Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species

• Grant number: 14/07620-7
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: August 01, 2015
• End date: July 31, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Rafael Henrique Nóbrega
• Grantee: Rafael Henrique Nóbrega
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant(s): 21/06742-5 - New insights on gonadal signals (growth factors and lncRNAs) that regulate gonadal maturation in fish: basic knowledge and development of biotechnological strategies to delay puberty, AP.JP2
• Associated scholarship(s): 18/16595-7 - Kisspeptins and its role in reproduction and gonadal development in teleost fish., BP.DR; 18/10265-5 - Endocrine and molecular mechanisms involved in the sexual differentiation of Medaka (Oryzias latipes) under conditions of thermal stress, BP.MS; 18/17111-3 - Identification of spermatogonial stem cell in zebrafish, Danio rerio, BP.IC; + associated scholarships 17/15793-7 - Effects of hypothyroidism on testicular function of Danio rerio and relation between thyroid hormones and follicle stimulating hormone (Fsh), BP.MS; 16/12101-4 - Selection, Culture and Transcriptome of spermatogonial stem cells of zebrafish, Danio rerio: a functional and genomics approach, BP.MS; 15/16796-4 - Molecular characterization of jundiá Rhamdia quelen spermatogonial stem cells, BP.IC - associated scholarships

Abstract

Spermatogonial Stem Cells (SSCs) are the only stem cells in the body capable to transmit the genetic information from species along generations. This feature is due to the potential of these cells to either self-renew or differentiate to give rise to millions of spermatozoa. Recent studies using SSCs have generated promising methods in the aquaculture, such as the SSC transplantation. This technique consists in the isolation of donor SSCs and their transplantation into a recipient testis, where these cells can develop and produce sperm with donor genetic characteristics. In the current project, we propose a SSC bank which in association with transplantation techniques constitutes a powerful tool to preserve endangered and valuable species. Therefore, the aim of this project is to characterize the molecular markers of SSCs using zebrafish (Danio rerio) as a laboratory model; and to apply this knowledge for a native and high economic interest species, such as jundiá (Rhamdia quelen). Then, SSCs from jundiá will be selected based on these molecular markers, and submitted to cryopreservation techniques afterwards. To test the viability of the cryopreserved cells, SSCs will be thawed and transplanted into recipient testes. In addition, we aimed to generate vasa::gfp jundiás in order to improve the the visualization of the donor-derived spermatogenesis in the recipient testes. This project will provide the basis to create a SSC bank for several teleost species in order to preserve the genotype of endangered and high economic interest species. (AU)

Scientific publications

(22)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAIS, R. D. V. S.; CRESPO, D.; NOBREGA, R. H.; LEMOS, M. S.; VAN DE KANT, H. J. G.; DE FRANCA, L. R.; MALE, R.; BOGERD, J.; SCHULZ, R. W.. Antagonistic regulation of spermatogonial differentiation in zebrafish (Danio rerio) by Igf3 and Amh. Molecular and Cellular Endocrinology, v. 454, n. C, p. 112-124, OCT 15 2017. (12/00423-6, 14/07620-7)

LACERDA, S. M. S. N.; MARTINEZ, E. R. M.; MURA, I. L. D. D.; DORETTO, L. B.; COSTA, G. M. J.; SILVA, M. A.; DIGMAYER, M.; NOBREGA, R. H.; FRANCA, L. R.. Duration of spermatogenesis and identification of spermatogonial stem cell markers in a Neotropical catfish, Jundia (Rhamdia quelen). General and Comparative Endocrinology, v. 273, n. SI, p. 249-259, MAR 1 2019. (14/07620-7, 12/00423-6, 13/22778-3)

RODRIGUES, MAIRA S.; FALLAH, HAMIDEH P.; ZANARDINI, MAYA; MALAFAIA, GUILHERME; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Interaction between thyroid hormones and gonadotropin inhibitory hormone in ex vivo culture of zebrafish testis: An approach to study multifactorial control of spermatogenesis. Molecular and Cellular Endocrinology, v. 532, JUL 15 2021. (17/15793-7, 18/15319-6, 14/07620-7)

XIE, XUAN; NOBREGA, RAFAEL; PSENICKA, MARTIN. Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems. BIOMOLECULES, v. 10, n. 4, APR 2020. (14/07620-7)

TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, p. 7-pg., 2018-09-01. (17/09218-0, 14/07620-7, 14/16280-5, 17/15793-7)

DE SIQUEIRA-SILVA, DIOGENES HENRIQUE; RODRIGUES, MAIRA DA SILVA; NOBREGA, RAFAEL HENRIQUE. Testis structure, spermatogonial niche and Sertoli cell efficiency in Neotropical fish. General and Comparative Endocrinology, v. 273, p. 9-pg., 2019-03-01. (14/07620-7, 17/15793-7)

RODRIGUES, MAIRA S.; TOVO-NETO, ALDO; ROSA, IVANA F.; DORETTO, LUCAS B.; FALLAH, HAMIDEH P.; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and-Gonadal Axes in Zebrafish. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 10, p. 16-pg., 2022-05-12. (17/15793-7, 14/07620-7, 18/15319-6, 20/03569-8)

TOVO-NETO, ALDO; MARTINEZ, EMANUEL R. M.; MELO, ALINE G.; DORETTO, LUCAS B.; BUTZGE, ARNO J.; RODRIGUES, MAIRA S.; NAKAJIMA, RAFAEL T.; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Cortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants. BIOMOLECULES, v. 10, n. 3, MAR 2020. (14/07620-7, 14/16280-5, 13/22778-3, 17/15793-7, 16/12101-4, 15/12104-0, 17/09218-0)

RICCI, JULIANA M. B.; MARTINEZ, EMANUEL R. M.; BUTZGE, ARNO J.; DORETTO, LUCAS B.; OLIVEIRA, MARCOS A.; BOMBARDELLI, ROBIE ALLAN; BOGERD, JAN; NOBREGA, RAFAEL H.. Characterization of &ITvasa&IT homolog in a neotropical catfish, Jundia (&ITRhamdia quelen&IT): Molecular cloning and expression analysis during embryonic and larval development. Gene, v. 654, p. 116-126, MAY 15 2018. (12/00423-6, 14/07620-7)

DU, KANG; WUERTZ, SVEN; ADOLFI, MATEUS; KNEITZ, SUSANNE; STOECK, MATTHIAS; OLIVEIRA, MARCOS; NOBREGA, RAFAEL; ORMANNS, JENNY; KLOAS, WERNER; FERON, ROMAIN; et al. The genome of the arapaima (Arapaima gigas) provides insights into gigantism, fast growth and chromosomal sex determination. SCIENTIFIC REPORTS, v. 9, MAR 28 2019. (14/25313-4, 14/50602-0, 15/15631-1, 14/07620-7)

MORAIS, R. D. V. S.; CRESPO, D.; NOBREGA, R. H.; LEMOS, M. S.; VAN DE KANT, H. J. G.; DE FRANCA, L. R.; MALE, R.; BOGERD, J.; SCHULZ, R. W.. Antagonistic regulation of spermatogonial differentiation in zebrafish (Danio rerio) by Igf3 and Amh. Molecular and Cellular Endocrinology, v. 454, n. C, p. 13-pg., 2017-10-15. (14/07620-7, 12/00423-6)

PADILLA, LUISA F. ARIAS; CASTANEDA-CORTES, DIANA C.; ROSA, IVANA F.; ACOSTA, OMAR D. MORENO; HATTORI, RICARDO S.; NOBREGA, RAFAEL H.; FERNANDINO, I, JUAN. Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka. eLIFE, v. 10, MAR 1 2021. (18/10265-5, 14/07620-7)

BRANCO, GIOVANA SOUZA; MELO, ALINE GOMES; RICCI, JULIANA M. B.; DIGMAYER, MELANIE; DE JESUS, LAZARO W. O.; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males. General and Comparative Endocrinology, v. 273, n. SI, p. 209-217, MAR 1 2019. (14/07620-7, 15/12104-0, 13/20450-0)

DE SIQUEIRA-SILVA, DIOGENES HENRIQUE; RODRIGUES, MAIRA DA SILVA; NOBREGA, RAFAEL HENRIQUE. Testis structure, spermatogonial niche and Sertoli cell efficiency in Neotropical fish. General and Comparative Endocrinology, v. 273, n. SI, p. 218-226, MAR 1 2019. (14/07620-7, 17/15793-7)

TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, n. SI, p. 230-236, SEP 1 2018. (14/16280-5, 14/07620-7, 17/09218-0, 17/15793-7)

DORETTO, LUCAS B.; BUTZGE, ARNO J.; NAKAJIMA, RAFAEL T.; MARTINEZ, EMANUEL R. M.; DE SOUZA, BEATRIZ MARQUES; RODRIGUES, MAIRA DA SILVA; ROSA, IVANA F.; RICCI, JULIANA M. B.; TOVO-NETO, ALDO; COSTA, DANIEL F.; et al. Gdnf Acts as a Germ Cell-Derived Growth Factor and Regulates the Zebrafish Germ Stem Cell Niche in Autocrine- and Paracrine-Dependent Manners. CELLS, v. 11, n. 8, p. 27-pg., 2022-04-01. (17/08274-3, 14/07620-7, 16/12101-4, 20/03569-8)

MARTINEZ-BENGOCHEA, A.; DORETTO, L.; ROSA, I. F.; OLIVEIRA, M. A.; SILVA, C.; SILVA, D. M. Z. A.; SANTOS, G. R.; SANTOS, J. S. F.; AVELAR, M. M.; SILVA, V, L.; et al. Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 248, OCT-NOV 2020. (14/07620-7, 16/12101-4, 18/10265-5, 14/25313-4)

LACERDA, S. M. S. N.; MARTINEZ, E. R. M.; MURA, I. L. D. D.; DORETTO, L. B.; COSTA, G. M. J.; SILVA, M. A.; DIGMAYER, M.; NOBREGA, R. H.; FRANCA, L. R.. Duration of spermatogenesis and identification of spermatogonial stem cell markers in a Neotropical catfish, Jundia (Rhamdia quelen). General and Comparative Endocrinology, v. 273, p. 11-pg., 2019-03-01. (13/22778-3, 14/07620-7, 12/00423-6)

FALLAH, HAMIDEH P.; TOVO-NETO, ALDO; YEUNG, EDWARD C.; NOBREGA, RAFAEL H.; HABIBI, HAMID R.. Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone. Molecular and Cellular Endocrinology, v. 492, JUL 15 2019. (14/07620-7, 17/09218-0, 14/16280-5)

ASSIS, LUIZ HENRIQUE DE CASTRO; DE NOBREGA, RAFAEL HENRIQUE; ESTHER GOMEZ-GONZALEZ, NURIA; BOGERD, JAN; SCHULZ, RUDIGER WINFRIED. Estrogen-induced inhibition of spermatogenesis in zebrafish is largely reversed by androgen. JOURNAL OF MOLECULAR ENDOCRINOLOGY, v. 60, n. 4, p. 273-284, MAY 2018. (14/07620-7)

ANGEL ANDREAS ARIAS VIGOYA; DANIEL FERNANDES DA COSTA; MARCOS ANTÔNIO DE OLIVEIRA; ARNO JULIANO BUTZGE; IVANA FELIPE ROSA; LUCAS BENITES DORETTO; EMANUEL RICARDO MONTEIRO MARTINEZ; MELANIE DIGMAYER; RAFAEL HENRIQUE NÓBREGA. Time-efficient germ cell transplantation from goldfish (Carassius auratus) into adult common carp (Cyprinus carpio). Animal Reproduction, v. 21, n. 1, 2024-02-12. (14/07620-7, 12/00423-6, 21/06742-5)

BRANCO, GIOVANA SOUZA; MELO, ALINE GOMES; RICCI, JULIANA M. B.; DIGMAYER, MELANIE; DE JESUS, LAZARO W. O.; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males. General and Comparative Endocrinology, v. 273, p. 9-pg., 2019-03-01. (14/07620-7, 15/12104-0, 13/20450-0)