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Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications

Grant number: 14/06863-3
Support type:Research Grants - Young Investigators Grants
Duration: February 01, 2015 - September 30, 2019
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Giuseppe Palmisano
Grantee:Giuseppe Palmisano
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Cláudio Romero Farias Marinho ; Fernando Augusto Soares ; Vilma Regina Martins
Associated grant(s):18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications, AP.JP2
18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins, AP.EMU
16/50356-4 - MYC: a regulator of exosome protein cargo? , AP.R
Associated scholarship(s):19/01798-2 - Implementation and optimization of computational platforms for expanding the proteome data analysis and OMICs data integration, BP.TT
18/13283-4 - Discovery of biomarkers of Chagas' disease in urine using mass spectrometry techniques, BP.DR
18/12349-1 - Post-translation modification in cancer and infectious diseases diagnostics: methodological approaches and biological applications, BP.TT
16/23689-2 - Proteomic and post-translation modification characterization of Leishmanias L. (V.) braziliensis, L. (L.) amazonensis and L. (L.) chagasi using a shotgun protemics approaches., BP.MS
15/02866-0 - Discovery and verification of intact glycopeptides as candidate prostate cancer biomarkers using mass spectrometry-based proteomics approaches, BP.PD

Abstract

An innovative research program will be established aiming at studying protein post-translational modifications (PTMs), such as phosphorylation, glycosylation and oxidation modulation in pathological conditions such as cancer metastasis and parasite infection diagnosis. The research will be articulated on three lines: 1) Optimize and develop mass spectrometry-based enrichment/analysis methods for PTMs studies in different biological systems; 2) Develop a strategy for the recovery and analysis of secreted modified proteins and peptides originating from interstitial fluid from dissected prostate cancers and surrounding healthy tissues; 3) Establish a mass spectrometry-based method to detect biomarkers of parasite infection in biofluids in order to develop a point-of-care diagnostic platform capable of detecting these diseases at an early stage. (AU)

Scientific publications (21)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; EVEREST-DASS, ARUN; FOERSTER, SARAH; HINNEBURG, HANNES; LEITE, KATIA R. M.; LOKE, IAN; MOGINGER, UWE; MOH, EDWARD S. X.; NAKANO, MIYAKO; RECUERO, SAULO; SETHI, MANVEEN K.; SROUGI, MIGUEL; STAVENHAGEN, KATHRIN; VENKATAKRISHNAN, VIGNESH; WONGTRAKUL-KISH, KATHERINE; DIESTEL, SIMONE; HOFFMANN, PETER; KARLSSON, NICLAS G.; KOLARICH, DANIEL; MOLLOY, MARK P.; MUDERS, MICHAEL H.; OEHLER, MARTIN K.; PACKER, NICOLLE H.; PALMISANO, GIUSEPPE; THAYSEN-ANDERSEN, MORTEN. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, SI NOV 2019. Web of Science Citations: 1.
KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, SI NOV 2019. Web of Science Citations: 0.
FUZITA, FELIPE J.; PIMENTA, DANIEL C.; PALMISANO, GIUSEPPE; TERRA, WALTER R.; FERREIRA, CLELIA. Detergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretion. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 235, p. 8-18, SEP 2019. Web of Science Citations: 0.
CAPELLI-PEIXOTO, JANAINA; MULE, SIMON NGAO; TANO, FABIA TOMIE; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteomics and Leishmaniasis: Potential Clinical Applications. PROTEOMICS CLINICAL APPLICATIONS, v. 13, n. 6 AUG 2019. Web of Science Citations: 0.
ROSA-FERNANDES, LIVIA; CUGOLA, FERNANDA RODRIGUES; RUSSO, FABIELE BALDINO; KAWAHARA, REBECA; DE MELO FREIRE, CAIO CESAR; CORREA LEITE, PAULO EMLIO; BASSI STERN, ANA CAROLINA; ANGELI, CLAUDIA BLANES; LEAL DE OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; DE ANDRADE ZANOTTO, PAOLO MARINHO; DURIGON, EDISON LUIZ; LARSEN, MARTIN ROSSEL; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA; PALMISANO, GIUSEPPE. Zika Virus Impairs Neurogenesis and Synaptogenesis Pathways in Human Neural Stem Cells and Neurons. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 13, MAR 15 2019. Web of Science Citations: 1.
PEREIRA, ADRIANA S. A.; AMARAL, MURILO S.; VASCONCELOS, ELTON J. R.; PIRES, DAVID S.; ASIF, HUMA; DASILVA, LUCAS F.; MORALES-VICENTE, DAVID A.; CARNEIRO, VITOR C.; ANGELI, CLAUDIA B.; PALMISANO, GIUSEPPE; FANTAPPIE, MARCELO R.; PIERCE, RAYMOND J.; SETUBAL, JOAO C.; VERJOVSKI-ALMEIDA, SERGIO. Inhibition of histone methyltransferase EZH2 in Schistosoma mansoni in vitro by GSK343 reduces egg laying and decreases the expression of genes implicated in DNA replication and noncoding RNA metabolism. PLoS Neglected Tropical Diseases, v. 12, n. 10 OCT 2018. Web of Science Citations: 2.
CARREGARI, VICTOR CORASSOLLA; ROSA-FERNANDES, LIVIA; BALDASSO, PAULO; BYDLOWSKI, SERGIO PAULO; MARANGONI, SERGIO; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Snake Venom Extracellular vesicles (SVEVs) reveal wide molecular and functional proteome diversity. SCIENTIFIC REPORTS, v. 8, AUG 13 2018. Web of Science Citations: 2.
AVILA, CARLA CRISTI; MULE, SIMON NGAO; ROSA-FERNANDES, LIVIA; VINER, ROSA; BARISON, MARIA JULIA; COSTA-MARTINS, ANDRE GUILLHERME; DE OLIVEIRA, GILBERTO SANTOS; GERALDES TEIXEIRA, MARTA MARIA; FARIAS MARINHO, CLAUDIO ROMERO; SILBER, ARIEL MARIANO; PALMISANO, GIUSEPPE. Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation. GENES, v. 9, n. 8 AUG 2018. Web of Science Citations: 1.
DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; AVILA, CARLA CRISTI; TEIXEIRA, MARTA M. G.; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Development of a Trypanosoma cruzi strain typing assay using MS2 peptide spectral libraries (Tc-STAMS2). PLoS Neglected Tropical Diseases, v. 12, n. 4 APR 2018. Web of Science Citations: 3.
RAMIREZ, MARCEL I.; AMORIM, MARIA G.; GADELHA, CATARINA; MILIC, IVANA; WELSH, JOSHUA A.; FREITAS, VANESSA M.; NAWAZ, MUHAMMAD; AKBAR, NAVEED; COUCH, YVONNE; MAKIN, LAURA; COOKE, FIONA; VETTORE, ANDRE L.; BATISTA, PATRICIA X.; FREEZOR, ROBERTA; PEZUK, JULIA A.; ROSA-FERNANDES, LIVIA; CARREIRA, ANA CLAUDIA O.; DEVITT, ANDREW; JACOBS, LAURA; SILVA, ISRAEL T.; COAKLEY, GILLIAN; NUNES, DIANA N.; CARTER, DAVE; PALMISANO, GIUSEPPE; DIAS-NETO, EMMANUEL. Technical challenges of working with extracellular vesicles. NANOSCALE, v. 10, n. 3, p. 881-906, JAN 21 2018. Web of Science Citations: 60.
ROSA-FERNANDES, LIVIA; ROCHA, VICTORIA BOMBARDA; CARREGARI, VICTOR CORASOLLA; URBANI, ANDREA; PALMISANO, GIUSEPPE. A Perspective on Extracellular Vesicles Proteomics. FRONTIERS IN CHEMISTRY, v. 5, NOV 21 2017. Web of Science Citations: 23.
ROSA-FERNANDES, LIVIA; MASELLI, LUCIANA M. P.; MAEDA, NAIR Y.; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO P. Outside-in, inside-out: Proteomic analysis of endothelial stress mediated by 7-ketocholesterol. Chemistry and Physics of Lipids, v. 207, n. B, p. 231-238, OCT 2017. Web of Science Citations: 7.
CIREGIA, FEDERICA; URBANI, ANDREA; PALMISANO, GIUSEPPE. Extracellular Vesicles in Brain Tumors and Neurodegenerative Diseases. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 10, AUG 31 2017. Web of Science Citations: 21.
DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; AVILA, CARLA C.; LARSEN, MARTIN R.; PEREIRA ALVES, JOAO MARCELO; PALMISANO, GIUSEPPE. Novel DNA coding regions and protein arginylation reveal unexplored T. cruzi proteome and PTMs. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, n. SI, p. 51-66, JUL 2017. Web of Science Citations: 1.
PIMENTEL, ANDRE C.; FUZITA, FELIPE J.; PALMISANO, GIUSEPPE; FERREIRA, CELIA; TERRA, WALTER R. Role of cathepsins D in the midgut of Dysdercus peruvianus. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 204, p. 45-52, FEB 2017. Web of Science Citations: 9.
FERNANDES, LIVIA ROSA; BASSI STERN, ANA CAROLINA; CAVAGLIERI, RITA DE CASSIA; SOUSA NOGUEIRA, FABIO CESAR; DOMONT, GILBERTO; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO PAULO. 7-Ketocholesterol overcomes drug resistance in chronic myeloid leukemia cell lines beyond MDRI mechanism. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 12-23, JAN 16 2017. Web of Science Citations: 5.
MANSO ALVES, MARIA JULIA; KAWAHARA, REBECA; VINER, ROSA; COLLI, WALTER; MATTOS, ELICIANE CEVOLANI; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN ROSSEL; PALMISANO, GIUSEPPE. Comprehensive glycoprofiling of the epimastigote and trypomastigote stages of Trypanosoma cruzi. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 182-192, JAN 16 2017. Web of Science Citations: 17.
KAWAHARA, REBECA; SAAD, JOYCE; ANGELI, CLAUDIA BLANES; PALMISANO, GIUSEPPE. Site-specific characterization of N-linked glycosylation in human urinary glycoproteins and endogenous glycopeptides. GLYCOCONJUGATE JOURNAL, v. 33, n. 6, p. 937-951, DEC 2016. Web of Science Citations: 5.
HUANG, HONGGANG; PETERSEN, MARTIN HAAR; IBANEZ-VEA, MARIA; LASSEN, PERNILLE S.; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. MOLECULAR & CELLULAR PROTEOMICS, v. 15, n. 10, p. 3282-3296, OCT 2016. Web of Science Citations: 15.
BOYSEN, ANDERS; PALMISANO, GIUSEPPE; KROGH, THOGER JENSEN; DUGGIN, IAIN G.; LARSEN, MARTIN R.; MOLLER-JENSEN, JAKOB. A novel mass spectrometric strategy ``BEMAP{''} reveals Extensive O-linked protein glycosylation in Enterotoxigenic Escherichia coli. SCIENTIFIC REPORTS, v. 6, AUG 26 2016. Web of Science Citations: 6.
AVILA, C. C.; ALMEIDA, F. G.; PALMISANO, G. Direct identification of trypanosomatids by matrix-assisted laser desorption ionization-time of flight mass spectrometry (DIT MALDI-TOF MS). Journal of Mass Spectrometry, v. 51, n. 8, p. 549-557, AUG 2016. Web of Science Citations: 6.

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