Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models

• Grant number: 14/06923-6
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Start date: April 01, 2015
• End date: March 31, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Andre Luis Ferraz
• Grantee: Andre Luis Ferraz
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Pesquisadores principais: Adriane Maria Ferreira Milagres
• Associated researchers: Elisson Antonio da Costa Romanel ; Fábio Márcio Squina ; Fernando Segato ; Júlio César dos Santos ; Marina Oliveira de Souza Dias ; Sergio Almeida Pacca
• Associated scholarship(s): 15/23279-6 - Metagenomic and metasecretomic analyses of an enrichment microbial consortium of anaerobic fungi to the degradation of sugarcane bagasse: prospection of cellulosomas and lignocellolytic enzymes, BP.PD; 16/02985-2 - Evaluation of the correlation between sucrose accumulation process in sugarcane and the digestibility of lignocellulosic biomass, BP.IC; 16/01926-2 - Influence of an accessory domain in biochemical and structural characteristics of the cellulase CelE2, BP.MS; 15/23684-8 - Evaluation of a process involving sugarcane fractionation in rind and inner parts prior to the pre-treatment and enzymatic hydrolysis, BP.IC; 15/10756-0 - Development of biorefinery models using experimental sugarcane hybrids and energy cane with different pretreatment methods, BP.PD

Abstract

The project will provide integrated knowledge related to the sugar cane cell wall recalcitrance. Based on this knowledge, innovative biorefinery models will be proposed for the conversion of the sugar cane biomass into valuable products. Secondary cell wall formation will be evaluated along complete maturation cycles experimental hybrids that contrast for lignocellulose composition. UV- and fluorescence-microscopy will be used to evaluate the lignification and the hemicellulose deposition processes, respectively. Expression of monolignols biosynthesis genes will be evaluated as well. Studied samples will be evaluated also according to their digestibility by hydrolytic enzymes. Data compilation will give a general view of the cell wall formation in sugar cane and how deposition of the cellulose-encapsulating agents (lignin, hydroxicinnamic acids and hemicellulose) affects the biomass recalcitrance. New biorefinery models for sugar cane biomass conversion will be designed with basis on the accumulated knowledge. It is advanced that mild pretreatment processes based on the action of alkaline-sulfite or diluted acids will provide substrates susceptible for xylanase pre-digestion that could provide valuable hemicellulose olygosaccharides. After this step, the remaining substrate would be susceptible for efficient cellulose hydrolysis by cellulases, producing fermentable sugars. New hydrolytic enzymes identified with basis on the secretome study of wood decay fungi will be tested for the hydrolysis of specific pretreated materials. A final evaluation of the designed processes with basis on life cycle assessment will indicate which benefits could be obtained by the use of low-recalcitrance plants pretreated by specially designed pretreatment steps (AU)

Scientific publications

(35)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biology, v. 18, FEB 14 2017. (14/06923-6, 12/20549-4, 12/19040-0, 11/08945-9, 14/10351-8, 14/11766-7)

SIQUEIRA, GERMANO; ARANTES, VALDEIR; SADDLER, JACK N.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin. BIOTECHNOLOGY FOR BIOFUELS, v. 10, JUL 11 2017. (12/09940-3, 14/06923-6, 08/56256-5)

VALADARES, FERNANDA; GONCALVES, THIAGO A.; GONCALVES, DAYELLE S. P. O.; SEGATO, FERNANDO; ROMANEL, ELISSON; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; FERRAZ, ANDRE. Exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 23 2016. (14/06923-6, 08/56256-5)

COSTA, THALES H. F.; VEGA-SANCHEZ, MIGUEL E.; MILAGRES, ADRIANE M. F.; SCHELLER, HENRIK V.; FERRAZ, ANDRE. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAY 4 2016. (14/06923-6, 11/50535-2, 08/56256-5)

MENDES, F. M.; DIAS, M. O. S.; FERRAZ, A.; MILAGRES, A. M. F.; SANTOS, J. C.; BONOMI, A.. Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity. CHEMICAL ENGINEERING RESEARCH & DESIGN, v. 125, p. 72-78, SEP 2017. (14/06923-6, 15/10756-0)

CESARINO, IGOR; SIMOES, MARCELLA SIQUEIRA; BRITO, MICHAEL DOS SANTOS; FANELLI, AMANDA; SILVA, TATIANE DA FRANCA; ROMANEL, ELISSON. Building the wall: recent advances in understanding lignin metabolism in grasses. ACTA PHYSIOLOGIAE PLANTARUM, v. 38, n. 11, NOV 2016. (14/06923-6, 15/02527-1, 14/23017-9, 14/17486-6, 14/08468-4, 15/18361-5)

RUBIO, MARCELO VENTURA; ZUBIETA, MARIANE PALUDETTI; LOURENO FRANCO CAIRO, JOAO PAULO; CALZADO, FELIPE; PAES LEME, ADRIANA FRANCO; SQUINA, FABIO MARCIO; PRADE, ROLF ALEXANDER; DE LIMA DAMASIO, ANDRE RICARDO. Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose. BIOTECHNOLOGY FOR BIOFUELS, v. 9, AUG 8 2016. (14/15403-6, 14/06923-6, 11/20977-3, 13/24988-5, 12/20549-4, 14/23051-2, 09/54067-3)

MESQUITA, JESSICA FARIA; FERRAZ, ANDRE; AGUIAR, ANDRE. Alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse. Bioprocess and Biosystems Engineering, v. 39, n. 3, p. 441-448, MAR 2016. (14/06923-6, 08/56256-5)

SARTORI, GUILHERME P.; VASCONCELOS, MARCELO H.; SANTOS, JULIO C.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. An innovative concept for industrial sugarcane processing enhances polysaccharide utilization in first- and second-generation integrated biorefineries. INDUSTRIAL CROPS AND PRODUCTS, v. 141, DEC 1 2019. (15/23684-8, 14/06923-6)

CORNETTI, ALINE A. A.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Enzyme-aided xylan extraction from alkaline-sulfite pretreated sugarcane bagasse and its incorporation onto eucalyptus kraft pulps. Carbohydrate Research, v. 492, JUN 2020. (14/06923-6)

DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. GENOME BIOLOGY, v. 18, p. 45-pg., 2017-02-14. (11/08945-9, 14/06923-6, 12/19040-0, 14/10351-8, 14/11766-7, 12/20549-4)

VELASCO, JOSMAN; OLIVA, BIANCA; GONCALVES, ALINE LARISSA; LIMA, AWANA SILVA; FERREIRA, GISLENE; FRANCA, BRUNO ALVES; MULINARI, EVANDRO JOSE; GONCALVES, THIAGO AUGUSTO; SQUINA, FABIO MARCIO; SEIKI KADOWAKI, MARCO ANTONIO; et al. Functional characterization of a novel thermophilic exo-arabinanase fromThermothielavioides terrestris. Applied Microbiology and Biotechnology, v. 104, n. 19, AUG 2020. (14/18714-2, 17/16089-1, 14/06923-6, 19/01165-0, 15/50590-4, 19/06663-8)

COLLUCCI, DANIEL; BUENO, RAPHAEL C. A.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. Sucrose content, lignocellulose accumulation and in vitro digestibility of sugarcane internodes depicted in relation to internode maturation stage and Saccharum genotypes. INDUSTRIAL CROPS AND PRODUCTS, v. 139, NOV 1 2019. (16/02985-2, 14/06923-6)

RAMOS, LUCAS; VASCONCELOS, MARCELO H.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE; DIAS, MARINA O. S.; MENDES, FERNANDA M.; DOS SANTOS, JULIO C.. High-solid enzymatic hydrolysis of sugarcane bagasse and ethanol production in repeated batch process using column reactors. 3 BIOTECH, v. 11, n. 10, OCT 2021. (15/10756-0, 14/06923-6)

VALADARES, FERNANDA; GONCALVES, THIAGO A.; DAMASIO, ANDRE; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; SEGATO, FERNANDO; FERRAZ, ANDRE. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Enzyme and Microbial Technology, v. 130, NOV 2019. (17/16089-1, 15/50590-4, 14/06923-6)

DE OLIVEIRA SANTOS, VICTOR TABOSA; SIQUEIRA, GERMANO; FERREIRA MILAGRES, ADRIANE MARIA; FERRAZ, ANDRE. Role of hemicellulose removal during dilute acid pretreatment on the cellulose accessibility and enzymatic hydrolysis of compositionally diverse sugarcane hybrids. INDUSTRIAL CROPS AND PRODUCTS, v. 111, p. 722-730, JAN 2018. (14/06923-6)

SPORCK, DANIELE; REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; DEL RIO, JOSE C.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches. BIOTECHNOLOGY FOR BIOFUELS, v. 10, DEC 7 2017. (14/06923-6, 08/56256-5)

MACHADO, ANGELA DA SILVA; FERRAZ, ANDRE. Biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation. Bioresource Technology, v. 225, p. 17-22, FEB 2017. (14/06923-6, 08/56256-5)

REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; MILAGRES, ADRIANE M. F.; DEL RIO, JOSE C.; FERRAZ, ANDRE. Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse. BIOTECHNOLOGY FOR BIOFUELS, v. 11, JUN 5 2018. (14/06923-6)

MAMEDES-RODRIGUES, T. C.; BATISTA, D. S.; NAPOLEAO, T. A.; CRUZ, A. C. F.; FORTINI, E. A.; NOGUEIRA, F. T. S.; ROMANEL, E.; OTONI, W. C.. Lignin and cellulose synthesis and antioxidative defense mechanisms are affected by light quality in Brachypodium distachyon. PLANT CELL TISSUE AND ORGAN CULTURE, v. 133, n. 1, p. 1-14, APR 2018. (14/06923-6, 14/17486-6, 16/24391-7)

MACHADO, ANGELA S.; VALADARES, FERNANDA; SILVA, TATIANE F.; MILAGRES, ADRIANE M. F.; SEGATO, FERNANDO; FERRAZ, ANDRE. The Secretome ofPhanerochaete chrysosporiumandTrametes versicolorGrown in Microcrystalline Cellulose and Use of the Enzymes for Hydrolysis of Lignocellulosic Materials. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, JUL 17 2020. (14/18714-2, 14/06923-6)

VELASCO, JOSMAN; PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; SEPULCHRO, ANA GABRIELA VEIGA; KADOWAKI, MARCO ANTONIO SEIKI; SANTO, MELISSA CRISTINA ESPIRITO; POLIKARPOV, IGOR; SEGATO, FERNANDO. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification. Enzyme and Microbial Technology, v. 144, MAR 2021. (19/22284-7, 15/13684-0, 14/06923-6, 14/18714-2, 19/01165-0, 17/50025-0)

ABDELLA, ASMAA; SEGATO, FERNANDO; WILKINS, MARK R.. Optimization of nutrient medium components for production of a client endo-beta-1,4-xylanase from Aspergillus fumigatus var. niveus using a recombinant Aspergillus nidulans strain. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 20, JUL 2019. (14/06923-6, 14/18714-2)

MENDES, F. M.; VASCONCELOS, M. H.; DIAS, M. O. S.; FERRAZ, A.; MILAGRES, A. M. F.; SANTOS, J. C.; JESUS, C. D. F.; WATANABE, M. D. B.; JUNQUEIRA, T. L.; BONOMI, A.. Alkaline sulfite pretreatment for integrated first and second generation ethanol production: A techno-economic assessment of sugarcane hybrids. BIOMASS & BIOENERGY, v. 119, p. 314-321, DEC 2018. (15/10756-0, 14/06923-6)

MONTE, JOSEANA R.; LAURITO-FRIEND, DEBORA F.; MUSSATTO, I, SOLANGE; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Comparative Evaluation of Acid and Alkaline Sulfite Pretreatments for Enzymatic Saccharification of Bagasses from Three Different Sugarcane Hybrids. BIOTECHNOLOGY PROGRESS, v. 34, n. 4, p. 944-951, JUL-AUG 2018. (14/06923-6)

SANTOS, MAIARA P.; REINOSO, FELIPE A. M.; TAVILLA, VERONICA; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 18, MAR 2019. (14/06923-6)

VASCONCELOS, MARCELO HOLANDA; MENDES, FERNANDA MACHADO; RAMOS, LUCAS; DIAS, MARINA OLIVEIRA S.; BONOMI, ANTONIO; JESUS, CHARLES DAYAN F.; WATANABE, MARCOS DJUN B.; JUNQUEIRA, TASSIA LOPES; MILAGRES, ADRIANE MARIA F.; FERRAZ, ANDRE; et al. Techno-economic assessment of bioenergy and biofuel production in integrated sugarcane biorefinery: Identification of technological bottlenecks and economic feasibility of dilute acid pretreatment. ENERGY, v. 199, MAY 15 2020. (15/10756-0, 14/06923-6)

FANELLI, AMANDA; REINHARDT, LAURIE; MATSUOKA, SIZUO; FERRAZ, ANDRE; SILVA, TATIANE DA FRANCA; HATFIELD, RONALD D.; ROMANEL, ELISSON. Biomass composition of two new energy cane cultivars compared with their ancestral Saccharum spontaneum during internode development. BIOMASS & BIOENERGY, v. 141, OCT 2020. (14/06923-6, 14/17486-6, 16/24391-7, 16/25785-9)

MENDES, FERNANDA M.; FONSECA, MARIANA B.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Anatomic and Ultrastructural Characteristics of Different Regions of Sugar Cane Internodes Which Affect Their Response to Alkaline Sulfite Pretreatment and Material Recalcitrance. ENERGY & FUELS, v. 30, n. 2, p. 1078-1084, FEB 2016. (14/06923-6, 11/50535-2, 08/56256-5)

MAINARDI, PEDRO H.; FEITOSA, VALKER A.; BRENELLI DE PAIVA, LIVIA B.; BONUGLI-SANTOS, RAFAELLA C.; SQUINA, FABIO M.; PESSOA, JR., ADALBERTO; SETTE, LARA D.. Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063. FUNGAL BIOLOGY, v. 122, n. 5, p. 302-309, MAY 2018. (14/06923-6, 13/12505-0, 13/03061-0, 16/07957-7, 13/19486-0)

HAHN SCHNEIDER, WILLIAN DANIEL; GONCALVES, THIAGO AUGUSTO; UCHIMA, CRISTIANE AKEMI; COUGER, MATTHEW BRIAN; PRADE, ROLF; SQUINA, FABIO MARCIO; PINHEIRO DILLON, ALDO JOSE; CAMASSOLA, MARLI. Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAR 17 2016. (14/06923-6, 08/58037-9, 12/19040-0)

CALZADO, FELIPE; PRATES, ERICA T.; GONCALVES, THIAGO A.; RUBIO, MARCELO V.; ZUBIETA, MARIANE P.; SQUINA, FABIO M.; SKAF, MUNIR S.; DAMASIO, ANDRE R. L.. Molecular basis of substrate recognition and specificity revealed in family 12 glycoside hydrolases. Biotechnology and Bioengineering, v. 113, n. 12, p. 2577-2586, DEC 2016. (13/08293-7, 14/06923-6, 12/20549-4, 13/24988-5, 14/23051-2)

PIMENTEL, AGNES C.; EMATSU, GABRIELA C. G.; LIBERATO, MARCELO V.; PAIXAO, DOUGLAS A. A.; FRANCO CAIRO, JOAO PAULO L.; MANDELLI, FERNANDA; TRAMONTINA, ROBSON; GANDIN, CESAR A.; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO M.; et al. Biochemical and biophysical properties of a metagenome-derived GH5 endoglucanase displaying an unconventional domain architecture. International Journal of Biological Macromolecules, v. 99, p. 384-393, JUN 2017. (14/06923-6, 14/04105-4, 16/01926-2, 10/11469-1, 14/12861-3, 08/58037-9)

BERTO, GABRIELA LEILA; VELASCO, JOSMAN; CABOS RIBEIRO, CAIO TASSO; ZANPHORLIN, LETICIA MARIA; DOMINGUES, MARIANE NORONHA; MURAKAMI, MARIO TYAGO; POLIKARPOV, IGOR; DE OLIVEIRA, LEANDRO CRISTANTE; FERRAZ, ANDRE; SEGATO, FERNANDO. Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45. Enzyme and Microbial Technology, v. 120, p. 23-35, JAN 2019. (14/06923-6, 14/18714-2, 15/13684-0, 15/26982-0)

BATISTA, DIEGO SILVA; KOEHLER, ANDREA DIAS; ROMANEL, ELISSON; DE SOUZA, VINICIUS CAIRUS; SILVA, TATIANE DULCINEIA; ALMEIDA, MAIRA CAROLINA; MACIEL, TALLES ELISSON F.; BUENO FERREIRA, PERACIO RAFAEL; SOUSA FELIPE, SERGIO HEITOR; SALDANHA, CLEBER WITT; et al. De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production. PROTOPLASMA, v. 256, n. 3, p. 601-614, MAY 2019. (14/06923-6, 14/17486-6)

Filed patent(s) as a result of this research project