Hybrid device PVA/collagen/hyaluronic acid cultured with mesenchymal stem cells for dermal regeneration

• Grant number: 15/06883-7
• Support type: Regular Research Grants
• Duration: March 01, 2016 - May 31, 2018
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal researcher: Eliana Aparecida de Rezende Duek
• Grantee: Eliana Aparecida de Rezende Duek
• Home Institution: Faculdade de Ciências Médicas e da Saúde (FCMS). Pontifícia Universidade Católica de São Paulo (PUC-SP). Sorocaba , SP, Brazil

Abstract

Currently there are several dermal substitutes, but these are also associated with a range of problems such as reduced vascularization, formation of excessive fibrosis, lack of flexibility, inability to maintain hydration of the wound, among others. This study proposes to develop a skin equivalent that meets any of the above requirements. The device bilayer developed is composed of polyvinyl alcohol (PVA) - PVA membrane as epidermal analogue, in order to hydration and the lesion exudate absorption, and dermic analogue is used as a collagen/hyaluronic acid scaffold mimicking the extracellular matrix. The collagen/HA scaffold will be cultivated with mesenchymal stem cells derived from human adipose tissue (ASCs). The ASCs will be used due to its anti-fibrotic and angiogenic potential. It is believed that this anti-fibrotic potential is due to ability immunomodulatory of ASCs, since the formation of fibrosis is associated with persistent inflammatory processes at the site of injury. One of the main immune cells involved in the wound healing process is the macrophage. Several studies have demonstrated the polarization of macrophages induced by ASCs for an anti-inflammatory phenotype. Therefore this study besides of development of bilaminar device and its characterizations will be available the maintenance of the immunomodulatory potential of ASCs on macrophages after growing in the collagen/ HA scaffolds. Immunomodulatory ability of ASCs with his anti-fibrotic potential will be realized growing these cells and macrophages in the collagen/HA scaffolds and co-cultured with fibroblasts in TRANSWELL® system. This co-culture with fibroblasts will allow analyzing the paracrine effects of immunomodulation of ASCs. Preliminary results demonstrated that it was possible to obtain the bilayer device PVA / collagen / HA and it was isolated and expanded adipose tissue cells, which were confirmed as mesenchymal stem cells by flow cytometry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KOMATSU, DANIEL; HAUSEN, MOEMA A.; ERI, RICARDO YUGI; LEAL, VINICIUS; PEDRINI, FLAVIA; YAKSIC, CAMILO; ALVES, THAIS F. R.; CHAUD, V, MARCO; FANELLI, CAMILLA; NORONHA, IRENE; et al. Alternative Cutaneous Substitutes Based on Poly(L-co-D,L-lactic acid-co-trimethylene carbonate) with Schinus terebinthifolius Raddi Extract Designed for Skin Healing. ACS OMEGA, v. 4, n. 19, p. 18317-18326, NOV 5 2019. (15/06883-7)

MENDES JUNIOR, DARIO; HAUSEN, MOEMA A.; ASAMI, JESSICA; HIGA, AKEMI M.; LEITE, FABIO L.; MAMBRINI, GIOVANNI P.; ROSSI, ANDRE L.; KOMATSU, DANIEL; DUEK, ELIANA A. DE REZENDE. A New Dermal Substitute Containing Polyvinyl Alcohol with Silver Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches. ANTIBIOTICS-BASEL, v. 10, n. 6, JUN 2021. (15/06883-7)

KOMATSU, DANIEL; MISTURA, DANIEL V.; MOTTA, ADRIANA; DOMINGUES, JULIANA A.; HAUSEN, MOEMA A.; DUEK, ELIANA. Development of a membrane of poly (L-co-D,L lactic acid-co-trimethylene carbonate) with aloe vera: An alternative biomaterial designed to improve skin healing. JOURNAL OF BIOMATERIALS APPLICATIONS, v. 32, n. 3, p. 311-320, SEP 2017. (14/11858-9, 15/06883-7)