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The effects of spinal cord injury on the bone and bone callus microarchitecture, strength and metabolism in paraplegic rats. Are drugs, mechanical loading and ultrasound therapy effective at maintaining and improving skeletal health?

Grant number: 15/22126-1
Support Opportunities:Regular Research Grants
Duration: March 01, 2016 - August 31, 2018
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Ariane Zamarioli
Grantee:Ariane Zamarioli
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated researchers:José Batista Volpon


Spinal cord injury (SCI) is a condition that largely impairs bone turnover, increases fracture risk and is related to abnormal bone healing. Purpose: to assess the effects of SCI on the bone and bone callus quality in paraplegic rats, treated or not with pharmacological agents, passive standing and ultrasound therapy. Materials and methods: 96 rats will be divided into 6 groups: (1) Sham: control rats with bone fracture; (2) SCI: SCI rats with bone fracture; (3) SCI+ALE: SCI fractured rats treated with alendronate; (4) SCI+PTH: SCI fractured rats treated with PTH; (5) SCI+PS: SCI fractured rats treated with passive standing and; (6) SCI+US: SCI fractured rats treated with ultrasound. SCI rats will undergo a spinal surgery for complete spinal cord transection at the T10 level. 21 days after surgery rats will undergo a closed bone fracture at the mid-femur. Then, bone fragments will be stabilized with orthopedic implant. Bone healing will be followed by 14 days. Rats will receive subcutaneous PTH and alendronate 5d/wk. Passive standing and ultrasound will be administered 5d/wk for 20 minutes. Bones will be assessed by: optical microscopy; ¼CT; immunohistochemistry; qPCR; BMD; mechanical test and; serum bone marker and adipokines. These results will allow a better understanding of the mechanisms that lead to bone loss and changes in bone healing due to disuse. Furthermore, the effects of pharmacological agents, mechanical loading and ultrasound therapy at improving or maintaining bone quality and bone callus formation will be investigated. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUTEZLOFF, MARIANA M.; VOLPON, JOSE B.; XIMENEZ, JOAO P. B.; ASTOLPHO, KELLY; CORRELO, VITOR M.; REIS, RUI L.; SILVA, RAQUEL B.; ZAMARIOLI, ARIANE. Gene expression changes are associated with severe bone loss and deficient fracture callus formation in rats with complete spinal cord injury. SPINAL CORD, v. 58, n. 3, p. 365-376, . (15/22126-1)
ZAMARIOLI, ARIANE; BUTEZLOFF, MARIANA M.; XIMENEZ, JOAO P. B.; VOLPON, JOSE B.. Low-intensity pulsed ultrasound partially reversed the deleterious effects of a severe spinal cord injury-induced bone loss and osteoporotic fracture healing in paraplegic rats. SPINAL CORD, v. 61, n. 2, p. 9-pg., . (15/22126-1)

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