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Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis

Grant number: 14/50981-0
Support type:Research Projects - Thematic Grants
Duration: November 01, 2015 - November 30, 2020
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Ana Lucia Tabet Oller Do Nascimento
Grantee:Ana Lucia Tabet Oller Do Nascimento
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated scholarship(s):18/06201-1 - Identification of virulence factors of Leptospira interrogans through comparative proteomics analysis, BP.DR
18/08131-0 - Heterologous expression of the gene LIC13259 in the saprophyte Leptospira biflexa and their acquisition capacity to evade the human immune system, BP.DR
18/21959-8 - Evaluation of the functional role of HtrA-like proteins present in pathogenic strains by creating mutants of Leptospira biflexa and Leptospira interrogans, BP.DD
+ associated scholarships 18/09652-4 - Evaluation of the interaction of Leptospira interrogans proteins with host components by constructing recombinant Leptospira biflexa, BP.DR
17/26223-7 - Evaluation of functional role of Leptospira interrogans surface proteins as endothelial and epithelial cells receptors of the host, BP.DD
17/25167-6 - Structural characterization of surface proteins of Leptospira interrogans and thermodynamics of the interaction with host-components, BP.PD
17/06731-8 - Development of new strategies for genetic manipulation in Leptospira spp.: antisense RNA and CRISPR/Cas9, BP.PD
17/01102-2 - Biochemical and immunological evaluation of hypothetical surface proteins of Leptospira interrogans, BP.DD
17/01411-5 - Cloning, expression and characterization of HtrA proteins of Leptospira interrogans, BP.MS
16/04295-3 - Interaction of two Leptospira interrogans proteins to plasma and extracellular matrix components of the host, BP.MS
16/11541-0 - "evaluation of immunoprotective potential of CD4+ T-cell epitopes identified in proteins of Leptospira interrogans", BP.PD
16/01384-5 - Evaluation of the functional role of two Leptospira interrogans proteins in the adhesion process of the pathogen to the host, BP.MS - associated scholarships

Abstract

Leptospirosis is a global wide spread zoonosis caused by pathogenic spirochetes of the genus Leptospira. There is no effective vaccine. The Identification of surface proteins among pathogenic strains is the research focus to elucidated mechanisms involved (AU)

Scientific publications (20)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PASSALIA, FELIPE JOSE; CARVALHO, ENEAS; HEINEMANN, MARCOS BRYAN; VIEIRA, MONICA LARUCCI; NASCIMENTO, ANA LUCIA T. O. The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components. MICROBIOLOGICAL RESEARCH, v. 235, MAY 2020. Web of Science Citations: 0.
VIEIRA, MONICA L.; HERWALD, HEIKO; NASCIMENTO, ANA LUCIA T. O. The interplay between host haemostatic systems and Leptospira spp. infections. CRITICAL REVIEWS IN MICROBIOLOGY, v. 46, n. 2 MAR 2020. Web of Science Citations: 0.
TEIXEIRA, ALINE F.; FERNANDES, V, LUIS G.; CAVENAGUE, MARIA F.; TAKAHASHI, MARIA B.; SANTOS, JADEMILSON C.; PASSALIA, FELIPE J.; DAROZ, BRENDA B.; KOCHI, LEANDRO T.; VIEIRA, MONICA L.; NASCIMENTO, ANA L. T. O. Adjuvanted leptospiral vaccines: Challenges and future development of new leptospirosis vaccines. Vaccine, v. 37, n. 30, p. 3961-3973, JUL 9 2019. Web of Science Citations: 1.
VIEIRA, MONICA L.; PERSSON, SANDRA; LOPES-FERREIRA, MONICA; ROMERO, ELIETE C.; KIRCHGATTER, KARIN; NASCIMENTO, ANA LUCIA T. O.; HERWALD, HEIKO. Heparin-Binding Protein Release Is Strongly Induced by Leptospira Species and Is a Candidate for an Early Diagnostic Marker of Human Leptospirosis. Journal of Infectious Diseases, v. 219, n. 6, p. 996-1006, MAR 15 2019. Web of Science Citations: 0.
CAVENAGUE, MARIA F.; TEIXEIRA, ALINE F.; FILHO, ANTONIO S.; SOUZA, GISELE O.; VASCONCELLOS, SILVIO A.; HEINEMANN, MARCOS B.; NASCIMENTO, ANA L. T. O. Characterization of a novel protein of Leptospira interrogans exhibiting plasminogen, vitronectin and complement binding properties. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, v. 309, n. 2, p. 116-129, MAR 2019. Web of Science Citations: 2.
FERNANDES, L. G. V.; GUAMAN, L. P.; VASCONCELLOS, S. A.; HEINEMANN, MARCOS B.; PICARDEAU, M.; NASCIMENTO, A. L. T. O. Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira. SCIENTIFIC REPORTS, v. 9, FEB 12 2019. Web of Science Citations: 0.
LOPES, ALEXANDRE P. Y.; AZEVEDO, BRUNA O. P.; EMIDIO, REBECA C.; DAMIANO, DEBORAH K.; NASCIMENTO, ANA L. T. O.; BARAZZONE, GIOVANA C. In Silico Analysis of Genetic VapC Profiles from the Toxin-Antitoxin Type II VapBC Modules among Pathogenic, Intermediate, and Non-Pathogenic Leptospira. MICROORGANISMS, v. 7, n. 2 FEB 2019. Web of Science Citations: 0.
KOCHI, LEANDRO T.; FERNANDES, V, LUIS G.; SOUZA, GISELE O.; VASCONCELLOS, SILVIO A.; HEINEMANN, MARCOS B.; ROMERO, ELIETE C.; KIRCHGATTER, KARIN; NASCIMENTO, ANA L. T. O. The interaction of two novel putative proteins of Leptospira interrogans with E-cadherin, plasminogen and complement components with potential role in bacterial infection. VIRULENCE, v. 10, n. 1, p. 734-753, JAN 1 2019. Web of Science Citations: 0.
NASCIMENTO FILHO, EDSON G.; VIEIRA, MONICA L.; TEIXEIR, ALINE F.; SANTOS, JADEMILSON C.; FERNANDES, LUIS G. V.; PASSALIA, FELIPE J.; DAROZ, BRENDA B.; ROSSINI, AMANDA; KOCHI, LEANDRO T.; CAVENAGUE, MARIA F.; PIMENTA, DANIEL C.; NASCIMENTO, ANA L. T. O. Proteomics as a tool to understand Leptospira physiology and virulence: Recent advances, challenges and clinical implications. JOURNAL OF PROTEOMICS, v. 180, n. SI, p. 80-87, MAY 30 2018. Web of Science Citations: 1.
SANTOS, JADSON V.; PEREIRA, PRISCILA R. M.; FERNANDES, LUIS G. V.; SIQUEIRA, GABRIELA HASE; DE SOUZA, GISELE O.; SOUZA FILHO, ANTONIO; VASCONCELLOS, SILVIO A.; HEINEMANN, MARCOS B.; CHAPOLA, ERICA G. B.; NASCIMENTO, ANA L. T. O. Binding of human plasminogen by the lipoprotein LipL46 of Leptospira interrogans. MOLECULAR AND CELLULAR PROBES, v. 37, p. 12-21, FEB 2018. Web of Science Citations: 3.
TEIXEIRA, ALINE F.; FERNANDES, LUIS G. V.; SOUZA FILHO, ANTONIO; SOUZA, GISELE O.; VASCONCELLOS, SILVIO A.; HEINEMANN, MARCOS B.; NASCIMENTO, ANA L. T. O. Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis. BIOMED RESEARCH INTERNATIONAL, 2018. Web of Science Citations: 2.
VIEIRA, MONICA L.; TEIXEIRA, ALINE F.; PIDDE, GISELLE; CHING, ANA T. C.; TAMBOURGI, DENISE V.; NASCIMENTO, ANA LUCIA T. O.; HERWALD, HEIKO. Leptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidase. VIRULENCE, v. 9, n. 1, p. 414-425, 2018. Web of Science Citations: 3.
SIQUEIRA, GABRIELA H.; DE SOUZA, GISELE O.; HEINEMANN, MARCOS B.; VASCONCELLOS, SILVIO A.; NASCIMENTO, ANA L. T. O. The role of Lsa23 to mediate the interaction of Leptospira interrogans with the terminal complement components pathway. Microbial Pathogenesis, v. 112, p. 182-189, NOV 2017. Web of Science Citations: 3.
PEREIRA, PRISCILA R. M.; FERNANDES, LUIS G. V.; DE SOUZA, GISELE O.; VASCONCELLOS, SILVIO A.; HEINEMANN, MARCOS B.; ROMERO, ELIETE C.; NASCIMENTO, ANA L. T. O. Multifunctional and Redundant Roles of Leptospira interrogans Proteins in Bacterial-Adhesion and fibrin clotting inhibition. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, v. 307, n. 6, p. 297-310, SEP 2017. Web of Science Citations: 6.
FERNANDES, LUIS G. V.; TEIXEIRA, ALINE F.; FILHO, ANTONIO F. S.; SOUZA, GISELE O.; VASCONCELLOS, SILVIO A.; HEINEMANN, MARCOS B.; ROMERO, ELIETE C.; NASCIMENTO, ANA L. T. O. Immune response and protective profile elicited by a multi-epitope chimeric protein derived from Leptospira interrogans. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 57, p. 61-69, APR 2017. Web of Science Citations: 9.
VIEIRA, MONICA L.; DE ANDRADE, SONIA A.; MORAIS, ZENAIDE M.; VASCONCELLOS, SILVIO A.; DAGLI, MARIA LUCIA Z.; NASCIMENTO, ANA LUCIA T. O. Leptospira Infection Interferes with the Prothrombinase Complex Assembly during Experimental Leptospirosis. FRONTIERS IN MICROBIOLOGY, v. 8, MAR 28 2017. Web of Science Citations: 0.
FIGUEREDO, JUPCIANA M.; SIQUEIRA, GABRIELA H.; DE SOUZA, GISELE O.; HEINEMANN, MARCOS B.; VASCONCELLOS, SILVIO A.; CHAPOLA, ERICA G. B.; NASCIMENTO, ANA L. T. O. Characterization of two new putative adhesins of Leptospira interrogans. MICROBIOLOGY-SGM, v. 163, n. 1, p. 37-51, JAN 2017. Web of Science Citations: 3.
COSATE, MARIA RAQUEL; SIQUEIRA, GABRIELA HASE; DE SOUZA, GISELE OLIVEIRA; VASCONCELLOS, SILVIO ARRUDA; NASCIMENTO, ANA LUCIA T. O. Mammalian cell entry (Mce) protein of Leptospira interrogans binds extracellular matrix components, plasminogen and 2 integrin. MICROBIOLOGY AND IMMUNOLOGY, v. 60, n. 9, p. 586-598, SEP 2016. Web of Science Citations: 3.
FERNANDES, LUIS G. V.; FILHO, ANTONIO F. S.; SOUZA, GISELE O.; VASCONCELLOS, SILVIO A.; ROMERO, ELIETE C.; NASCIMENTO, ANA L. T. O. Decrease in antithrombin III and prothrombin serum levels contribute to coagulation disorders during leptospirosis. MICROBIOLOGY-SGM, v. 162, n. 8, p. 1407-1421, AUG 2016. Web of Science Citations: 2.
SILVA, LUCAS P.; FERNANDES, LUIS G. V.; VIEIRA, MONICA L.; DE SOUZA, GISELE O.; HEINEMANN, MARCOS B.; VASCONCELLOS, SILVIO A.; ROMERO, ELIETE C.; NASCIMENTO, ANA L. T. O. Evaluation of two novel leptospiral proteins for their interaction with human host components. PATHOGENS AND DISEASE, v. 74, n. 5 JUL 2016. Web of Science Citations: 10.

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