PIUMI, FRANCOIS; PUNT, PETER J.; RAM, ARTHUR F. J.; RAMON, ANA; RAUSCHER, STEFAN; RECORD, ERIC; RIANO-PACHON, DIEGO MAURICIO; ROBERT, VINCENT; ROEHRIG, JULIAN; RULLER, ROBERTO; SALAMOV, ASAF; SALIH, NADHIRA S.; SAMSON, ROB A.; SANDOR, ERZSEBET; SANGUINETTI, MANUEL; SCHUTZE, TABEA; SEPCIC, KRISTINA; SHELEST, EKATERINA; SHERLOCK, GAVIN; SOPHIANOPOULOU, VICKY; SQUINA, FABIOM.; SUN, HUI; SUSCA, ANTONIA; TODD, RICHARD B.; TSANG, ADRIAN; UNKLES, SHIELA E.; VAN DE WIELE, NATHALIE; VAN ROSSEN-UFFINK, DIANA; DE CASTRO OLIVEIRA, JULIANA VELASCO; VESTH, TAMMI C.; VISSER, JAAP; YU, JAE-HYUK; ZHOU, MIAOMIAO; ANDERSEN, MIKAEL R.; ARCHER, DAVID B.; BAKER, SCOTT E.; BENOIT, ISABELLE; BRAKHAGE, AXEL A.; BRAUS, GERHARD H.; FISCHER, REINHARD; FRISVAD, JENS C.; GOLDMAN, GUSTAVO H.; HOUBRAKEN, JOS; OAKLEY, BERL; POCSI, ISTVAN; SCAZZOCCHIO, CLAUDIO; SEIBOTH, BERNHARD; VANKUYK, PATRICIA A.; WORTMAN, JENNIFER; DYER, PAUL S.; GRIGORIEV, IGOR V. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biology, v. 18, FEB 14 2017. Web of Science Citations: 94.
MACHADO, ANGELA DA SILVA; FERRAZ, ANDRE. Biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation. Bioresource Technology, v. 225, p. 17-22, FEB 2017. Web of Science Citations: 18.
CALZADO, FELIPE; PRATES, ERICA T.; GONCALVES, THIAGO A.; RUBIO, MARCELO V.; ZUBIETA, MARIANE P.; SQUINA, FABIO M.; SKAF, MUNIR S.; DAMASIO, ANDRE R. L. Molecular basis of substrate recognition and specificity revealed in family 12 glycoside hydrolases. Biotechnology and Bioengineering, v. 113, n. 12, p. 2577-2586, DEC 2016. Web of Science Citations: 4.
CESARINO, IGOR; SIMOES, MARCELLA SIQUEIRA; BRITO, MICHAEL DOS SANTOS; FANELLI, AMANDA; SILVA, TATIANE DA FRANCA; ROMANEL, ELISSON. Building the wall: recent advances in understanding lignin metabolism in grasses. ACTA PHYSIOLOGIAE PLANTARUM, v. 38, n. 11 NOV 2016. Web of Science Citations: 10.
RUBIO, MARCELO VENTURA; ZUBIETA, MARIANE PALUDETTI; LOURENO FRANCO CAIRO, JOAO PAULO; CALZADO, FELIPE; PAES LEME, ADRIANA FRANCO; SQUINA, FABIO MARCIO; PRADE, ROLF ALEXANDER; DE LIMA DAMASIO, ANDRE RICARDO. Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose. BIOTECHNOLOGY FOR BIOFUELS, v. 9, AUG 8 2016. Web of Science Citations: 9.
VALADARES, FERNANDA; GONCALVES, THIAGO A.; GONCALVES, DAYELLE S. P. O.; SEGATO, FERNANDO; ROMANEL, ELISSON; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; FERRAZ, ANDRE. Exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 23 2016. Web of Science Citations: 17.
COSTA, THALES H. F.; VEGA-SANCHEZ, MIGUEL E.; MILAGRES, ADRIANE M. F.; SCHELLER, HENRIK V.; FERRAZ, ANDRE. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 4 2016. Web of Science Citations: 14.
HAHN SCHNEIDER, WILLIAN DANIEL; GONCALVES, THIAGO AUGUSTO; UCHIMA, CRISTIANE AKEMI; COUGER, MATTHEW BRIAN; PRADE, ROLF; SQUINA, FABIO MARCIO; PINHEIRO DILLON, ALDO JOSE; CAMASSOLA, MARLI. Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAR 17 2016. Web of Science Citations: 24.
MESQUITA, JESSICA FARIA; FERRAZ, ANDRE; AGUIAR, ANDRE. Alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse. Bioprocess and Biosystems Engineering, v. 39, n. 3, p. 441-448, MAR 2016. Web of Science Citations: 18.
MENDES, FERNANDA M.; FONSECA, MARIANA B.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F. Anatomic and Ultrastructural Characteristics of Different Regions of Sugar Cane Internodes Which Affect Their Response to Alkaline Sulfite Pretreatment and Material Recalcitrance. ENERGY & FUELS, v. 30, n. 2, p. 1078-1084, FEB 2016. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180168185 - Universidade de São Paulo (USP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - August 2018, 16