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Multi-use instrument approved and awarded in the project 2013/50228-8, name of instrument: ultra-performance liquid chromatograph coupled with a high resolution mass spectrometer

Grant number: 15/01017-0
Support type:Multi-user Equipment Program
Duration: October 01, 2015 - September 30, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Roberto Gomes de Souza Berlinck
Grantee:Roberto Gomes de Souza Berlinck
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:13/50228-8 - Biodiversity components, and its metabolic characters, of Brazilian Islands, AP.BTA.TEM
EMU web page: Página do Equipamento Multiusuário não informada
Use scheduling: E-mail de agendamento não informado

Abstract

The chromatograph UPLC-MS / MS with accessories (including, among other items, detection system for tandem MS QTof, vacuum pump, LockSpray source with electrospray source, Lenovo digital monitor, ultra-performance chromatograph Acquity, arrangement detector diodes, MassLynx software, nitrogen generator, samples of chromatographic columns) should be used for the following purposes: a) analysis of the presence of antimicrobial peptides in coral samples Mussismilia spp. as well as microbial strains isolated from the same coral that these peptides should be uniquely identified by high resolution fragmentation analysis MS / MS; b) analyzing the presence of bryostatins and other complex polyketides present in Bugula spp., as well as microorganisms that possibly may be isolated and cultured from the bryozoan. These policetídios should also be clearly identified by high resolution fragmentation analysis MS / MS; c) analysis of the presence of tetrodotoxinas and derivatives in Tetraodon spp. (puffer fish) and micro-organisms associated to be isolated and identified from these fish. Toxins should also be identified by high resolution fragmentation analysis MS / MS; d) in dereplication experiments for unambiguous identification of known substances in micro-organisms and marine invertebrates extracts, seeking to identify those known so that we can prioritize the most isolating novel bioactive substances; e) identification of peptides Cnidaria, to be isolated by Prof. team André Tempone (Instituto Adolfo Lutz, SP). The main advantages of USE UPLC-UV-MS / MS are: ultra performance chromatographic separation, allowing the analysis with extreme accuracy the mass and UV spectrum of minority substances (often the most interested), use of different probes for ionization material crude (less clean) and equipment better prepared (as semi-purified samples) to investigate in detail the fragmentation tandem (MS / MS) in order to unequivocally identify the substances present in the samples, constructing a database for holding chemical profile analyzes, dereplication and identification of substances belonging to specific classes (bryostatins, tetrodotoxinas, polypeptides), exact mass measurements analysis to establish the atomic composition of unknown substances, possibility to use different ionization sources (ESI, APCI, CSE, APPI nanoFlow ESI) in a single apparatus for performing analyzes for different purposes aiming at the detection of the metabolic substances of different classes. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
KURODA, YUSUKE; NICACIO, KAREN J.; DA SILVA-, JR., ILDEFONSO ALVES; LEGER, PAUL R.; CHANG, STANLEY; GUBIANI, JULIANA R.; DEFLON, VICTOR M.; NAGASHIMA, NOZOMU; RODE, ALEXANDER; BLACKFORD, KATHERINE; FERREIRA, ANTONIO G.; SETTE, LARA D.; WILLIAMS, DAVID E.; ANDERSEN, RAYMOND J.; JANCAR, SONIA; BERLINCK, ROBERTO G. S.; SARPONG, RICHMOND. Isolation, synthesis and bioactivity studies of phomactin terpenoids. NATURE CHEMISTRY, v. 10, n. 9, p. 938-945, SEP 2018. Web of Science Citations: 4.
RAMIREZ, MARIA CAMILA A.; WILLIAMS, DAVID E.; GUBIANI, JULIANA R.; PARRA, LIZBETH L. L.; SANTOS, MARIO F. C.; FERREIRA, DAIANE D.; MESQUITA, JULIANA T.; TEMPONE, ANDRE G.; FERREIRA, ANTONIO G.; PADULA, VINICIUS; HAJDU, EDUARDO; ANDERSEN, RAYMOND J.; BERLINCK, ROBERTO G. S. Rearranged Terpenoids from the Marine Sponge Darwinella cf. oxeata and Its Predator, the Nudibranch Felimida grahami. Journal of Natural Products, v. 80, n. 3, SI, p. 720-725, MAR 2017. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.