Improvement of the polysaccharide chitosan properties for its application in liposomes and giant vesicles

• Grant number: 15/23948-5
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Omar Mertins
• Grantee: Omar Mertins
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Sang Won Han

Abstract

With this project we intend to structure and establish a line of research whose main focus is the study and implementation of reactions to modify the polysaccharide chitosan by binding molecules with specific advantages aiming to increase the physical and chemical stability properties of lipid membranes that mimic the biological membrane. We also intend to study the application of this composite system, lipid/modified chitosan as an antioxidant product and gene transfer, as well to develop a modified chitosan gel with advantageous properties for its application. The highly interdisciplinary project will develop studies of interaction between the modified polymer and the membrane of lipids in spherical colloidal structures in the nanometer and micrometer range, respectively of liposomes and giant vesicles. Following, studies to increase the stability of the lipid membrane coated with modified chitosans will be performed using specific molecules that promote the degradation of lipids, such as with the use of photo active molecules that produce oxygen radicals under light irradiation which react with the lipids unsaturations. Next, in vitro studies will be performed with the composite system and the modified chitosan gel using specific cell lines. After, to liposomes coated with chitosan, plasmids will be incorporated aiming increasing the stability of these nucleic acids for efficient transfer to the cell nucleus with minimal toxicity. The project has a major focus in starting the scientific activities in the Institution where I have been recently hired, as well as training of students in undergraduate, master and PhD levels. (AU)

Scientific publications

(20)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATHEWS, PATRICK D.; PATTA, ANA C. M. FERNANDES; GONCALVES, JOAO V.; GAMA, GABRIELLA DOS SANTOS; GARCIA, IRENE TERESINHA SANTOS; MERTINS, OMAR. Targeted Drug Delivery and Treatment of Endoparasites with Biocompatible Particles of pH-Responsive Structure. Biomacromolecules, v. 19, n. 2, p. 499-510, FEB 2018. (16/13368-4, 17/16722-6, 15/23948-5)

MATHEWS, PATRICK D.; PATTA, ANA C. M. F.; GAMA, GABRIELLA S.; MERTINS, OMAR. Infestation by Ergasilus coatiarus (Copepoda: Ergasilidae) in two Amazonian cichlids with new host record from Peru: An ectoparasites natural control approach. COMPTES RENDUS BIOLOGIES, v. 341, n. 1, p. 16-19, JAN 2018. (16/25919-5, 15/23948-5)

MERTINS, OMAR; LOBO, SONJA ELLEN; MATHEWS, PATRICK D.; HAN, SANG WON. Interaction of pDNA with reverse phase chitosome. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 543, p. 76-82, APR 20 2018. (16/13368-4, 15/20206-8, 15/23948-5)

FERNANDES PATTA, ANA C. M.; MATHEWS, PATRICK D.; MADRID, RAFAEL R. M.; RIGONI, VERA L. S.; SILVA, EMERSON R.; MERTINS, OMAR. Polyionic complexes of chitosan-N-arginine with alginate as pH responsive and mucoadhesive particles for oral drug delivery applications. International Journal of Biological Macromolecules, v. 148, p. 550-564, APR 1 2020. (16/13368-4, 17/16722-6, 18/20482-3, 15/23948-5, 16/24409-3)

MATHEWS, PATRICK D.; MERTINS, OMAR; PEREIRA, JOSE O. L.; MAIA, ANTONIO A. M.; ADRIANO, EDSON A.. Morphology and 18S rDNA sequencing of Henneguya peruviensis n. sp (Cnidaria: Myxosporea), a parasite of the Amazonian ornamental fish Hyphessobrycon loretoensis from Peru: A myxosporean dispersal approach. Acta Tropica, v. 187, p. 207-213, NOV 2018. (13/14656-5, 15/23948-5, 13/21374-6)

RIVADENEYRA, NORMA L. S.; MERTINS, OMAR; CUADROS, RUBEN C.; MALTA, JOSE C. O.; DE MATOS, LORENA V.; MATHEWS, PATRICK D.. Histopathology associated with infection by Procamallanus (Spirocamallanus) inopinatus (Nematoda) in farmed Brycon cephalus (Characiformes) from Peru: a potential fish health problem. AQUACULTURE INTERNATIONAL, v. 28, n. 2, p. 13-pg., 2019-10-16. (16/13368-4, 18/20482-3, 15/23948-5)

MADRID, RAFAEL R. M.; MATHEWS, PATRICK D.; PATTA, ANA C. M. F.; GONZALES-FLORES, ANAI P.; RAMIREZ, CARLOS A. B.; RIGONI, VERA L. S.; TAVARES-DIAS, MARCOS; MERTINS, OMAR. Safety of oral administration of high doses of ivermectin by means of biocompatible polyelectrolytes formulation. HELIYON, v. 7, n. 1, JAN 2021. (18/20482-3, 16/13368-4, 15/23948-5, 17/16722-6)

LOPES, NATHALIE ALMEIDA; MERTINS, OMAR; BARRETO PINILLA, CRISTIAN MAURICIO; BRANDELLI, ADRIANO. Nisin induces lamellar to cubic liquid-crystalline transition in pectin and polygalacturonic acid liposomes. FOOD HYDROCOLLOIDS, v. 112, MAR 2021. (15/23948-5)

RAMIREZ, CARLOS A. B.; MATHEWS, PATRICK D.; MADRID, RAFAEL R. M.; GARCIA, IRENE T. S.; RIGONI, VERA L. S.; MERTINS, OMAR. Antibacterial polypeptide-bioparticle for oral administration: Powder formulation, palatability and in vivo toxicity approach. BIOMATERIALS ADVANCES, v. 153, p. 15-pg., 2023-10-01. (21/00971-2, 15/23948-5)

MATHEWS, PATRICK D. D.; PATTA, ANA C. M. F.; MADRID, RAFAEL R. M.; RAMIREZ, CARLOS A. B.; PIMENTA, BARBARA V. V.; MERTINS, OMAR. Efficient Treatment of Fish Intestinal Parasites Applying a Membrane-Penetrating Oral Drug Delivery Nanoparticle. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. 9, n. 6, p. 13-pg., 2021-11-15. (18/20482-3, 17/16722-6, 16/13368-4, 15/23948-5)

RIVADENEYRA, NORMA L. S.; MERTINS, OMAR; CUADROS, RUBEN C.; MALTA, JOSE C. O.; DE MATOS, LORENA V.; MATHEWS, PATRICK D.. Histopathology associated with infection by Procamallanus (Spirocamallanus) inopinatus (Nematoda) in farmed Brycon cephalus (Characiformes) from Peru: a potential fish health problem. AQUACULTURE INTERNATIONAL, OCT 2019. (16/13368-4, 18/20482-3, 15/23948-5)

MILANIN, TIAGO; MATHEWS, PATRICK D.; MERTINS, OMAR; TAVARES, LUIZ E. R.; SILVA, MARCIA R. M.; MAIA, ANTONIO A. M.. Molecular phylogeny of the gill parasite Henneguya (Myxosporea: Myxobolidae) infecting Astyanax lacustris (Teleostei: Characidae) from fish farm in Brazil. Microbial Pathogenesis, v. 123, p. 372-376, OCT 2018. (13/14656-5, 15/23948-5, 15/18807-3)

ESPINOZA, LUIS L.; MERTINS, OMAR; GAMA, GABRIELLA S.; FERNANDES PATTA, ANA C. M.; MATHEWS, PATRICK D.. A new Myxidium species (Myxozoa: Myxosporea) infecting the gallbladder of the turtle Podocnemis unifilis (Testudines: Podocnemididae) from Peruvian Amazon. Acta Tropica, v. 172, p. 75-79, AUG 2017. (15/23948-5, 16/25919-5)

DE MOURA, DIEGO SOARES; OLIVEIRA PAZINATO, JULIA CRISTINA; PEREIRA, MARCELO BARBALHO; MERTINS, OMAR; SILVA, EMERSON RODRIGO; SANTOS GARCIA, IRENE TERESINHA. Poly(vinyl alcohol) as a structuring agent for peroxotungstic acid. JOURNAL OF MOLECULAR LIQUIDS, v. 269, p. 92-100, NOV 1 2018. (15/23948-5)

GARCIA, BIANCA B. M.; MERTINS, OMAR; DA SILVA, EMERSON R.; MATHEWS, PATRICK D.; HAN, SANG W.. Arginine-modified chitosan complexed with liposome systems for plasmid DNA delivery. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 193, SEP 2020. (16/13368-4, 15/23948-5, 15/20206-8)

MATHEWS, PATRICK D.; MERTINS, OMAR; MILANIN, TIAGO; ESPINOZA, LUIS L.; FLORES-GONZALES, ANAI P.; AUDEBERT, FABIENNE; MORANDINI, ANDRE C.. Molecular Phylogeny and taxonomy of a new Myxobolus species from the endangered ornamental fish, Otocinclus cocama endemic to Peru: A host-parasite coextinction approach. Acta Tropica, v. 210, OCT 2020. (16/13368-4, 15/18807-3, 18/20482-3, 15/23948-5, 15/21007-9)

MADRID, RAFAEL R. M.; MERTINS, OMAR; TAVARES-DIAS, MARCOS; FLORES-GONZALES, ANAI P.; C.M.F. PATTA, ANA; RAMIREZ, CARLOS A. B.; RIGONI, VERA L. S.; MATHEWS, PATRICK D.. High compliance and effective treatment of fish endoparasitic infections with oral drug delivery nanobioparticles: Safety of intestinal tissue and blood parameters. JOURNAL OF FISH DISEASES, v. 44, n. 11, AUG 2021. (15/23948-5)

MATHEWS, PATRICK D.; MADRID, RAFAEL R. M.; MERTINS, OMAR; RIGONI, VERA L. S.; MORANDINI, ANDRE C.. A new Myxobolus (Cnidaria: Myxosporea) infecting the ornamental catfish Corydoras schwartzi from the Purus River in Brazil. EUROPEAN JOURNAL OF TAXONOMY, v. 620, p. 1-14, MAR 30 2020. (18/20482-3, 16/13368-4, 15/23948-5, 15/21007-9)

MATHEWS, PATRICK D.; MERTINS, OMAR. Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology. Carbohydrate Polymers, v. 157, p. 850-857, FEB 10 2017. (15/23948-5)

PAZINATO, JULIA C. O.; VILLETTI, MARCOS A.; MERTINS, OMAR; SILVA, EMERSON R.; GARCIA, IRENE T. S.. Insights on Structuration of Peroxotungstic Acid in Aqueous Media. Journal of the Brazilian Chemical Society, v. 30, n. 4, p. 752-763, APR 2019. (15/23948-5, 16/24409-3)

Filed patent(s) as a result of this research project