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Evaluation of reciprocating preparation, root canal filling, and the physicochemical and biological properties of filling and repair materials

Grant number: 15/03437-6
Support type:Regular Research Grants
Duration: September 01, 2015 - August 31, 2017
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Mario Tanomaru Filho
Grantee:Mario Tanomaru Filho
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Assoc. researchers:Juliane Maria Guerreiro Tanomaru ; Marco Antonio Hungaro Duarte


Constant evolution is observed in the development of techniques and kinematics of root canal preparation using Nickel-Titanium instruments, the root canal filling and repair materials and obturation techniques. The proposed studies aim to evaluate the preparation of oval root canals using the rotating or reciprocating kinematic, filling techniques, and physicochemical and biological properties of calcium silicate materials.Subproject 1 - Root canals of human mandibular extracted incisors with single oval root canal will be prepared by Nickel-Titanium instruments in rotating or reciprocating kinematic. The samples will be scanned with MicroCT SkyScan 1176 (Bruker-microCT, Belgium) and evaluated after preparation and after filling with different materials and techniques. Specimens will be also evaluated using histological analysis, and SEM .Subproject 2 - This study aims to develop cements based on tricalcium silicate or tricalcium aluminate associated with alternative radiopacifying agents. They will be compared to MTA Angelus, Biodentine and experimental cement Quick-Set. The following tests will be performed: setting time, flow, radiopacity, fluid uptake, pH, calcium and resistance to compression according to standard methods ISO 6876 and ISO 4049. Furthermore, cytotoxicity, and bioactivity will be examined in cell culture, chemical characterization using Energy Dispersive x-ray, scanning electron microscopy (SEM-EDX), x-ray Diffraction (XDR), infrared spectroscopy (FTIR). The antimicrobial activity will be assessed by direct contact test.Subproject 3 - The objective of this study is to evaluate the solubility, dimensional change, filling capacity, flow and porosity of root-end filling materials; and solubility, dimensional changes and porosity of sealers by using conventional tests, compared to proposed new methodologies using Micro-CT.Subproject 4 - The objective of the study is to evaluate the biological behavior and mineralized tissue formation of a tricalcium silicate experimental material with 20% zirconium oxide and MTA Plus. Human osteoblastic cells SAOS-2 (ATCC HTB-85) will be used for analysis of cellular cytotoxicity and bioactivity. Furthermore, the tissue response caused by the different materials will be evaluated in the subcutaneous tissue of rats. (AU)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, E. M.; CORNELIO, A. L. G.; GODOI, P. H.; DA COSTA, I, P.; ROSSA-JUNIOR, C.; FARIA, G.; GUERREIRO TANOMARU, J. M.; TANOMARU-FILHO, M. Heparin is biocompatible and can induce differentiation of human dental pulp cells. International Endodontic Journal, v. 52, n. 6, p. 829-837, JUN 2019. Web of Science Citations: 0.
ESPIR, CAMILA GALLETTI; NASCIMENTO-MENDES, CAMILA ALMEIDA; GUERREIRO-TANOMARU, JULIANE MARIA; CAVENAGO, BRUNO CAVALINI; HUNGARO DUARTE, MARCO ANTONIO; TANOMARU-FILHO, MARIO. Shaping ability of rotary or reciprocating systems for oval root canal preparation: a micro-computed tomography study. CLINICAL ORAL INVESTIGATIONS, v. 22, n. 9, p. 3189-3194, DEC 2018. Web of Science Citations: 2.
ESPIR, C. G.; NASCIMENTO-MENDES, C. A.; GUERREIRO-TANOMARU, J. M.; FREIRE, L. G.; GAVINI, G.; TANOMARU-FILHO, M. Counterclockwise or clockwise reciprocating motion for oval root canal preparation: a micro-CT analysis. International Endodontic Journal, v. 51, n. 5, p. 541-548, MAY 2018. Web of Science Citations: 6.
ESTEVES TORRES, FERNANDA FERRARI; GUERREIRO-TANOMARU, JULIANE MARIA; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE MORAIMA; TANOMARU-FILHO, MARIO. Solubility, porosity and fluid uptake of calcium silicate-based cements. Journal of Applied Oral Science, v. 26, p. -, 2018. Web of Science Citations: 2.
TANOMARU-FILHO, M.; ANDRADE, A. S.; RODRIGUES, E. M.; VIOLA, K. S.; FARIA, G.; CAMILLERI, J.; GUERREIRO-TANOMARU, J. M. Biocompatibility and mineralized nodule formation of Neo MTA Plus and an experimental tricalcium silicate cement containing tantalum oxide. International Endodontic Journal, v. 50, n. 2, SI, p. E31-E39, DEC 2017. Web of Science Citations: 14.
TANOMARU-FILHO, MARIO; ESTEVES TORRES, FERNANDA FERRARI; CHAVEZ-ANDRADE, GISSELLE MORAIMA; DE ALMEIDA, MADELISE; NAVARRO, LUCIANA GUILHERME; STEIER, LIVIU; GUERREIRO-TANOMARU, JULIANE MARIA. Physicochemical Properties and Volumetric Change of Silicone/Bioactive Glass and Calcium Silicate-based Endodontic Sealers. JOURNAL OF ENDODONTICS, v. 43, n. 12, p. 2097-2101, DEC 2017. Web of Science Citations: 7.
ESTEVES TORRES, FERNANDA FERRARI; BOSSO-MARTELO, ROBERTA; ESPIR, CAMILA GALLETTI; CIRELLI, JONI AUGUSTO; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests. Journal of Applied Oral Science, v. 25, n. 4, p. 374-380, JUL-AUG 2017. Web of Science Citations: 8.
TANOMARU-FILHO, MARIO; ESTEVES TORRES, FERNANDA FERRARI; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE MORAIMA; BONETTI-FILHO, IDOMEO; GUERREIRO-TANOMARU, JULIANE MARIA. A Novel Model for Evaluating the Flow of Endodontic Materials Using Micro-computed Tomography. JOURNAL OF ENDODONTICS, v. 43, n. 5, p. 796-800, MAY 2017. Web of Science Citations: 4.

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