Multi-User Equipment approved in grant 2013/11093-0: multi-mode microplate reader model synergy H1M- (monochromator based) Bio-Tek instruments, Inc. (em substituição a: Mithras LB 940 - Berthold)

• Grant number: 15/21179-4
• Support Opportunities: Multi-user Equipment Program
• Start date: February 01, 2016
• End date: January 31, 2023
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Nancy Amaral Reboucas
• Grantee: Nancy Amaral Reboucas
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/11093-0 - Molecular mechanisms involved in modulation of AT1R by ATRAP and associated proteins and effects on renal tubular ion transport, AP.R
• EMU web page: http://cefap.icb.usp.br/core-facilities/pluma/synergy-h1-leitor-de-microplacas-hibrido-multimodo/
• Type of equipment: Processos Biológicos - Caracterização - Leitores de placas
• Manufacturer: Bio-Tek
• Model: Synergy H1M

Abstract

In the research project, this equipment is essencial for the experiments designed to investigate the interactions between proteins in cells expressing fluorescent or luminescent protein tags (fusion proteins with luciferase) by FRET and BRET techniques, respectively. Due to its multi-function, this equipment will replace the Nonodrop spectrophotometer (asked in the thematic project 2013 / 23087-4, which therefore will no longer be acquired), the luminescence reading equipment, applied for Luciferase analysis used in experiments for promoter analysis with reporter genes, for biochemical analysis (such as creatinine and protein quantification), and ELISA, in which spectrometry is employed. In this way, multiple research projects will benefit from the multifunctional applications of the equipment, making it entirely appropriate for multiuser employment. (AU)