Characterization of bone tissue formed during the alveolar bone repair and osseointegration process in rats submited to anti-osteoporosis therapy. histological, histometrical, immunohistochemical, molecular and tridimensional aspects.

• Grant number: 15/14688-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Roberta Okamoto
• Grantee: Roberta Okamoto
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Alexandre Rodrigues Freire ; Ana Cláudia Rossi ; Felippe Bevilacqua Prado ; Leonardo Perez Faverani ; Mariza Akemi Matsumoto ; Paulo Roberto Botacin

Abstract

Osteoporosis is the most common bone tissue metabolic disorder in women in the postmenopausal period, caused by estrogen deficiency. Also affects men, but in a lower incidence and usually occurs by reducing the production of testosterone, which suffer the flavoring process into estrogen. In order to understand the alveolar bone and the bone around dental implants healing behavior , this research will investigate the action of anti-osteoporosis medications in rats. Therefore, young adult Wistar rats, males and females (n = 8 for each group and subgroup) will be divided according to the induced osteoporosis and anti-osteoporosis drug: The male rats will undergo bilateral orchiectomy, while females, to bilateral ovariectomy. As a positive control, a group of rats will be submitted only to the fictional surgery ovariectomy/orchiectomy (Sham). The animals will wait for 30 days to induce osteoporosis and thereby initiate drug therapy with strontium ranelate or human opg-fc by gavage or subcutaneously in rats, and teriparatide subcutaneously. After 30 days, the animals will be submitted to the extraction of the upper right incisor (wound healing) or the installation of implants in the tibia (peri-implant bone healing). Animals' Euthanasia will be at 14 and 42 days after extraction and only at 42 days after installation of implants, for analysis with decalcified tissue (histology and immunohistochemistry: OPG, RANKL, OC, TRAP, Wnt). A group of animals at 42 days before euthanasia will be anesthetized, made the reverse torque (N.cm) and subsequent collection of the peri-implant tissue for RNA extraction, cDNA preparation and PCR analysis of the expression array. In these same periods, other groups of animals will be submitted to fluorochromes administration at a dose of 20 mg/kg (calcein at 14 days and alizarin at 42 days) These will be euthanized 60 days after the extraction or the implant placement, for further laboratory procedures for calcified sections (microtomography, analysis of bone dynamics, BIC and NFBA). Quantitative data will be submitted to appropriate statistical test (parametric or non-parametric) with 5% significance level. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES-FERREIRA, P. H. S.; DE OLIVEIRA, D.; FRIGERIO, P. B.; DE SOUZA BATISTA, F. R.; GRANDFIELD, K.; OKAMOTO, R.. Teriparatide improves microarchitectural characteristics of peri-implant bone in orchiectomized rats. OSTEOPOROSIS INTERNATIONAL, v. 31, n. 9, MAY 2020. (15/14688-0, 15/04649-7)

SILVA GOMES-FERREIRA, PEDRO HENRIQUE; MICHELETTI, CHIARA; FRIGERIO, PAULA BUZO; DE SOUZA BATISTA, FABIO ROBERTO; MONTEIRO, NAARA GABRIELA; BIM-JUNIOR, ODAIR; LISBOA-FILHO, PAULO NORONHA; GRANDFIELD, KATHRYN; OKAMOTO, ROBERTA. PTH 1-34-functionalized bioactive glass improves peri-implant bone repair in orchiectomized rats: Microscale and ultrastructural evaluation. BIOMATERIALS ADVANCES, v. 134, p. 16-pg., 2022-03-01. (15/14688-0, 17/08187-3)

DE OLIVEIRA, DANILA; PUTTINI, IGOR DE OLIVEIRA; SILVA GOMES-FERREIRA, PEDRO HENRIQUE; PALIN, LETICIA PITOL; MATSUMOTO, MARIZA AKEMI; OKAMOTO, ROBERTA. Effect of intermittent teriparatide (PTH 1-34) on the alveolar healing process in orchiectomized rats. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 5, p. 2313-2322, MAY 2019. (15/14688-0, 15/20203-9)

GOMES-FERREIRA, PEDRO HENRIQUE SILVA; FRIGERIO, PAULA BUZO; DE MOURA, JULIANA; DUARTE, NATHALIA DANTAS; DE OLIVEIRA, DANILA; DEERING, JOSEPH; GRANDFIELD, KATHRYN; OKAMOTO, ROBERTA. Evaluation of Vitamin D isolated or Associated with Teriparatide in Peri-Implant Bone Repair in Tibia of Orchiectomized Rats. BIOLOGY-BASEL, v. 12, n. 2, p. 14-pg., 2023-02-01. (15/04649-7, 15/14688-0)