Advanced search
Start date
Betweenand

Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species

Abstract

Spermatogonial Stem Cells (SSCs) are the only stem cells in the body capable to transmit the genetic information from species along generations. This feature is due to the potential of these cells to either self-renew or differentiate to give rise to millions of spermatozoa. Recent studies using SSCs have generated promising methods in the aquaculture, such as the SSC transplantation. This technique consists in the isolation of donor SSCs and their transplantation into a recipient testis, where these cells can develop and produce sperm with donor genetic characteristics. In the current project, we propose a SSC bank which in association with transplantation techniques constitutes a powerful tool to preserve endangered and valuable species. Therefore, the aim of this project is to characterize the molecular markers of SSCs using zebrafish (Danio rerio) as a laboratory model; and to apply this knowledge for a native and high economic interest species, such as jundiá (Rhamdia quelen). Then, SSCs from jundiá will be selected based on these molecular markers, and submitted to cryopreservation techniques afterwards. To test the viability of the cryopreserved cells, SSCs will be thawed and transplanted into recipient testes. In addition, we aimed to generate vasa::gfp jundiás in order to improve the the visualization of the donor-derived spermatogenesis in the recipient testes. This project will provide the basis to create a SSC bank for several teleost species in order to preserve the genotype of endangered and high economic interest species. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINEZ-BENGOCHEA, A.; DORETTO, L.; ROSA, I. F.; OLIVEIRA, M. A.; SILVA, C.; SILVA, D. M. Z. A.; SANTOS, G. R.; SANTOS, J. S. F.; AVELAR, M. M.; SILVA, V, L.; LUCIANELLI-JUNIOR, JR., D.; SOUZA, E. R. B.; SILVA, R. C.; STEWART, A. B.; NAKAGHI, L. S. O.; VALENTIN, F. N.; NOBREGA, R. H. Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 248, OCT-NOV 2020. Web of Science Citations: 0.
XIE, XUAN; NOBREGA, RAFAEL; PSENICKA, MARTIN. Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems. BIOMOLECULES, v. 10, n. 4 APR 2020. Web of Science Citations: 1.
TOVO-NETO, ALDO; MARTINEZ, EMANUEL R. M.; MELO, ALINE G.; DORETTO, LUCAS B.; BUTZGE, ARNO J.; RODRIGUES, MAIRA S.; NAKAJIMA, RAFAEL T.; HABIBI, HAMID R.; NOBREGA, RAFAEL H. Cortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants. BIOMOLECULES, v. 10, n. 3 MAR 2020. Web of Science Citations: 0.
FALLAH, HAMIDEH P.; TOVO-NETO, ALDO; YEUNG, EDWARD C.; NOBREGA, RAFAEL H.; HABIBI, HAMID R. Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone. Molecular and Cellular Endocrinology, v. 492, JUL 15 2019. Web of Science Citations: 1.
DU, KANG; WUERTZ, SVEN; ADOLFI, MATEUS; KNEITZ, SUSANNE; STOECK, MATTHIAS; OLIVEIRA, MARCOS; NOBREGA, RAFAEL; ORMANNS, JENNY; KLOAS, WERNER; FERON, ROMAIN; KLOPP, CHRISTOPHE; PARRINELLO, HUGUES; JOURNOT, LAURENT; HE, SHUNPING; POSTLETHWAIT, JOHN; MEYER, AXEL; GUIGUEN, YANN; SCHARTL, MANFRED. The genome of the arapaima (Arapaima gigas) provides insights into gigantism, fast growth and chromosomal sex determination. SCIENTIFIC REPORTS, v. 9, MAR 28 2019. Web of Science Citations: 0.
BRANCO, GIOVANA SOUZA; MELO, ALINE GOMES; RICCI, JULIANA M. B.; DIGMAYER, MELANIE; DE JESUS, LAZARO W. O.; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males. General and Comparative Endocrinology, v. 273, n. SI, p. 209-217, MAR 1 2019. Web of Science Citations: 3.
DE SIQUEIRA-SILVA, DIOGENES HENRIQUE; RODRIGUES, MAIRA DA SILVA; NOBREGA, RAFAEL HENRIQUE. Testis structure, spermatogonial niche and Sertoli cell efficiency in Neotropical fish. General and Comparative Endocrinology, v. 273, n. SI, p. 218-226, MAR 1 2019. Web of Science Citations: 0.
LACERDA, S. M. S. N.; MARTINEZ, E. R. M.; MURA, I. L. D. D.; DORETTO, L. B.; COSTA, G. M. J.; SILVA, M. A.; DIGMAYER, M.; NOBREGA, R. H.; FRANCA, L. R. Duration of spermatogenesis and identification of spermatogonial stem cell markers in a Neotropical catfish, Jundia (Rhamdia quelen). General and Comparative Endocrinology, v. 273, n. SI, p. 249-259, MAR 1 2019. Web of Science Citations: 0.
TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, n. SI, p. 230-236, SEP 1 2018. Web of Science Citations: 7.
RICCI, JULIANA M. B.; MARTINEZ, EMANUEL R. M.; BUTZGE, ARNO J.; DORETTO, LUCAS B.; OLIVEIRA, MARCOS A.; BOMBARDELLI, ROBIE ALLAN; BOGERD, JAN; NOBREGA, RAFAEL H. Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development. Gene, v. 654, p. 116-126, MAY 15 2018. Web of Science Citations: 3.
ASSIS, LUIZ HENRIQUE DE CASTRO; DE NOBREGA, RAFAEL HENRIQUE; ESTHER GOMEZ-GONZALEZ, NURIA; BOGERD, JAN; SCHULZ, RUDIGER WINFRIED. Estrogen-induced inhibition of spermatogenesis in zebrafish is largely reversed by androgen. JOURNAL OF MOLECULAR ENDOCRINOLOGY, v. 60, n. 4, p. 273-284, MAY 2018. Web of Science Citations: 8.
MORAIS, R. D. V. S.; CRESPO, D.; NOBREGA, R. H.; LEMOS, M. S.; VAN DE KANT, H. J. G.; DE FRANCA, L. R.; MALE, R.; BOGERD, J.; SCHULZ, R. W. Antagonistic regulation of spermatogonial differentiation in zebrafish (Danio rerio) by Igf3 and Amh. Molecular and Cellular Endocrinology, v. 454, n. C, p. 112-124, OCT 15 2017. Web of Science Citations: 11.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.