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Quantification of endotoxins and its relationship with signs / symptoms and volumetric lesion periapical in primary endodontic infection


The objectives of the present study are the following: a) to quantify endotoxins and bacterial load in primary endodontic infections before and after biomechanical preparation (BMP) and use of different intracanal medications (ICMs) in the case of multiple-session therapy; b) to relate levels of endotoxins and cultivable microorganisms to periapical lesion volume by using cone beam computed tomography (CBCT), thus assessing possible relationships with clinical signs and symptoms; c) to relate the same levels to root canal volume; d) to compare the volume of periapical lesions before and after 1 year of endodontic treatment in cases of single-session and multiple-session therapy. Forty teeth with pulp necrosis and periapical lesion will be selected for study, all being submitted to CBCT. After coronal opening, an initial collection will be performed to assess the presence of root canal infection. Next, endodontic treatment will be performed by using 2.5% NaOCl and the teeth divided into 4 groups according to the treatment protocol as follows: GI - Ca(OH)2: calcium hydroxide P.A. + saline solution; GII - Ca(OH)2 + CLX: calcium hydroxide P.A. + 2% chlorhexidine; GIII - Ultracal: calcium hydroxide paste (Ultracal ®); and GIV - SS: single-session therapy. Root canal samples will be collected after BMP and after 14 days of ICM (in case of multiple-session therapy). Antimicrobial activity tests for microbiological culture will be performed in all samples, including analysis of endotoxin quantification by using limulus amebocyte lysate assay. Volumetric analysis of periapical bone destruction and root canals will be performed by using CBCT and Nemotec ® software. In addition, endodontic treatment management will be also conducted by comparing the periapical lesion volume before and after 1 year of different treatment protocols. All data will be statistically analysed. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAVALLI, DAIANA; TOIA, CASSIA CESTARI; FLORES OROZCO, ESTEBAN ISAI; KHOURY, RAYANA DUARTE; DA ROSA CARDOSO, FLAVIA GOULART; ALVES, MARCELO CORREA; TALGE CARVALHO, CLAUDIO ANTONIO; VALERA, MARCIA CARNEIRO. Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study. JOURNAL OF ENDODONTICS, v. 43, n. 8, p. 1237-1245, . (15/05397-1, 14/25789-9)
FLÁVIA GOULART DA ROSA CARDOSO; FREDERICO CANATO MARTINHO; NÁDIA DE SOUZA FERREIRA; RENATA FALCHETE DO PRADO; LUIZ ROBERTO COUTINHO MANHÃES-JÚNIOR; MARCO ANTÔNIO ROCCO; MARCIA CARNEIRO VALERA. Correlation Between Volume of Root Canal, Cultivable Bacteria, Bacterial Complexes and Endotoxins in Primary Infection. Brazilian Dental Journal, v. 30, n. 2, p. 117-122, . (14/25789-9)
TOIA, CASSIA CESTARI; KHOURY, RAYANA DUARTE; CORAZZA, BRUNA JORDAO MOTTA; OROZCO, ESTEBAN ISAI FLORES; VALERA, MARCIA CARNEIRO. Effectiveness of 1-Visit and Retreatment of Teeth with Persistent/Secondary Endodontic Infection: A Randomized Clinical Trial with 18 Months of Follow-up. JOURNAL OF ENDODONTICS, v. 48, n. 1, p. 4-14, . (14/25789-9, 18/01703-9)
ESTEBAN ISAI FLORES OROZCO; CASSIA CESTARI TOIA; DAIANA CAVALLI; RAYANA DUARTE KHOURY; FLÁVIA GOULART DA ROSA CARDOSO; EDUARDO BRESCIANI; MARCIA CARNEIRO VALERA. Effect of passive ultrasonic activation on microorganisms in primary root canal infection: a randomized clinical trial. Journal of Applied Oral Science, v. 28, . (14/25789-9)
DE CARVALHO BAPTISTA, IVANY MACHADO; MARTINHO, FREDERICO CANATO; NASCIMENTO, GUSTAVO GIACOMELLI; DA ROCHA SANTOS, CARLOS EDUARDO; DO PRADO, RENATA FALCHETE; VALERA, MARCIA CARNEIRO. Colonization of oropharynx and lower respiratory tract in critical patients: Risk of ventilator-associated pneumonia. ARCHIVES OF ORAL BIOLOGY, v. 85, p. 64-69, . (14/25789-9)
FELIPE DE SOUZA MATOS; RAYANA DUARTE KHOURY; CLÁUDIO ANTONIO TALGE CARVALHO; FREDERICO CANATO MARTINHO; EDUARDO BRESCIANI; MARCIA CARNEIRO VALERA. Effect of EDTA and QMIX Ultrasonic Activation on the Reduction of Microorganisms and Endotoxins in Ex Vivo Human Root Canals. Brazilian Dental Journal, v. 30, n. 3, p. 220-226, . (14/25789-9)

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