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Effect of protease activated receptor 1 (PAR1) activation on osteogenic activity of periodontal ligament cells

Grant number: 15/07396-2
Support type:Regular Research Grants
Duration: August 01, 2015 - January 31, 2018
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Marinella Holzhausen Caldeira
Grantee:Marinella Holzhausen Caldeira
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Carla Renata Sipert

Abstract

Protease activated receptor 1 (PAR1) activation by thrombin can play an important role on periodontal tissue homeostase and healing. A recent study from our group has shown an increased expression of PAR1 in gingival crevicular fluid cells of chronic periodontitis patients after periodontal treatment, and that PAR1 expression is associated with decreased levels of inflammatory biomarkers. In addition, previous in vitro studies showed that PAR1 activation by thrombin leads to: osteoprotegerin (OPG) production in periodontal ligament cells, a dose-response increase in the proliferation of isolated bone marrow stromal cells of rats, and leads to an increased expression of several growth factors in osteoblasts. Therefore, these studies suggest an important mediator role of PAR1 on periodontal tissue repair. In the present study, we hypothesized that PAR1 activation in periodontal ligament cells can lead to an increased osteogenic activity. Therefore, the main objective of the present study is to evaluate the effect of PAR1 activation by thrombin on the osteogenic differentiation of ligament cells. In addition, the effects of PAR1 activation on mineralized matrix production and on the genetic and proteic expression of alkaline phosphatase, osteopontin, osteocalcin, RANKL, OPG, runt-related transcription factor-2 (Runx2), IL-10 and TGF-ß1 will also be evaluated. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROVAI, EMANUEL SILVA; BATITUCCI AMBROSIO, LUCAS MACEDO; DE FRANCA, BRUNO NUNES; DE OLIVEIRA, LETICIA RODRIGUES; GASPARONI, LETICIA MIQUELITTO; SIPERT, CARLA RENATA; HOLZHAUSEN, MARINELLA. Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells. STEM CELLS INTERNATIONAL, 2019. Web of Science Citations: 0.
ARAUJO, I. M. P.; ALBUQUERQUE-SOUZA, E.; AGUIAR-OLIVEIRA, M. H.; HOLZHAUSEN, M.; OLIVEIRA-NETO, L. A.; SALVATORI, R.; SARAIVA, L.; MAYER, M. P. A.; PANNUTI, C. M.; RIBEIRO, A. O.; ROMITO, G. A.; PUSTIGLIONI, F. E. Immunological and microbiological periodontal profiles in isolated growth hormone deficiency. Journal of Periodontology, v. 89, n. 11, p. 1351-1361, NOV 2018. Web of Science Citations: 1.
LUCAS MACEDO BATITUCCI AMBRÓSIO; EMANUEL DA SILVA ROVAI; BRUNO NUNES DE FRANÇA; DANILO ANDRÉS BALZARINI; IEDA SANTOS ABREU; SHEYLA BATISTA BOLOGNA LOPES; THAÍS BORGUEZAN NUNES; SILVIA VANESSA LOURENÇO; SANDRA GOFINET PASOTO; LUCIANA SARAIVA; MARINELLA HOLZHAUSEN. Effects of periodontal treatment on primary sjȫgren’s syndrome symptoms. Brazilian Oral Research, v. 31, p. -, 2017.
BATITUCCI AMBROSIO, LUCAS MACEDO; ROVAI, EMANUEL DA SILVA; DE FRANCA, BRUNO NUNES; BALZARINI, DANILO ANDRES; ABREU, IEDA SANTOS; BOLOGNA LOPES, SHEYLA BATISTA; NUNES, THAIS BORGUEZAN; LOURENCO, SILVIA VANESSA; PASOTO, SANDRA GOFINET; SARAIVA, LUCIANA; HOLZHAUSEN, MARINELLA. Effects of periodontal treatment on primary sjogren's syndrome symptoms. Brazilian Oral Research, v. 31, 2017. Web of Science Citations: 3.
ROVAI, E. S.; HOLZHAUSEN, M. The Role of Proteinase-Activated Receptors 1 and 2 in the Regulation of Periodontal Tissue Metabolism and Disease. CLINICAL & DEVELOPMENTAL IMMUNOLOGY, 2017. Web of Science Citations: 3.

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