MHC class I chain-related gene A polymorphisms and linkage disequilibrium with HLAB and HLA-C alleles in ocular toxoplasmosis

• Grant number: 15/13723-6
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: September 01, 2015
• End date: February 29, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Cinara de Cássia Brandão
• Grantee: Cinara de Cássia Brandão
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

This study investigated whether polymorphisms of the MICA gene are associated withthe development of eye lesions related to infection by Toxoplasma gondii in a group ofimmunocompetent patients from southeastern Brazil. The study enrolled 297 patientswith serological diagnosis of toxoplasmosis, classified into two distinct groups afterconducting fundoscopic exams according to the presence of ocular scars/lesions (n =148) or absence of ocular scars/lesions (n = 149) due to toxoplasmosis. The group ofpatients with scars/lesions was further subdivided into two groups according to the typeof the ocular manifestation observed: primary (n = 120) or recurrent manifestations (n =28). Genotyping of the MICA and HLA alleles was performed by the polymerase chainreaction-sequence specific oligonucleotide technique (PCR-SSO; One Lambda®) andthe MICA-129 polymorphism (rs1051792) was identified by nested polymerase chainreaction (PCR-RFLP). Significant associations involving MICA polymorphisms were notfound. Although the MICA*002~HLA-B*35 haplotype was associated with increasedrisk of developing ocular toxoplasmosis (p-value = 0.04; OR = 2.20; 95% CI = 1.05-4.60), and the MICA*008~HLA-C*07 haplotype was associated with protection againstthe development of manifestations of ocular toxoplasmosis (p-value = 0.009; OR: 0.44;95% CI: 0.22-0.76), these associations lost statistical significance after adjusting formultiple comparisons. MICA polymorphisms do not appear to influence thedevelopment of ocular lesions in patients diagnosed with toxoplasmosis in this studypopulation. For a better understanding of the influence of MICA~HLA haplotypes asrisk factors for ocular toxoplasmosis we suggest that further studies should beconducted, in particular involving families. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AYO, CHRISTIANE MARIA; DA SILVEIRA CAMARGO, ANA VITORIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; BARBOSA, AMANDA PIRES; BRANDAO DE MATTOS, CINARA DE CASSIA; DE MATTOS, LUIZ CARLOS. MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis. PLoS One, v. 10, n. 12, DEC 16 2015. (13/15879-8, 13/25650-8, 13/06580-9, 13/10050-5, 09/17540-2, 15/13723-6)